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Method for inducing tetraploid garlic by using oryzalin

A technique of amisulfralin and tetraploid, which is applied in the field of agriculture and can solve problems such as the induction of garlic tetraploid by amisulfralin

Active Publication Date: 2014-07-16
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, garlic has been successfully reported using colchicine to induce polyploidy (Zhang Suzhi et al., 2006), but there is no use of amisultraline to induce garlic tetraploidy

Method used

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  • Method for inducing tetraploid garlic by using oryzalin
  • Method for inducing tetraploid garlic by using oryzalin

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Embodiment 1

[0016] Mutagenesis treatment method: After the garlic bulbs are dried naturally to break dormancy, the peeled bulb buds are rinsed under tap water for 0.5 h, and then disinfected with 75% alcohol for 1 min under aseptic conditions, and the strips are peeled off under a dissecting microscope. The shoot tips of 2 leaf primordia with a diameter of about 0.2 mm were used as explants. Inoculate in B5+6-BA1.0mg / L+NAA0.5mg / L liquid medium containing 2-12mg / L amisulfamelan, culture in dark until the set treatment time is 48-144h; then take out the shoot tip Rinse in sterile water for 3-5 times, then transfer the shoot tips to solid medium, the culture conditions are temperature 23.0±2.0℃, light 25~31μmol·m-2·s -1 , 16h / d.

[0017] Tetraploid identification method: the ploidy identification of the obtained plants is carried out by two methods of flow cytometry detection and chromosome counting. A tetraploid induction rate of 25.6% was obtained. Compared with colchicine as a control,...

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Abstract

The invention discloses a method for inducing tetraploid garlic by using oryzalin. According to the method, the oryzalin is selected as a mutagenic agent for inducing the tetraploid garlic, an optimal mutagenizing method is adopted, and the best effect of inducing tetraploid garlic is obtained through research, and thus germplasm resource reserve is provided for germplasm innovation and breeding of new variety of the garlic.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and in particular relates to a method for inducing garlic tetraploid by using amisulfameline. Background technique [0002] Colchicine is a commonly used chemical mutagen that induces chromosome doubling. Compared with colchicine, amisulfameline has less toxicity and better chromosome doubling effect. At present, amisulfamelan has successfully induced polyploidy in a variety of crops (Chalak1996; 2000; Carvalho et al. 2005; M.M.et al., 2011), and also used in potato (Leire Barandalla et al., 2006), rose (J.F.Allum et al., 2007), Hubei Bauhinia (Joshua D.Nadlera et al., 2012), lily ( Jian Jia et al., 2013), ornamental ginger (HamidouF., 2009), watermelon (Yan Zhihong et al., 2008; Zhao Yijie et al., 2011), ponkan (He Jian et al., 2011) orchid (TildenP. et al., 2011) and other crops. The invention patent (Application No. CN201310258679) of "A Method for Inducing Tetraploid of Thin-skinn...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 孔素萍徐坤刘波高莉敏刘冰江刘贤娴陈伟
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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