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Identification method of salt-tolerant resource in seedling period of cowpea

An identification method, the technology of cowpea, applied in the field of ecological environment

Inactive Publication Date: 2016-02-17
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

There is no report on the identification and evaluation of germplasm resources in cowpea seedling stage

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  • Identification method of salt-tolerant resource in seedling period of cowpea
  • Identification method of salt-tolerant resource in seedling period of cowpea
  • Identification method of salt-tolerant resource in seedling period of cowpea

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Embodiment Construction

[0032] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0033] The present invention has established a kind of taking vermiculite as matrix, with 400mmol·L –1 NaCl treatment, a simple method for identification of salt tolerance at the seedling stage of cowpea within 15 days, provides a new method for the large-scale identification of seedling salt tolerance of cowpea germplasm resources, the selection of salt-tolerant varieties and the study of salt-tolerant mechanisms.

[0034] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0035] Such as figure 1 As shown, the cowpea seedling stage salt-tole...

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Abstract

The invention discloses an identification method of a salt-tolerant resource in the seedling period of cowpea, which is easy to operate and reliable in identification result. The identification method comprises the steps of carrying out germination experiment by adopting one cowpea variety to screen the optimal ultrasonic treatment method and determine the germination conditions; carrying out salt treatment experiment on three varieties by adopting salt solutions of multiple gradients to determine the optimal treatment condition; carrying out salt-tolerant experiment on 18 cowpea varieties of different genes, and carrying out principal component analysis to determine the optimal regression model. The identification method is used for identifying the salt-tolerant capability of the cowpea with 15d, and is short in identification time; the optimal salt-tolerant indexes of the cowpea are plant height, root length, plant dry weight and root dry weight, the identification method with simple operation and definite index is established, the shape indexes are visualized and convenient to count, and the standard identification method is provided for large-scale rapid identification of salt tolerance in the seedling period of the germplasm resource of the cowpea as well as the salt-tolerant variety selection and salt-tolerant mechanism.

Description

technical field [0001] The invention belongs to the technical field of ecological environment, and in particular relates to a method for identifying salt-tolerant resources at the seedling stage of cowpea. Background technique [0002] According to incomplete statistics from the United Nations Educational, Scientific and Cultural Organization (UNESCO) and the United Nations Food and Agriculture Organization (FAO), the saline soil area in the world is about 1 billion hectares 2 (Wang Zunqin, 1993). The area of ​​saline soil in my country is about 100 million hm 2 , these saline soils are part of my country's important land resources, especially in the northern and southern coastal areas. A large number of research results show that biological measures to screen and utilize new species of salt-tolerant plants is one of the most economical and effective methods for improving saline-alkali land (Shi Jinxia et al., 2004; Stapies, 1984). Since the indoor identification of salt ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01C1/00
CPCA01G7/00A01C1/00
Inventor 张红梅刘晓庆陈华涛陈新崔晓艳袁星星
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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