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Carnation new specie cultivation method

A cultivation method and technology of carnation, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as new varieties that are rarely cultivated independently, shorten the identification time, improve the mutagenesis rate, and improve the survival rate. Effect

Inactive Publication Date: 2019-06-07
中国科学院昆明植物研究所海盐工程技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The Chinese carnation native to our country also has high ornamental value, but most of the popular varieties in the domestic market are imported foreign varieties, and there are very few new varieties cultivated independently

Method used

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  • Carnation new specie cultivation method

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Effect test

Embodiment 1

[0029] A method for cultivating a new variety of carnation, the specific method is as follows: Carnation is subjected to in vitro tissue culture, a chemical mutagen is used to induce polyploid production, and a new polyploid carnation variety is cultivated. The cultivation method combines tissue culture technology and chemical mutagenesis doubling method, uses mutagen to induce intracellular chromosome doubling at the level of isolated tissues, improves the induction rate, and the experimental conditions are easy to control, the results are reproducible, and at the same time, chimeras can be reduced Incidence rate, easy to purify, and the new polyploid Carnation variety cultivated can form a stable variety after asexual propagation.

[0030] The chemical mutagen contains a mixed solution of colchicine, asulphine and penetrant. The preparation steps are as follows: respectively take 0.01g of colchicine and 0.03g of asulphin and dissolve it in 1g of penetrant, then add 100ml of d...

Embodiment 2

[0040] A kind of cultivation method of carnation new variety, concrete steps are as follows:

[0041] 1) Preparation of chemical mutagen: Dissolve 0.02g of colchicine and 0.04g of asulphin in 1g of penetrant, and then add 120ml of distilled water to dissolve to obtain the mother liquor, and then dilute the mother liquor with distilled water to a concentration of 0.6mg / l and 0.07mg / l, and then sterilized at 125°C for 25 minutes, and after cooling, different concentrations of chemical mutagens can be obtained. The above-mentioned penetrating agent contains dimethyl sulfoxide and crown ether, and the crown ether is contained in the penetrating agent The weight ratio of the crown ether is 0.035%, and the weight ratio of 18-crown ether-6 and 15-crown ether-5 in the crown ether is 1.5:1;

[0042] 2) Obtain callus tissue: take the side buds of carnation that grow robustly, remove the leaves, sterilize with 85% alcohol for 40 seconds, then sterilize with 0.2% mercury chloride solution...

Embodiment 3

[0048] A kind of cultivation method of carnation new variety, concrete steps are as follows:

[0049] 1) Preparation of chemical mutagen: Dissolve 0.03g of colchicine and 0.04g of asulphin in 2g of penetrant, and then add 110ml of distilled water to dissolve to obtain the mother liquor, and then dilute the mother liquor with distilled water to a concentration of 0.7mg / l and 0.09mg / l, and then sterilized at 120°C for 20 minutes, and after cooling, different concentrations of chemical mutagens can be obtained. The above-mentioned penetrating agent contains dimethyl sulfoxide and crown ether, and the crown ether is contained in the penetrating agent The weight ratio of the crown ether is 0.03%, and the weight ratio of 18-crown ether-6 and 15-crown ether-5 in the crown ether is 2:1;

[0050]2) Obtain callus tissue: take the side buds of healthy Carnation, remove the leaves, sterilize with 80% alcohol for 35 seconds, then sterilize with 0.1% mercury chloride solution for 7 minutes,...

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Abstract

The invention discloses a carnation new specie cultivation method, and relates to the technical field of plant culture. The method includes the steps: culturing a carnation explant, placing the explant into a culture medium containing mutagenic agents with high concentration to induce the explant after a callus tissue is formed; transferring the explant into the culture medium containing mutagenicagents with lower concentration to perform multiplication; transferring the explant into the culture medium without mutagenic agents with lower concentration to perform rooting culture; transplantingrotting seedlings to obtain transplanting seedlings, culturing the transplanting seedlings into plates, identifying the plants by morphology, cytology and chromosome number, and determining an selecting carnation new specie. The mutagenic agents comprise mixed solution of colchicine, oryzalin dimethyl sulfoxide and crown ether. The invention provides a carnation new specie cultivation method. Themethod is high in operability and repeatability, directed variation can be achieved, chimeras are decreased, polyploid variation rate is increased, vitrification is reduced, subpolyploids are stable,identifying time is shortened, and the survival rate of the mutant seedlings is high.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for cultivating a new carnation variety. technical background [0002] Carnation, also known as carnation and carnation, is a perennial herbaceous plant of the family Caryophyllaceae. Carnation originated in southern Europe, the Mediterranean, France and Greece. At present, carnation has become one of the most important cut flower varieties commercially cultivated in the contemporary world. In the European market, carnation is the second largest flower after rose, accounting for 20% of fresh cut flower exports. Carnation native to China also has high ornamental value and is one of the main parents of modern cut carnation. The cultivation of carnation in my country began in the early 20th century, and the main producing areas are Shanghai, Yunnan, Guangzhou and other places. Yunnan is one of the three most suitable areas for carnation cultivation in the world. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 鲁元学冷容李玲石勇宋红玉
Owner 中国科学院昆明植物研究所海盐工程技术中心
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