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Method for cultivating pelargonium graveolens polyploids

A technology of geranium geranium and cultivation method, applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc., can solve problems such as complicated operation process, high cost, and many variants, and achieve improved germination The effect of increasing the rate, improving optional traits, and stabilizing polyploid traits

Inactive Publication Date: 2019-06-14
金华市飞凌生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to foreign literature reports, the current method for obtaining polyploid plants of Geranium geranium is: in the process of tissue culture, through the culture of petioles, roots, stems and other tissues of Geranium geranium, first induce callus and then differentiate into adventitious buds , polyploid plants can be screened from adventitious buds, but this method has many offspring variants, complicated operation process, requires a variety of culture media, and the cost is high
There are few domestic studies on the induction of polyploidy in Pelargonium geranium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The present embodiment is a polyploid cultivation inducing aid, and the following items in the inducing aid can be present in the inducing aid in the following weight percentages based on the total amount of the inducing aid:

[0038] (a) 80wt% amisulfurin as the main component;

[0039] (b) a mixture of Miglitol and chelleric acid in a specific weight ratio of 20wt%;

[0040] Wherein, the specific weight ratio of the miglitol and chelleric acid is 25:1.

[0041] Amisulidine can inhibit cell mitosis, destroy the spindle body, and cause chromosomes to stagnate in the metaphase. In such mitosis, although the chromosomes are longitudinally split, the cells do not divide and cannot form two daughter cells, so the chromosomes are doubled, and aminosulfurine can be used. However, the efficiency of obtaining polyploids by using amisulfuryl solution alone is relatively low, and its successful induction rate can only reach 40-50%, and a large amount of screening work needs to b...

Embodiment 2

[0055] A method for cultivating geranium polyploids, comprising:

[0056] Preparation: In the dark, at a temperature of 2°C, dissolve the induction aid in a 3wt% glucose solution at a mass content of 80 mg / L to obtain the induction solution; dissolving the induction aid in the glucose solution will help increase the induction aid The degree of dispersion is conducive to the smooth entry of the active ingredients in the induction aids into the seeds of Geranium geranium in the later induction process to complete the induction cultivation;

[0057] Induction: Place the sterilized Pelargonium geranium seeds in the induction solution, keep the seeds under the liquid level all the time, induce culture for 14 days in the dark, at a temperature of 25°C, and at 120r / min, wash and remove the solution on the surface of the seeds; avoid light, Under the shaker environment, the seeds of Geranium geranium are induced with the induction solution containing the induction auxiliary agent, and...

Embodiment 3

[0071] [i] A polyploid cultivation induction aid, said induction aid comprising:

[0072] (a) Amisulamide as the main ingredient;

[0073] (b) a mixture of Miglitol and chelleric acid in a specific weight ratio;

[0074] Wherein, the specific weight ratio of the miglitol and chelleric acid is 25:1.

[0075] The following items in the induction aid can be present in the induction aid in the following weight percentages based on the total amount of the induction aid:

[0076] (a) 99wt% of amisulfurin as the main component;

[0077] (b) a mixture of Miglitol and chelleric acid in a specific weight ratio of 1wt%;

[0078] Wherein, the specific weight ratio of the miglitol and chelleric acid is 25:1.

[0079] Amisulidine can inhibit cell mitosis, destroy the spindle body, and cause chromosomes to stagnate in the metaphase. In such mitosis, although the chromosomes are longitudinally split, the cells do not divide and cannot form two daughter cells, so the chromosomes are double...

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PUM

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Abstract

The invention provides a method for cultivating pelargonium graveolens polyploids, and belongs to the technical field of flower cultivation. The method comprises the steps of 1) preparation, wherein an inducing solution is obtained by dissolving an inducing aid in a glucose solution; 2) inducing, wherein pelargonium graveolens seeds are placed in the inducing solution for inducing culture; 3) germination, wherein the induced pelargonium graveolens seeds are transferred to a germination culture medium for culture to obtain polyploid seedlings; 4) expanding propagation, wherein the polyploid seedlings are selected and transferred into a proliferation culture medium for expanding propagation and identification; 5) rooting, wherein plants which are identified to be polyploids are transferred into a rooting culture medium for rooting culture to obtain seedlings, and the seedlings are transplanted. The method has the advantages that compared with a mode of only using oryzalin, the inducing success rate is significantly increased, the polyploid traits are stable, the increase of the content of chimeras in the polyploids is also promoted, pelargonium graveolens with more excellent and specific color, shape, taste and the like is obtained accordingly, the roots of the seedlings are strong after cultivation, and the survival rate after transplanting is high.

Description

technical field [0001] The invention belongs to the technical field of flower cultivation, and in particular relates to a method for cultivating geranium geranium polyploids. Background technique [0002] Geranium geranium (scientific name: Pelargonium graveolens L'Herit.), is a perennial herb or shrub, up to 1m high. Stem erect, base lignified, upper part fleshy, densely covered with shiny pilose, fragrant. Native to southern Africa, especially South Africa; likes warm, humid and sunny environments. Poor cold resistance, afraid of water humidity and high temperature. Geranium geranium is popular because of the volatile aroma of the plant, which can be used to make essential oils, and its main ingredients are geraniol, citronellol, linalool and so on. Geranium Geranium has a variety of scents, including rose, citrus, mint, coconut and bean curd, as well as a variety of fruit flavors. Geranium geranium likes temperature and drought, is not cold-tolerant, is afraid of wate...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H4/00A01N43/90A01N43/16A01N41/06A01P21/00
Inventor 金韶京
Owner 金华市飞凌生物科技有限公司
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