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Strawberry chromosome doubling method and rapid identification method for used ploidy

A chromosome ploidy and chromosome doubling technology, applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems such as no reports on the application of amisulfalil, and achieve the effect of high accuracy

Active Publication Date: 2019-01-11
HANGZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the application of amisulfamelan on strawberries in China

Method used

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  • Strawberry chromosome doubling method and rapid identification method for used ploidy
  • Strawberry chromosome doubling method and rapid identification method for used ploidy
  • Strawberry chromosome doubling method and rapid identification method for used ploidy

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Example 1: A method for rapid identification of strawberry chromosome doubling and ploidy, in which the following steps are performed in sequence:

[0056] 1) Take the stolons of wild forest strawberry (diploid) that are robust and free of diseases and insect pests, and take the length of 1~2cm at the tip of the stem as the tip of the stem, and rinse with running water (put it in a gauze bag and rinse with tap water);

[0057] 2) After sterilizing the stem apex after washing with the above running water (conventional disinfection), cut 3.0~5.0 mm as the stem apex and inoculate it on the medium for inducing sterile seedlings for cultivation; the culture condition is: 16 hours of light ,Light intensity 30~40μmol m -2 ·S -1 , The temperature is 25 ± 1 ℃; 8 hours of dark culture, the temperature is 21 ± 1 ℃; the above light and dark culture alternately; until the 1.0 ~ 2.0 cm high sterile seedlings are obtained;

[0058] Induction of sterile seedling medium: MS basic medium + 0.5m...

Embodiment 2

[0087] Example 2. Double the concentration of amisulin in the mutagenesis medium of Example 1 from 200.0 μmol.﹒ L -1 Change to 150μmol. L -1 , The rest is equivalent to Example 1.

[0088] The yield of induced double test-tube vaccine is 1.5-2%.

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Abstract

The invention discloses a rapid identification method for the ploidy of a strawberry chromosome. The rapid identification method comprises the steps: taking a mutation-induced strawberry obtained by inducing a wild forest strawberry as a test material and a diploid wild forest strawberry as a contrast; preparing a test material stained cell nucleus suspension from the test material, and preparinga contrast stained cell nucleus suspension from a contrasted leaf; and detecting the two suspensions on a flow cytometer, analyzing the ploidy of the test material by utilizing a histogram for the relative content of DNA, and screening a ploidy-variant individual plant. The invention simultaneously provides a strawberry chromosome doubling method realized by using the rapid identification method for the ploidy of the strawberry chromosome, and the chromosome of the diploid wild forest strawberry is doubled by using oryzalin in the method. By using the method disclosed by the invention, a strawberry doubling progeny can be effectively obtained.

Description

Technical field [0001] The invention relates to a method for doubling diploid wild strawberry by using asulfuryl and quickly identifying chromosome ploidy by using a cell flow cytometer. Background technique [0002] Strawberry is a perennial evergreen plant, belonging to the Rosaceae (Rosaceae) Fragaria (Fragaria), the chromosome base is x=7, and its chromosome ploidy includes diploid (2x), tetraploid (4x), and pentaploid ( 5x), hexaploid (6x), octoploid (8x) and a small amount of decaploid (10x) and dodecaploid (12x). At present, the strawberry varieties commercially grown all over the world belong to the octoploid (2n=8x=56) large-fruit pineapple strawberry (Fragaria×ananassa Duch.). There are about 20 common species of strawberry in the world, except for the large-fruit cultivated pineapple. Except for strawberry, the other species are wild. Wild strawberry has many excellent characteristics, especially in cold resistance, disease resistance, solid content, aroma, high cont...

Claims

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Application Information

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IPC IPC(8): G01N15/14A01H1/08A01H4/00C12Q1/04
CPCA01H1/08A01H4/001G01N15/1434
Inventor 王淑珍余红柴伟国周历萍来文国童建新
Owner HANGZHOU ACAD OF AGRI SCI
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