Efficient establishment method for Chinese cymbidium sexual polyploid
A polyploid and national orchid technology, applied in horticultural methods, botany equipment and methods, plant genetic improvement, etc., can solve the problems of low polyploid survival rate, difficult direct use, slow growth, etc., and achieve economic benefits and The effect of obvious social benefits, strong growth potential and fast growth speed
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[0035] Example 1: Creation of Molan Sexual Polyploid Resources
[0036] Since 2012, we have used the method described in the present invention to create Molan sexual polyploid resources. The specific method is as follows (the process is as follows: figure 1 shown):
[0037] S1. Parent selection and hybrid combination configuration: select 06-bs-45-32 (2n male gamete incidence rate 2.67) and 06-bs-45-17 (2n male gamete incidence rate 3.92) with high incidence of 2n male gametes as Parent material, on February 20, 2012, 06-bs-45-32 and 06-bs-45-17 were used as female parents, and 06-bs-45-17 was used as male parent to prepare a hybrid combination, harvested on October 18 of the same year fruit.
[0038] Among them, the methods for obtaining 06-bs-45-32 (incidence of 2n male gametes: 2.67) and 06-bs-45-17 (incidence of 2n male gametes: 3.92) with high incidence of 2n male gametes refer to the content disclosed in patent 201310668763.9.
[0039] S2. Seed culture and identifica...
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