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Method for inducing chill-proof Aglaonema spp polyploidy

A polyploidy and bud induction technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as frostbite, affecting production and sales, and achieve lower heating costs, beautiful plant shapes, and improved economic benefits. Effect

Inactive Publication Date: 2012-05-09
东莞市生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, frostbite generally occurs below 15°C, which affects its production and sales in winter.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Step 1. Induction of clumping buds: Take the stems or apical buds of S. crassicarpa plant without pests and diseases as explants, scrub it with saturated detergent solution, rinse off the detergent with running water, and put it on the ultra-clean workbench. The explants are immersed in a 0.1% mercury solution containing a few drops of Tween-20 for 15-30 minutes, rinsed with sterile water for 4-5 times, and the surface water is absorbed with sterile filter paper, and then inserted into the clump bud induction medium , Placed at a temperature of 23℃, light for 10h·d-1, and light intensity of 60μmol m -2 s -1 The cultivation room induces clumping buds. The clump bud induction medium is: MS+2-IP1mg / L+NAA0.2mg / L+white sugar 25g / L+carrageenan 6g / L, pH5.5.

[0018] Step two, colchicine treatment: remove the clump buds together with the calluses below, place them in a 0.2% colchicine solution and shake culture for 48 hours in the dark, rinse with sterile water for 4-5 times, and ...

Embodiment 2

[0025] Step 1. Induction of clumping buds: Take the stems or apical buds of S. crassicarpa plant without pests and diseases as explants, scrub it with saturated detergent solution, rinse off the detergent with running water, and put it on the ultra-clean workbench. The explants are immersed in a 0.1% mercury solution containing a few drops of Tween-20 for 15-30 minutes, rinsed with sterile water for 4-5 times, and the surface water is absorbed with sterile filter paper, and then inserted into the clump bud induction medium , Placed at a temperature of 25℃, light for 11h·d-1, light intensity of 80μmol m -2 s -1 The cultivation room induces clumping buds. The clump bud induction medium is: MS+2-IP2mg / L+NAA0.3mg / L+white sugar 30g / L+carrageenan 6.5g / L, pH5.8.

[0026] Step two, colchicine treatment: remove the clump buds together with the callus below, place them in a solution containing 0.3% colchicine and shake culture for 60 hours in the dark, rinse with sterile water for 4-5 time...

Embodiment 3

[0033] Step 1. Induction of clumping buds: Take the stems or apical buds of S. crassicarpa plant without pests and diseases as explants, scrub it with saturated detergent solution, rinse off the detergent with running water, and put it on the ultra-clean workbench. The explants are immersed in a 0.1% mercury solution containing a few drops of Tween-20 for 15-30 minutes, rinsed with sterile water for 4-5 times, and the surface water is absorbed with sterile filter paper, and then inserted into the clump bud induction medium , Placed at a temperature of 27℃, light for 12h·d-1, and light intensity of 100μmol m -2 s -1 The cultivation room induces clumping buds. The clumping bud induction medium is: MS+2-IP3mg / L+NAA0.5mg / L+white sugar 35g / L+carrageenan 7g / L, pH6.5.

[0034] Step two, colchicine treatment: remove the clump buds together with the callus below, place them in a solution containing 0.3% colchicine and shake culture for 72 hours in the dark, rinse with sterile water for 4-...

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Abstract

The invention relates to a method for inducing a chill-proof Aglaonema spp polyploidy, relating to an Aglaonema spp breeding technology, which comprises the following steps of: inducing cluster buds, colchicine treating, carrying out low-temperature treatment, propagating variation plants, identifying the polyploidy, rooting and transplanting, wherein after being subjected to five generations of propagation, the variation plants are subjected to polyploidy detection, thus, the proportion of a chimera can be effectively reduced; the stems of the polyploidy plants are thickened and stunted in comparison with common stems, thus, the shapes are more attractive; the polyploidy plants have the chill-proof property and can resist 5 DEG C without cold injury; the temperature increasing cost of a greenhouse in winter is reduced; and the economical benefit is greatly increased.

Description

Technical field [0001] The present invention relates to the breeding technology of rough grass, in particular to a method for inducing cold-resistant rough grass polyploid. Background technique [0002] Gracilaria is the general name of Aglaonema (Aglaonema) plant, also known as Guangdong Dieffenbachia, Bright Silk Grass, native to Guangdong, Guangxi, Yunnan and Southeast Asia in India, Thailand, Vietnam, Philippines, Malaysia, Indonesia and New Guinea and other places are perennial evergreen plants. Its leaves, petioles and veins have a variety of colors and have a strong appreciation value. They are deeply loved by people and are listed as ideal indoor foliage plants in the world. However, frostbite generally occurs below 15°C, which affects its winter production and sales. For this reason, by inducing polyploidy, cultivating a new variety of rough grass with cold resistance is of great significance to meet the needs of the market. Summary of the invention [0003] The presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 范俊强郑贵朝
Owner 东莞市生物技术研究所
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