Method for inducing chill-proof Aglaonema spp polyploidy

A technology of polyploidy and bud induction, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as frostbite, affecting production, sales, etc., and achieve reduced heating costs, beautiful plant shape, and improved economic benefits Effect

Inactive Publication Date: 2012-11-07
东莞市生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, frostbite generally occurs below 15°C, which affects its production and sales in winter.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Step 1. Induction of clustered buds: take the stems or terminal buds of non-pest and disease-free and robust Grass pratense plants as explants, first scrub them with saturated washing powder solution, rinse off the washing powder with running water, and place them on the ultra-clean workbench. Soak the explants in 0.1% mercuric chloride solution containing a few drops of Tween-20 for 15-30min, rinse with sterile water 4-5 times, blot the surface water with sterilized filter paper, and then insert it into the cluster bud induction medium , placed at a temperature of 23°C, and illuminated for 10h·d -1 , with an illumination intensity of 60 μmol m -2 the s -1 Cluster buds were induced in the culture chamber of the . Cluster bud induction medium is: MS+2-IP1mg / L+NAA0.2mg / L+sugar 25g / L+carrageenan 6g / L, pH5.5.

[0017] Step 2, colchicine treatment: remove the top of the clustered buds together with the callus below, place them in a solution containing 0.2% colchicine for ...

Embodiment 2

[0024] Step 1. Induction of clustered buds: take the stems or terminal buds of non-pest and disease-free and robust Grass pratense plants as explants, first scrub them with saturated washing powder solution, rinse off the washing powder with running water, and place them on the ultra-clean workbench. Soak the explants in 0.1% mercuric chloride solution containing a few drops of Tween-20 for 15-30min, rinse with sterile water 4-5 times, blot the surface water with sterilized filter paper, and then insert it into the cluster bud induction medium , placed at a temperature of 25°C, and illuminated for 11h·d -1 , with an illumination intensity of 80 μmol m -2 the s -1 Cluster buds were induced in the culture chamber of the . Cluster bud induction medium is: MS+2-IP2mg / L+NAA0.3mg / L+sugar30g / L+carrageenan6.5g / L, pH5.8.

[0025] Step 2, colchicine treatment: remove the top of the clustered buds together with the callus below, place them in a solution containing 0.3% colchicine for ...

Embodiment 3

[0032] Step 1. Induction of clustered buds: take the stems or terminal buds of non-pest and disease-free and robust Grass pratense plants as explants, first scrub them with saturated washing powder solution, rinse off the washing powder with running water, and place them on the ultra-clean workbench. Soak the explants in 0.1% mercuric chloride solution containing a few drops of Tween-20 for 15-30min, rinse with sterile water 4-5 times, blot the surface water with sterilized filter paper, and then insert it into the cluster bud induction medium , placed at a temperature of 27°C, and illuminated for 12h·d -1 , with an illumination intensity of 100 μmol m -2 the s -1 Cluster buds were induced in the cultivation chamber of the . Cluster bud induction medium is: MS+2-IP3mg / L+NAA0.5mg / L+sugar35g / L+carrageenan7g / L, pH6.5.

[0033]Step 2, colchicine treatment: remove the tops of the clustered buds together with the callus below, place them in a solution containing 0.3% colchicine f...

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PUM

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Abstract

The invention relates to a method for inducing a chill-proof Aglaonema spp polyploidy, relating to an Aglaonema spp breeding technology, which comprises the following steps of: inducing cluster buds, colchicine treating, carrying out low-temperature treatment, propagating variation plants, identifying the polyploidy, rooting and transplanting, wherein after being subjected to five generations of propagation, the variation plants are subjected to polyploidy detection, thus, the proportion of a chimera can be effectively reduced; the stems of the polyploidy plants are thickened and stunted in comparison with common stems, thus, the shapes are more attractive; the polyploidy plants have the chill-proof property and can resist 5 DEG C without cold injury; the temperature increasing cost of a greenhouse in winter is reduced; and the economical benefit is greatly increased.

Description

technical field [0001] The present invention relates to the breeding technique of the rough grass, in particular to a method for inducing the polyploid of the cold-resistant rough grass. technical background [0002] Aglaonema is a general term for plants of the genus Aglaonema in the family Araceae, also known as Guangdong Evergreen and Bright Silk Grass. It is native to Guangdong, Guangxi, Yunnan and India, Thailand, Vietnam, the Philippines, Malaysia, Indonesia and Southeast Asia. In New Guinea and other places, it is a perennial evergreen herb. Its leaves, petioles and leaf veins have a variety of color combinations, have a strong appreciation value, are deeply loved by people, and are listed as ideal indoor foliage plants internationally. However, frostbite generally occurs at temperatures below 15°C, affecting production and sales in winter. For this reason, it is of great significance to meet the needs of the market to breed new varieties of Sclerophyllum with cold ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 范俊强郑贵朝
Owner 东莞市生物技术研究所
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