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Method for culturing lily triploid

A cultivation method, triploid technology, applied in the direction of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of not being able to obtain triploid, etc., and achieve the effect of strong vegetative growth advantage

Inactive Publication Date: 2005-02-16
云南雷森园林有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Using colchicine to treat scales and test-tube plantlets, only autotetraploids can be obtained, but triploids cannot be obtained
The conventional method to obtain triploids is to cross tetraploids with diploids, but this method takes at least 5 years to obtain triploid lilies, and the cultivation technology for obtaining triploid lilies in a shorter time has not been reported yet.

Method used

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Embodiment Construction

[0013] Choose robust and disease-free bulbs with a diameter of 14-16 cm, and perform vernalization at a low temperature of 2-5 °C for 90 days according to conventional lily cultivation techniques to break dormancy and complete vernalization, and then cultivate them in a sterilized substrate; The environmental conditions are temperature 20-25°C, light 50-60,000 Lnx, and air humidity 50-60%. Under the condition of temperature of 20-25°C, the flower buds are treated with drip method for 2-3 days, at 8-10 am and 2-4 pm every day, to induce 2n female gametes, and then wash the treated parts with water; For the above plants, strengthen field management to ensure a good growth environment. According to conventional cultivation techniques, flowering will take about 50-60 days after treatment. The treated plants are not 100% mutated, only 9.52-25.8%. big different. Only mutated plants can produce 2n gametes. The identification of 2n gametes is mainly determined by morphological observ...

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Abstract

The invention relates to a plant variety cultivating method, especially a method of cultivating lily polyploidy. 1. according to routine lily cultivating technique, selecting seed balls and jarovizing at low temperature to break dormancy to complete the jarovizing; 2. cultivating in a greenhouse or making field cultivation and management according to routine cultivating lily technique; 3. cultivating for 30-40days, then dripping 0.02-0.2% colchicines at 20 deg.C-25 deg.C; 4. according to the routine cultivating technique, processing the plants so that they will come into flower in 50-60 days, selecting the plants with extremely large stigmas and huge direct microscopic pollen particles to eliminate stamens so as to obtain 2n-oogamete containing stamens; 5. gathering normal pollens to hybridize with the obtained stamens, then according to routine cultivating technique, cultivating so that the fruits will swell fully in 45-60 days, thus able to gather them; 6. adopting routine triploid embryo in vitro culture method to be able to obtain the lily triploids.

Description

technical field [0001] The invention relates to a method for cultivating plant varieties, in particular to a method for cultivating lily polyploids. technical background [0002] Lily is a plant of the genus Lilium in the family Liliaceae. It has large flowers, rich colors, and beautiful flowers. Especially Oriental hybrids have strong fragrance and are deeply loved by people. Lily cut flowers are currently one of the fastest growing flowers in the world, with broad market prospects. [0003] Polyploidy is one of the ways of plant evolution. Polyploidy has the characteristics of giganticity, low fertility, stress resistance and overcoming the sterility of distant hybridization, especially triploidy has specific qualities and powerful It is favored by horticultural breeders for its vegetative growth advantages. Studies on polyploidy in lilies have been reported, and it was found that tetraploid plants are taller and have more flowering substances. Lily breeding technology ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 郑思乡黄静张力
Owner 云南雷森园林有限公司
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