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Method for obtaining polyploidy paulownia tomentosa by performing tissue culture propagation by taking young flowers as explants

A technology of polyploid and explant, which is applied in the field of new tree variety selection and breeding in modern forestry, and can solve the problems of fewer plants, damage to the original plant, and low reproduction efficiency

Active Publication Date: 2014-04-09
HUBEI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few original plants of polyploid Paulownia tomentosa, and the plants have grown to the age of flowering. Propagation with root segments not only seriously damages the original plants, but also has low reproduction efficiency.

Method used

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  • Method for obtaining polyploidy paulownia tomentosa by performing tissue culture propagation by taking young flowers as explants
  • Method for obtaining polyploidy paulownia tomentosa by performing tissue culture propagation by taking young flowers as explants

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Embodiment Construction

[0023] The present invention will be further described below with the tissue culture propagation of the polyploid Paulownia tomentosa No. 1 of the embodiment.

[0024] a. Selection of young flowers

[0025] The newly selected polyploid paulownia tomentosa strain No. 1 was used as the material, and the flowering branches were picked in early December 2012, and the flowering branches were full of yellowish-brown spindle-shaped young flowers.

[0026] b. Pretreatment and disinfection of young flowers

[0027] Insert the recovered flower branches into vases filled with hot water at 35°C-40°C, cover them with plastic bags, and place them in a tissue culture room at 26°C-27°C for 4 days. Pick the young flowers, cut them with a scalpel on an ultra-clean workbench to remove the yellow-brown calyx of the young flowers; then put the young flowers in 0.1% mercury chloride for 18 min, wash them with sterile water for 3 times, and cut them vertically and horizontally Open and cut into sm...

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Abstract

The invention provides a method for obtaining polyploidy paulownia tomentosa by performing tissue culture propagation by taking young flowers as explants. The method comprises the steps of a, selecting the young flowers; b, pretreating and disinfecting the young flowers; c, performing callus induction on the young flowers; d, performing callus differentiation culture; e, differentiating seedlings to obtain roots; f, transplanting tissue culture seedlings; and g, observing and authenticating tissue culture plants. By the adoption of the young flowers serving as the explants, the method has the outstanding advantages of high regeneration capability and polyploidy tissue culture propagation in the period from winter to early spring; the problems that the root propagation rate of the polyploidy paulownia tomentosa is low and the leaf tissue culture propagation seedling differentiation frequency is low are solved; the polyploidy paulownia tomentosa can be propagated in a large scale; the authenticity and the consistency of polyploidy of the tissue culture offspring are determined by a morphology and cytology technology.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of polyploid paulownia tomentosa by using young flowers as explants, and belongs to the technical field of new forest variety selection and breeding in modern forestry. Background technique [0002] Paulownia is an excellent fast-growing tree species, with early flowers and high ornamental value. However, for a long time, Paulownia has been propagated by root segments. This long-term root propagation is easy to be infected with "arbush disease", which affects the quality of forest materials and loses its ornamental value. In particular, the applicant used the patented technology 200810048497.9 (in vitro culture and colchicine treatment combined with the method of breeding polyploid Paulownia tomentosa) to breed a new strain of polyploid Paulownia tomentosa, which has thicker leaves, rounder leaves, and larger flowers. Excellent characteristics (Hubei Provincial Scientific and Technol...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 蔡得田宋兆建王维张献华何玉池刘育华
Owner HUBEI UNIV
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