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Cultivation method for polyploidy siraitia grosvenorii plant

A cultivation method, the technology of Luo Han Guo, applied in the field of plant genetics and breeding, can solve the problems of low mutagenesis efficiency and plant survival rate, and achieve good cell division inhibition effect, fast germination, and good cultivation effect

Inactive Publication Date: 2015-04-29
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current research on Luo Han Guo, the method of chromosome doubling by dropping colchicine on the bud tips of field plants is used, and the mutagenesis efficiency and plant survival rate are very low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The invention discloses a method for cultivating polyploid Luo Han Guo plants. The stem tip of the sterile test-tube plantlet of the female Luo Han Guo plant is taken, the epidermis cells of the leaves are scratched, the front side faces up, and the stem tips are flatly inoculated in MS induction liquid medium for cultivation, so as to obtain Luo Han Guo cluster buds.

[0043] The shoot tip of Luo Han Guo is selected as the culture part, which has many and fast buds, and has a good culture effect, overcoming the gender identification and long breeding cycle of the offspring obtained from the doubling of unpollinated ovaries, immature embryos, seeds (seedlings), etc. insufficient.

[0044] In the method for cultivating polyploid Luo Han Guo plants, plant growth regulators TDZ, gibberellins and colchicine are added to the MS induction liquid medium, and the addition amounts are respectively: 0.5 mg / L and 1.2 mg / L and 0.8g / L, wherein, each leaf obtained 20-25 buds.

[004...

Embodiment 2

[0058] A method for cultivating polyploid grosvenoria grosvenori plants. The stem section with axillary buds of the aseptic test-tube plantlet of grosvenoria grosvenorii female plant is taken, the epidermal cells of the stem section are scratched, the front side is facing upward, and the stem section is inoculated in MS induction liquid medium for cultivation, and the obtained Luo Han Guo bush buds.

[0059] The stem section of Luo Han Guo is selected as the culture part, which has many and fast buds and has a good culture effect. It overcomes the problems of sex identification and long breeding cycle for the offspring obtained from the doubling of unpollinated ovaries, immature embryos, seeds (seedlings), etc. insufficient.

[0060] In the cultivation method of polyploid Luo Han Guo plants, the MS induction liquid medium is added with plant growth regulator TDZ, gibberellin and colchicine, and the addition amounts are respectively: 1.5mg / L, 2.2mg / L L and 1.5g / L, wherein 22-2...

Embodiment 3

[0074]A method for cultivating polyploid grosvenoria plants. The young leaves of aseptic test-tube seedlings of grosvenoria grosvenori female plants are taken, treated with scratching of epidermal cells of the leaves, face up, and inoculated flatly in MS induction liquid medium for culture, so as to obtain grosvenoria grosvenori cluster buds.

[0075] The leaves of the aseptic test-tube seedlings of the female plant of Luo Han Guo were selected as the culture part, with many and fast germinations and good culture effects, overcoming the need for gender identification, The shortcoming of breeding cycle is long.

[0076] In the cultivation method of polyploid Luo Han Guo plants, the MS induction liquid medium is added with plant growth regulator TDZ, gibberellin and colchicine, and the addition amounts are respectively: 1.0mg / L, 1.0mg / L and 1.0g / L, wherein 24-30 buds were obtained per leaf.

[0077] TDZ is a new type of plant growth regulator with strong action. It has strong c...

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Abstract

The invention belongs to the field of plant genetic breeding and provides a cultivation method for a polyploidy siraitia grosvenorii plant. The cultivation method for the polyploidy siraitia grosvenorii plant comprises the following steps: cultivating blades or stems of a siraitia grosvenorii female / male plant by applying an MS induced liquid culture medium to obtain siraitia grosvenorii clumpy shoot so as to be used for preparing the polyploidy siraitia grosvenorii plant, wherein epidermis cell scratch treatment is carried out on the blades or stems of the siraitia grosvenorii female / male plant before cultivation. The cultivation method for the polyploidy siraitia grosvenorii plant has the advantages that the polyploidy siraitia grosvenorii plant with excellent maternal inheritable characters can be rapidly and efficiently obtained, cost is low, efficiency is high, a variety is good, chromosome doubling of the siraitia grosvenorii female / male plant is realized, the obtained polyploidy siraitia grosvenorii plant is a prepared tetraploid usually and can be hybridized with a diploid siraitia grosvenorii male / female plant to obtain a triploid seedless variety, so that the cultivation method for the polyploidy siraitia grosvenorii plant has an important agricultural and medicinal guiding significance.

Description

technical field [0001] The invention belongs to the field of plant genetics and breeding, and in particular relates to a method for cultivating polyploid monk fruit plants. Background technique [0002] Luo Han Guo is the first batch of precious Chinese herbal medicines for both medicine and food announced by the Ministry of Health. The fruit has high nutritional value, rich in vitamin C, glycosides, fructose, glucose, protein, lipids, etc. Mogroside is a low-calorie, high A natural sweetener with the highest sweetness in the world, low calorie and non-toxic, it can be eaten by diabetics and obese patients. Luo Han Guo is sweet, sour, and cool in nature. It has the functions of clearing heat and cooling blood, promoting body fluid and relieving cough, smoothing intestines and detoxifying, rejuvenating skin and beautifying skin, moistening lungs and reducing phlegm. It is a famous authentic medicinal material in Guangxi. The output of Luo Han Guo in Guangxi accounts for more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李刚王晓峰屈啸声唐美琼缪剑华马小军韦坤华周琼李正文
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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