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Tissue culture rapid propagation method for cucurbits

A tissue culture rapid propagation and gourd technology, applied in the field of plant tissue culture, can solve the problems of long cycle and low efficiency

Inactive Publication Date: 2019-04-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the object of the present invention is to provide a method for rapid propagation of gourd through tissue culture, which can overcome the disadvantages of conventional breeding, such as long and low efficiency, and provide a method basis for cell engineering and transgenic improved breeding of gourd; and the operation is simple, The production cost is low, the application value is high, and the seedlings emerge quickly and quickly, providing a reference for the large-scale production of high-quality gourd rootstock seedlings

Method used

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  • Tissue culture rapid propagation method for cucurbits
  • Tissue culture rapid propagation method for cucurbits
  • Tissue culture rapid propagation method for cucurbits

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Materials: Gourd FR seeds, a special rootstock for strong watermelon, were purchased from Hunan Xuefeng Seed Industry Co., Ltd.

[0039] Select gourd seeds with a smooth surface and no cracks, peel off the seed shells, and leave intact seed kernels. Disinfect them with 75% alcohol for 90 seconds in an ultra-clean workbench, rinse them with sterile water for 3 to 5 times, and then use 10% alcohol to clean them. Disinfect with sodium hypochlorite for 10 minutes, rinse with sterile water for 3 to 5 times, blot the excess liquid with sterile filter paper, and place it in the solid culture medium of sterile seedlings (MS+0.6% agar+3.0% sucrose, pH5.8). 8 seeds were bottled, and the seeds were germinated and cultured in the dark for 3 days, then switched to light culture, the light time was 14h / d, the light intensity was 2000lx, and the temperature during the whole culture period was 26°C.

[0040] When the aseptic seedlings are 6 days old, and the cotyledon is slightly expan...

Embodiment 2

[0047] The clustered shoots of 2~3cm in Example 1 were cut off, transferred to the rooting medium (1 / 2MS+IBA0.1~0.3mg / L+0.6% agar+3.0% sucrose, pH5.8), each Treat and inoculate 30 explants, set 3 repetitions, light time 14h / d, light intensity 2000lx, temperature during the whole culture period is 26°C, count rooting rate and root growth state, the results are shown in Table 2:

[0048] Table 2 Effects of different media on root differentiation

[0049]

[0050] Adding 0.1~0.3mg / L IBA to 1 / 2MS medium can take root, and the rooting rate and state are the best on 1 / 2MS+IBA 0.2mg / L rooting medium, up to 95%, and the root system is developed , stout and large in number.

[0051] The invention provides a method for rapid propagation of gourd tissue culture, which can obtain a large number of gourd seedlings with the same genetic traits within 2 months, and the multiplication coefficient can reach 2.27, which overcomes the disadvantages of long and low efficiency of conventional ...

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Abstract

The invention provides a tissue culture rapid propagation method for cucurbits, and relates to the technical field of tissue culturing for plants. The method comprises the following steps that 1), cucurbit seeds without hulls are disinfected and inoculated to the surface of an aseptic seedling solid culture medium for aseptic seedling culturing, and aseptic seedlings are obtained; 2), cotyledonarynodes of the aseptic seedlings are peeled and inoculated in a multiple shoot induction culture medium for induction culturing, and multiple shoots are obtained; 3), the multiple shoots are separatedand inoculated in a rooting culture medium for rooting culture, and tissue culture rapid propagation seedlings of cucurbits are obtained. By utilizing the method, within two months, a large number ofgrown cucurbit seedlings with consistent inheritable characters are obtained; the highest multiplication coefficient can reach 2.27, and the highest rooting rate can reach 95%. The method provides method basis for cell engineering of the cucurbits and transgenosis breeding improvement and provides reference for large-scale production of high-quality cucurbit stock seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of gourd. Background technique [0002] Plant tissue culture is a fast and effective bioengineering breeding method, which is not limited by seasons and regions. Plant tissue culture is also called in vitro culture. Plant tissues and organs are isolated under sterile conditions, and cultured in vitro under artificial control conditions to obtain mature callus, and regenerate complete plants after redifferentiation. From the totipotency of cells, it can be known that any tissue and organ of a plant can grow into a complete plant under certain conditions. However, there are many differences in the totipotency of these isolated tissue cells, and even different parts of the same tissue organ are not necessarily the same. In addition, factors such as genotype, seedling age, medium, and culture conditions cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 缪倩程兆榜任春梅季英华杨柳
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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