665results about How to "Improve seed setting rate" patented technology

The present invention discloses a paddy field rail duck raising technology. Rice, insect, duckweed and duck constituting one biological chain are made to control each other; the time and the space for the four kinds of organisms to constitute the complex colony are controlled based on the rice growth rule and the symbiotic biological environment for rice and duck is improved to realize the pollution-less rice plantation and duck raising. By stimulating natural biological system, the duck raising in paddy field can control the harmful disease, pest and weed, reduce pesticide amount, produce green food material and increase rice field output value.

The invention relates to a method for cultivating seedlings of oil peonies, and belongs to the field of agricultural seedling cultivation. The method comprises the following steps: selecting a nursery; performing weeding in advance; performing deep ploughing and tedding; performing soil preparation and making furrow ridges; harvesting seeds; processing the harvested seeds; sorting the processed seeds; processing the sorted seeds before cultivation; sowing the processed seeds; managing seedlings; managing nursery stocks. The characteristics of soil are changed by the method of combining farm manure with a chemical fertilizer. The seeds for sowing are harvested at the best harvesting time; the seeds to be cultivated are processed by gibberellin, ethyl alcohol, sodium p(dimethylamino) benzenediazo sulfonate solution, carbendazim and plant ash, and a culture solution is generated during the cultivation of onions, so that the seed dormancy is broken, the germinating time is advanced, and the germination rate is increased. Diseases are prevented during cultivation, and different medicaments are sprayed in different seasons so as to prevent diseases, so that the seedling survival percent is increased. After being cultivated by the method disclosed by the invention, the nursery stocks are thick in plants, the seedling survival percent is high, the quality is greatly improved, after the nursery stocks are planted, the seed setting rate is high, and the nursery stocks cultivated by the method disclosed by the invention are good varieties for the oil peonies.

The invention provides an acidic soil conditioner, and a preparation method and application thereof. The acidic soil conditioner comprises the following components in part by weight: 4 to 8 parts of composite bacterial agent, 80 to 90 parts of acid adjusting factor, 3 to 7 parts of chelate and 2 to 6 parts of organic matter. The acidic soil conditioner adjusts the pH value of soil, promotes formation of soil aggregate structures, improves the physical property of the soil, creates a good ecological environment for the growth of plants, increases organic matters, calcium, magnesium and silicon and the activities thereof, promotes healthy growth of crops, enhances stress resistance and prevents diseases and pests. By adoption of the composite bacterial agent, the constitution of a microflora of the soil can be improved, the microbial activity of the rhizosphere of the crops is enhanced, mineralization of organic pollutants is accelerated, growth of the plants is promoted, and absorption of degradation products and bioremediation of the polluted soil are accelerated. The acidic soil conditioner has no toxicity, residues and pollution, accords with the development tendency of organic pollution-free agriculture and has great popularization and application values.

The invention relates to a slow release / controlled release fertilizer for regulating and controlling crop nutrition all the time and preparation method thereof, wherein the fertilizer comprises a framework integument and exterior film using three proportions of nitrogen, phosphor and potassium as the core, and different integument material, integument processes and different emancipation systems are employed, the fertilizer also comprises a releasing bundle layer for controlling the fertilizer, addition agent, binding agent with adhesive action, amendment agent, solubilizing agent and active agent.

The invention discloses an oil peony high-yield cultivation method and belongs to the technical field of agriculture. The method includes the steps of land selection and soil preparation, sowing and seedling cultivation, transplanting, field management and disease and pest prevention. The method is easy to operate and suitable for large-scale application and popularization. By the adoption of reasonable fertilizer nutrition matching, nutrition requirements of oil peony growth in all stages are met, so that cultivated oil peonies are high in yield, high in seed setting rate and high in oil content. Natural traditional Chinese medicine components are adopted to serve as sterilization agents to process seeds, invasion of diseases and pests is prevented, the seedling emergence rate is increased in the seedling cultivation process, and disease resistance in the seedling cultivation process is improved. Besides, traditional Chinese medicine components are selected and used to serve as disease-resistant agents in the cultivation process, use of chemical pesticide components is reduced, environmental pollution is reduced, and the plant safety coefficient is obviously increased.

The invention provides a bio-control bacterium foliage fertilizer, and preparation and application thereof. The bio-control bacterium foliage fertilizer is prepared from the following materials in parts by weight: 1-3 parts of core bio-control bacteria, 35-85 parts of biochemical fulvic acid potassium, 10-50 parts of active SiO2, 2-6 parts of an enzyme activity substance, and 2-6 parts of a plant surfactant. The core bio-control bacteria contain bio-control promoting bacteria and protein decomposing bacteria; the bio-control promoting bacteria comprise bacillus subtilis and trichoderma harzianum. The core bacteria in the bio-control bacteria foliage fertilizer can rapidly grow on the surface of a plant in a field planting manner and form a 'bio-control membrane' so as to prevent infection of pathogenic bacteria at the surface of the plant; the substances, such as antibiotics and phytohormone, are generated in the processes of interaction with the pathogenic bacteria and hyperparasitism, and a plurality of diseases of crops can be prevented, resisted or directly killed; the bio-control bacteria foliage fertilizer enables the crops to have solid plant cell wall tissue, so that the ability of plant epidermis for directly resisting incursion of the pathogenic bacteria and insect pests is enhanced, and healthy growth of the crops is facilitated; the target of using a little of pesticide or not is achieved, and the environmental pollution is alleviated. Thus, the yield and the quality of the crops are improved.

The invention relates to a fertilizer formula and further relates to an application method of the fertilizer. A selenium-rich organic / inorganic fertilizer for producing selenium-rick rice is prepared by mixing selenites, nitrogenous fertilizers, phosphate fertilizers, potash fertilizers, rotten organic fertilizers and silicon fertilizers according to a certain ratio and pelletizing the mixture. The selenium-rich organic / inorganic fertilizer for producing the selenium-rick rice comprises the following components in parts by weight: 0.004-0.010 parts of the selenites, 15-33 parts of the nitrogenous fertilizers, 12-24 parts of the phosphate fertilizers, 10-17 parts of the potash fertilizers, 30-50 parts of the rotten organic fertilizers and 5-8 parts of the silicon fertilizers. The fertilizer not only includes selenium element but also includes other nutrition enhancing matters requested by crop growth. As the fertilizer can be used for supplying the selenium element and the other nutrition enhancing matters requested in growth for crops simultaneously, the application of the fertilizer is good for the crop growth, so that the absorption rate and the conversion rate of selenium are improved, and the selenium-rick rice reaching a selenium-rick rice standard can be produced by applying less selenium.

The invention discloses a method for cultivating organic selenium-enriched rice. The method is characterized by comprising the following steps of: respectively preparing selenium-enriched seedling cultivating matrixes and selenium-enriched organic and inorganic special fertilizers by taking selenium-containing flax cakes as a selenium source, utilizing the selenium-enriched seedling cultivating matrixes in the seedling cultivation of rice, and respectively applying the selenium-enriched organic and inorganic special fertilizers as a base fertilizer and additional fertilizer before transplanting seedlings of the rice and before the farmland rest at the last phase of tillering of the rice. According to the method for cultivating the organic selenium-enriched rice, disclosed by the invention, conventional methods in which selenium-containing solution or selenium-containing leaf surface fertilizer is sprayed at a flowering phase and a seed phase of rice in the current selenium-enriched rice production or the selenium-containing solution is added in a rice processing procedure are changed, unsafe factors in the production of the selenium-enriched rice are avoided, and the production ofthe selenium-enriched rice is economical, efficient and pollution-free.

A Fagopyrum esculentum culture method comprises the steps of 4-5 days before sowing, sun-drying seeds for 2-3 days; selecting seeds to remove small, impure and hollow seeds; soaking seeds with 40 DEG C warm water for 10 min, or soaking seeds or sealing seeds with 5-10% plant ash leachate for 1-2 days, to improve germination rate; applying base fertilizers before sowing; sowing when ground temperature reaches 10-15 DEG C, at amount of 60,000-70,000 plants per 667 square metres; performing first inter-tillage when seedling height reaches 10 cm; performing inter-tillage for 2-3 times during whole development period; and harvesting when 2 / 3 of grains of whole plant turn black and mature.

The invention belongs to the technical field of fertilizer application, and particularly relates to a method for application of organic fertilizer substituting chemical fertilizer in a quantitative manner. The method comprises the following steps of: S1, according to the crop growth environment, estimating the obtainable yield of a certain field under the best nutrient management measures according to the average yield of the crop in the past several years; S2, respectively calculating the agronomic efficiency of nitrogen fertilizer and the total quantity of nitrogen required to achieve the obtainable yield; S3, according to principle that the nitrogen nutrient of the organic fertilizer substitutes the nitrogen nutrient of the chemical fertilizer in a quantitative manner, respectively calculating the recommended application quantity of the organic fertilizer and the quantity of the nitrogen fertilizer substituted by the organic fertilizer; and S4, performing fertilizer application according to the recommended quantity of the organic fertilizer to the field, wherein the recommended quantity of the organic fertilizer is obtained through calculation. The method disclosed by the invention has the advantages of simplification, quantification, rationalization and the like. By only evaluating the crop growth environment and the soil fertility status, and using the key parameters such as the obtainable yield, nitrogen fertilizer reaction indexes and organic fertilizer substitution indexes, the total application quantity of the nitrogen fertilizer and the organic fertilizer can be quickly and accurately estimated, and the crop yield and the nitrogen use efficiency are effectively increased.

The invention discloses a paddy rice cultivation method of double-season mechanized straw returning to field. The method comprises following steps: mechanized crushing of straw returning to field, returning straw to fields, field growing management after returning straw to fields and disease and pest diseases control.The paddy rice cultivation method of double-season mechanized straw returning to field has following beneficial effects: through an original-position full-amount returning mode, resources of double-season early rice and late rice can be reasonably utilized; straw is circularly utilized on the spot and workload is decreased; in addition, environmental contamination and hardened and impervious soil caused by burning of straw are avoided so that soil is improved and fertility betterment is achieved reasonably and soil fertility is improved; fertilizer applying amount is reduced and soil quality is improved; and the method is beneficial for stable and high-yield of double-season early rice and late rice.

The invention relates to a double-season sunflower planting method which comprises that two-season sunflowers are planted in a double-season sunflower planting area of a Bohai Sea coastal saline area worked out through years of meteorological data and a geographic information systems method in spring and in summer; and the method comprises a first season sunflower spring planting and a second season sunflower summer planting. The whole process is divided into five parts, i.e. land preparation and fertilization, timely sowing, sowing method, field management and harvest. By fully satisfying the heat need required by the two-season sunflower crops, the invention reduces insect pest and the chemical use times, improves the land utilization rate, constructs the double-season sunflower planting method by accumulating temperature in the area suitable for growth, and realizes the high yield, the high quality and the high-efficiency plantating of the sunflower crops.

The invention discloses a castration method for rice hyberdization, belongs to the technical field of crop breeding and particularly relates to a new castration method for rice hyberdization based on spikelet clipping and water spraying. The castration method for rice hyberdization comprises the following steps: 1, determining reasonable sowing time; 2, selecting rice plants; 3, pruning spikes of whole plants; 4, performing castration based on spikelet clipping and water spraying; and 5, performing pollination timely and efficiently. According to the castration method disclosed by the invention, the problems of high labor intensity, incomplete castration and hyberdization failure due to pistil damage in the past rice hyberdization work are solved, and the technology used in the castration method has the advantages of labor and cost economy, simplicity and convenience in operation, high hyberdization success rate, economy, environment friendliness and the like, and is highly popular among scientific research workers on breeding, and has a wide application prospect.

The invention discloses long-acting slow release organic fertilizer which is prepared by raw materials in parts by weight as follows: 4-5 parts of activated carbon, 10-15 parts of edible starch pulp, 5-6 parts of zeolite powder, 7-8 parts of medical stone powder, 6-8 parts of gelidium amansii, 4-5 parts of potassium sulfate, 35-45 parts of animal waste, 12-18 parts of spirulina powder, 1-2 parts of talcum powder, 3-4 parts of ground phosphate rock, 5-6 parts of ammonium sulfate, 2-3 parts of urea, 3-4 parts of sodium sulfate, 5-6 parts of biochemical fulvic acid, 20-30 parts of a soil conditioner and appropriate water. The long-acting slow release organic fertilizer adopts a double-envelope structure comprising a molten urea layer and the talcum powder, by means of slow release of the urea and high adsorbability of the talcum powder, release of fertilizer nutrients can be controlled effectively, waste caused by the fact that a large quantity of fertilizer components cannot be effectively absorbed by plants is prevented, meanwhile, the added raw materials such as the gelidium amansii, the spirulina powder and the like contain large quantities of trace elements such as iron, calcium, magnesium and the like required by the plants, and healthy growth of the plants is facilitated.

The invention belongs to the field of heterosis application and in particular relates to a method for producing rice hybrid Guangliangyou 206. According to the invention, a new combination of three-line early rice is produced by hybridizing three-line male sterile line Guang 8S with restorer line D208, wherein the female parent Guang 8S is quoted from high-yield, high-quality and high-resistance three-line male sterile line selected and bred by teacher Yang Zhenyu; male parent restorer line D208 is introduced. The hybrid has the characteristics of high and stable yield, strong disease resistance and high biological yield.

The invention discloses a good-quality and high-yield seed production technique of a muskmelon, is applicable to seed production of the muskmelon, and relates to the technical field of plant cultivation. The good-quality and high-yield seed production technique comprises the following steps: grafting and growing seedlings, applying fertilizers and irrigating water, pruning, pollinating, castrating and rogueing, and carrying out seed collecting treatment. According to the good-quality and high-yield seed production technique, the nutrient absorption capability of roots of a melon is enhanced through adopting the step of grafting and growing seedlings, so as to meet the vegetative growth of plants in a melon seed production process, guarantee the nutrient supply of seed development and realize the aims of improving the yield and the quality of seeds; the step of pruning is carried out on three vines; 1-2 melons are set on each wine and 3-4 melons are set on each plant, so that the quantity of seed melons in a unit area can be effectively improved; meanwhile, the quantity of the seeds of the single melon is improved and the aim of improving yield of the seeds is realized; single-plant cross pollination is finished within 3 days and the nutrition supply of the plants is balanced; the fruit setting rate and the seed setting rate of the melon are improved. Compared with a conventional seed production technique, the good-quality seed production technique disclosed by the invention has the advantages that the yield of the seed in the unit area can be improved by more than 30% and the output capacity of the good-quality seeds is improved by more than 15%.

The invention relates to organic fertilizer and concretely relates to a method for preparing bio-organic fertilizer from walnut green seedcase. The method realizes walnut by-product utilization. The method comprises adding a professional starter into walnut green seedcase with water content of 70-80%, fully mixing the professional starter and the walnut green seedcase according to a weight ratio of 1: 1000, mixing the mixture and animal dung according to a weight ratio of 30-50%, putting the mixture into a fermentation tank, carrying out fermentation, wherein in fermentation, a turning process is carried out and after the temperature is reduced to 30 DEG C from 60-70 DEG C and the pile is stood for 10-20 days, adding 10% of a biological compound bacterium fluid into the fermented material and carrying out granulation to obtain the bio-organic fertilizer.

The invention relates to the field of fertilizers, and concretely relates to a multielement composite foliar fertilizer. The multielement composite foliar fertilizer is composed of the following substances in percent by mass: 10-20% of urea, 5-7% of diammonium phosphate, 3-5% of potassium sulfate, 0.05-0.15% of ferric sulfate, 0.02-0.04% of zinc sulfate, 0.03-0.05% of sodium molybdate, 0.1-0.3% of borax, 0.03-0.05% of magnesium sulfate, 0.07-0.09% of potassium chloride, 0.07-0.09% of iodoacetic acid, and 1-3% of a plant-growth regulator.

The invention discloses an improved fertilizer for saline and alkaline land and a preparation method of the improved fertilizer. The improved fertilizer for the saline and alkaline land is prepared from the following raw materials in parts by weight: 25-28 parts of maleic anhydride, 15-20 parts of citric acid, 20-30 parts of calcium superphosphate, 10-12 parts of a sodium ion adsorbent, 2-4 parts of a microbial agent, 25-32 parts of fulvic acid, 15-20 parts of a trace element fertilizer, 30-38 parts of a chicken manure fermentation fertilizer, 20-35 parts of peat, 3-5 parts of perlite, 5-8 parts of vermiculite and 20-25 parts of smashed straw particles. The invention further provides the preparation method of the improved fertilizer for the saline and alkaline land. According to the improved fertilizer for the saline and alkaline land, various soil improving measures can be synthesized, effects of reducing salt and alkali for the saline and alkaline land are good, the water content of the soil is high, the water retention property and the fertilizer retention property of the soil are good, besides, the survival rate of planted plants is high, and virtuous cycle of the soil of the saline and alkaline land is realized.

The invention provides a full set of dry-finishing direct-sowing rice cultivating technologies which can save labor, increase output, resist fall-down and improve soil and the land farming mud layer. As the dry-cultivation and the dry-finishing are adopted, the soil has good crumb structure, good hydroscopic property and good water permeability, which facilitates to ensure that the seedlings can tiller earlier and more at lower positions and the basic seedlings are sufficient; a high-yield seedling rack can be formed easily without transplanting and seedling mitigating and the mature period can be advanced for 7-10 days. By adopting the technology, the requirement of the rice for water in the middle and last period is looser, the maturing rate is high, the kernel is heavy and big and the land farming mud layer can be kept in an optimum state for ever.

The invention relates to a double-cropping planting method for oil sunflowers in coast heavy saline soil. The double-cropping planting method for the oil sunflowers in the coast heavy saline soil is characterized by comprising the following steps of: A, confirming a heat-suitable area and suitable sowing time for the oil sunflowers; B, building raised field-shallow pond reformed heavy saline soil; C, planting the oil sunflowers in raised fields in spring and summer and performing sea-ice water irrigation; and D, harvesting. The double-cropping planting method for the oil sunflowers in the coast heavy saline soil has the beneficial effects that a stable soil environment is provided to planting of the oil sunflowers by utilizing a reforming method of building raised fields-shallow ponds; water demand for the oil sunflower sowing time and a growth period is guaranteed through the sea-ice water irrigation, and therefore, the land utilization efficiency and the yield of the oil sunflowers are greatly increased, and the problems that land and water resources of the coast heavy saline soil are unused and the land production potential is low are solved.

The invention discloses a method for culturing oil peonies. The method comprises the following steps of (1) seedling plant processing, seedling plants are soaked in a 800-fold thiram solution for 15 min to 20 min, and then are dried to be planted; (2), culturing, culturing is carried out according to the seed spacing of 20*25 cm, in the culturing process, a shovel is inserted into the ground to drive open a gap, a seedling is put into the gap, and is slightly lifted to enable the rootstock portion to be 1 cm to 2 cm lower than the ground, and planting holes are sealed into ridges of 10 cm to 20 cm; (3), fertilization, in the first year of planting, fertilization is avoided, and from the second year of planting, fertilizer is applied twice every year; (4) pest control, at the early march, 10 to 15 kilograms of phoxim granules are applied to per mu; at the mid to late April, 600 to 800-fold liquid of 70 percent of thiophanate methyl is sprayed and applied before 7 days to 10 days of the flowering phase; from beginning of the last ten-day of May, 600 to 800- fold liquid of carbendazim or thiophanate methyl or chlorothalonil is sprayed and applied at intervals of 15 days to 20 days. According to the method, the seed setting rate of the oil peonies is improved, the method is large in seed setting amount and oil yield, and increasing of vegetable oil consumption per capita is met.

The invention relates to a breeding and cultivating method for a plant, in particular relates to a breeding and cultivating method for loose purple cauliflowers, and belongs to the technical field of plant cultivation. The breeding and cultivating method comprises the steps of hybridizing a loose purple cauliflower breeding material with a semi-compact purple cauliflower breeding material to obtain seeds F1, and selfing single strains with high comprehensive agronomic characters after the seeds F1 are sowed to obtain seeds F2; performing microspore culture on single loose purple cauliflowers through flower buds suitable for the microspore culture from the mononuclear edging stage to the double-core early stage to grow a large number of normal regenerated plants, selfing excellent DH plants of the loose purple cauliflowers to obtain a loose purple cauliflower excellent DH strain 03-12 serving as a recurrent parent which is hybridized with a cauliflower cytoplasmic male sterility source material CMS05-6, performing five generations of backcross transformation and selection to form a cytoplasmic male sterile line CMS08-2, and hybridizing the DH strain serving as a parent with the excellent cytoplasmic male sterile line CMS08-2.

The invention discloses a rice overgrowth-controlling, lodging-resistant and yield-increasing agent and an application thereof. The rice overgrowth-controlling, lodging-resistant and yield-increasing agent mainly comprises a plant growth regulator and a fertilizer, wherein the regulator is brassinolide and paclobutrazol or uniconazol, and the fertilizer is one or combination of more than two of substances containing N / P / K / B / Zn / Ca / Cu / Fe / Mn / Mo / Me / S / Cl / Si and other elements, such as urea, potassium dihydrogen phosphate, diammonium phosphate, calcium carbonate, potassium nitrate, silicon fertilizer, zinc sulfate, ferrous sulfate, potassium chloride, boric acid, borax, copper sulfate, ferrous sulfate, molybdenum fertilizer, magnesium sulfate, potassium sulfate, manganese sulfate and rare earth fertilizer. The rice overgrowth-controlling, lodging-resistant and yield-increasing agent has good safety and can effectively shorten the length between neck-panicle nodes and top second nodes of rice and enhance lodging resistance of rice; simultaneously, the rice overgrowth-controlling, lodging-resistant and yield-increasing agent can not affect safe dates of full heading and the grain number per spike of rice, prolong the service life of functional leaf of rice, improve the seed setting rate and the 1000-grain weight of rice and significantly affect the yield of rice. The rice overgrowth-controlling, lodging-resistant and yield-increasing agent is suitable for various types of rice, including conventional rice, hybrid rice and super hybrid rice, and has the advantages of low cost and high performance-price ratio.

The invention discloses a seedling growing and machine transplanting method of double cropping rice. The method comprises the following steps: early rice cultivation: making a seedbed on January to February, building a greenhouse, preparing a substrate, placing 20-25 hard seedling trays of (28*58*2.5) centimeters into each mu of field, sowing and performing seedling management; double cropping late rice cultivation: making a seedbed on April to May, preparing a substrate, placing 26-30 hard seedling trays of (28*58*2.5) centimeters into each mu of field, sowing and performing seedling management; machine transplanting and management of early rice; machine transplanting and management of double cropping late rice. According to the method, cured organic nutritional soil is taken as a substrate in seedling cultivation of early rice and late rice, so that sufficient nutritional components are provided for seedlings, and the environmental pollution is low; a sufficient amount of compound fertilizer is applied after machine transplanting of rice, a tiller fertilizer is applied and enclosed weeding is performed as early as possible, a jointing and flower promoting fertilizer is applied once again, prevention and control of banded sclerotial blight are performed, and harvesting is performed in time; specific to the difference in the seedling cultivation time of early rice and late rice, the fertilization and plant disease and insect pest prevention and control work of rice are performed, so that the maturing rate is increased.

The invention discloses special cotton fertilizer for regulating and controlling coastal saline-alkali land soil and belongs to the technical field of special fertilizers for crops. The special cotton fertilizer is prepared from the following raw materials in parts by weight: 300-450 parts of urea, 1-100 parts of diammonium phosphate, 50-120 parts of potassium dihydrogen phosphate, 100-200 parts of potassium sulfate, 50-150 parts of calcium humate, 1-50 parts of calcium-magnesium phosphate, 1-50 parts of magnesium sulfate, 1-10 parts of zinc sulfate and 1-10 parts of high-strength water retention agent. Compared with the prior art, the special cotton fertilizer has the advantages that due to the addition of water-soluble calcium humate, the special cotton fertilizer can be used for inhibiting salt and holding moisture; due to the addition of a proper volume of water retention agent, the special cotton fertilizer can be used for regulating and controlling water retention, increasing the fertilizer effect and increasing fertilizer utilization ratio; due to the addition of essential secondary / trace elements, the resilience resistance is enhanced, and the product yield and quality are improved.

The invention relates to an improved ultrasonic-assisted pollen mediated plant genetic transformation method, and aims to obviously increase the setting percentage of the plant fertilized by pollen subjected to ultrasonic treatment, thereby increasing the number of transformants obtained for each treatment. The method comprises the following steps: by using an agrobacterium Ti plasmid carrying an exogenous gene segment, colibacillus plasmid or any other DNA vector as a genetic donor and using plant pollen as a receptor, mixing the pollen and exogenous DNA in a 5-50% sucrose solution subjected to aeration and low-temperature treatment, and transferring the exogenous gene into the receptor pollen under the assisting action of ultrasonic; fertilizing the treated pollen onto the stigma of the plant, and harvesting when the grains become ripe; and in the subsequent growth season, sowing the harvested seeds which are obtained after the fertilization of the transformed pollen, screening the germinated seeds and seedlings, carrying out PCR (Polymerase Chain Reaction) amplification and Southern hybridization on the DNA of a seedling sample, and further determining the transformant. The method provided by the invention does not need tissue culture, does not have species or genotype dependency, and can shorten the genetic transformation breeding time and save the manpower and material resources.

The invention discloses a geopolymer injecting paste material and a preparing method and application thereof. The geopolymer injecting paste material is prepared from the components in proportion: 50-80 parts of coal ash, 16-50 parts of zeolite powder, 4-10 parts of clay, 5-15 parts of alkali excitant, 0.5-2 parts of accelerator, 3-8 parts of water glass and a water cement ratio is 0.75-1.5; the method comprises: (1) dissolving the alkali excitant in water to prepare an alkali excitant solution; (2) uniformly mixing the coal ash and the zeolite powder with other components to obtain a mixture; (3) mixing the alkali excitant solution with the mixture, and stirring to obtain the target geopolymer injecting paste material. The geopolymer binding material taking the coal ash, zeolite powder and clay as raw materials has the characteristics similar to but superior to those of cement, and can serve as an excellent injecting paste material, and has excellent effects on road foundation strengthening and antiseepage in engineering.

The invention provides a direct leaking stoppage fiber grouting material and a preparation method thereof. The fiber grouting material is prepared from the following substances in percentage by weight: 15 to 20 percent of Portland cement, 40 to 45 percent of sodium silicate, 5 to 10 percent of fly ash or mineral waste residues, 25 to 30 percent of water, 3 to 4 percent of basalt fibers and 1 to 2 percent of retarder. The preparation method comprises the following steps: equally dividing the fly ash or the mineral waste residues into two parts according to the mass ratio; uniformly mixing one part of the fly ash or the mineral waste residues with the basalt fibers, the Portland cement and the water to obtain a mixed solution I; uniformly mixing the other part of the fly ash or the mineral waste residues with the sodium silicate to obtain a mixed solution II; slowly pouring the mixed solution II into the mixed solution I for uniformly mixing to obtain the fiber grouting material. The fiber grouting material provided by the invention can meet the requirements on three properties, namely groutability, controllability and permeability resistance, also has the advantages of high setting rate, high long-term strength and the like at the same time, and has a broad application prospect in related fields such as goaf, roads and foundation treatment.