The invention discloses a highly-efficient genetic transformation method of the embryogenic
callus of rubber trees, which is characterized in that anters of rubber trees or inner integuments which are used as explant are proceeded
tissue culture, the culture is induced to produce embryogenic
callus of rubber trees, then the embryogenic
callus is proceeded successive transfer
enrichment culture, and the embryogenic callus after enrichment is utilized as
acceptor material to proceed genetic transformation. The successive transfer
enrichment culture is proceeded to resistant embryogenic calli which is achieved after the transformation, and the resistant embryogenic calli after the successive transfer
enrichment culture is induced the culture to produce
embryo which further becomes plants in the process of embryoid induction. The invention has the advantages of simple art procedure, excessively high
transformation efficiency and low cost, overcomes the serious shortcomings of extremely low percentage of immature embryos of the existing genetic transformation
system of rubber trees, and the like, resolves the disadvantages that the achievement of the materials of transformation
acceptor of rubber trees is easy to be restricted by natural conditions, such as seasons, and greatly reduces the transformation period, thereby the invention is an effective genetic transformation method of rubber trees and has great scientific value and application value.