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Producing method of repairable pigling pancreatic island cell

A technology of islet cells and production methods, applied in the field of biological cells, can solve problems such as complex serum components and poor culture effects, and achieve the effects of low immune antigenicity, easy mutagenesis, and low cost

Active Publication Date: 2007-02-28
张俊英
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the composition of serum is complex, and it is easy to cause poor culture effect

Method used

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  • Producing method of repairable pigling pancreatic island cell
  • Producing method of repairable pigling pancreatic island cell

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Embodiment Construction

[0039] Specific implementation plan

[0040] The present invention is further described as follows in combination with specific steps:

[0041] 1. Selection of heterogeneous islets:

[0042] 1. The characteristics of the islets of newborn piglets:

[0043](1) Both human and pig insulin molecules have an A chain and a B chain, both of which are composed of 51 amino acids. The A chain contains 21 amino acids, and the B chain contains 30 amino acids. And there is a disulfide chain connection between the A and B chains. The structure is very similar, the difference is only one amino acid. The 30th amino acid of human insulin B chain is threonine. The 30th amino acid of pig insulin B chain is alanine. (2) Porcine insulin has been used in clinical treatment of type I diabetes patients for more than ten years, which fully proves the safety and effectiveness of porcine insulin in humans. (3) Newborn pig islets are weakly immunogenic and have little rejection. (4) Porcine pancreatic islet ...

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Abstract

The invention relates the method for producing renovation pigling Langer-hans' insula cell. The method comprises the following steps: 1 choosing donator: choosing health pigling, at sterility opening abdomen, getting pancreatic, removing integument, vessel, connective tissue in Hank`s liquid at 4Deg.C; 2 separating and purifying Langer-hans' insula cell: separating Langer-hans' insula cell from external secretion tissue with finishing machine, centrifuging with Dextran non-sequence density gradient, getting Langer-hans' insula cell; 3 culturing Langer-hans' insula cell: carrying out CO2 culturing with non-serum microballoon carrier, using RPMI-1640 culture liquid, adding fibrin, packaging the Langer-hans' insula cell in the APA biological film to form microsphere, at 37Deg.C, culturing in the CO2 culture box, changing culture liquid every 48 hours, and getting the product.

Description

Technical field [0001] The invention relates to the technical field of biological cells, in particular to a production method for repairing piglet pancreatic islet cells, which can be used clinically in the transplantation of pancreatic islets in diabetic patients. Background technique [0002] Diabetes is a global disease that endangers human health. The morbidity rate is 3-5%; the mortality rate is second only to cardiovascular disease and tumors. It is predicted that by 2025, there will be 300 million people with diabetes worldwide. Among them, type I diabetes patients (IDDM) will account for 5-10%; these people will depend on exogenous insulin for life. Long-term injection of insulin can cause local skin redness, swelling, itching, induration, infection, subcutaneous fat atrophy or fibrosis. Daily injection of insulin not only brings inconvenience and pain to patients, but more importantly, the application of insulin can only control hyperglycemia and cannot prevent its chron...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/071
CPCC12N5/0676
Inventor 张俊英王斌王章存范清堂
Owner 张俊英
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