210 results about "Plant genetics" patented technology

The invention relates to an improved ultrasonic-assisted pollen mediated plant genetic transformation method, and aims to obviously increase the setting percentage of the plant fertilized by pollen subjected to ultrasonic treatment, thereby increasing the number of transformants obtained for each treatment. The method comprises the following steps: by using an agrobacterium Ti plasmid carrying an exogenous gene segment, colibacillus plasmid or any other DNA vector as a genetic donor and using plant pollen as a receptor, mixing the pollen and exogenous DNA in a 5-50% sucrose solution subjected to aeration and low-temperature treatment, and transferring the exogenous gene into the receptor pollen under the assisting action of ultrasonic; fertilizing the treated pollen onto the stigma of the plant, and harvesting when the grains become ripe; and in the subsequent growth season, sowing the harvested seeds which are obtained after the fertilization of the transformed pollen, screening the germinated seeds and seedlings, carrying out PCR (Polymerase Chain Reaction) amplification and Southern hybridization on the DNA of a seedling sample, and further determining the transformant. The method provided by the invention does not need tissue culture, does not have species or genotype dependency, and can shorten the genetic transformation breeding time and save the manpower and material resources.