41results about How to "Avoid Biosafety Concerns" patented technology

The invention belongs to the technical field of plant biology and particularly relates to separation, function identification and application of a rice anther specific expression promoter. The disclosed promoter is specifically expressed in a plant anther and has good application prospects in the field of plant genetic modification.

The invention discloses a pollen-specific promoter and an expression vector thereof. Experiments show that the promoter can drive efficient and specific expression of a target gene in the pollen of transgenic rice. The promoter of the invention can be used for functional analysis and identification of genes related to growth and development of plant pollen, as well as creation of plant male sterile lines, prevention of plant transgenic drift, and the like. Especially, the promoter has an important application value in creation of male sterile lines of rice and other gramineous crops and utilization of heterozygous advantages.

The invention provides a method for efficiently detecting 15 kinds of bile acid in blood. In the method, serum is directly preserved on a filter paper scrip, and through an efficient extraction process, extracted bile acid is directly injected on a liquid chromatography-mass spectroscopy; 15 kinds of the bile acid in blood can be efficiently detected in a high-throughput and high-repeatability mode, and the method has the wide application value.

The invention discloses a biological mothballing system suitable for synechococcus elongatus, and a construction method and application of the biological mothballing system. The construction method comprises the steps that a virulent gene sepT2, an antitoxic gene sepA2 and an iron-deficiency inducible promoter PisiAB are obtained from the synechococcus elongatus 7942, and a promoter PpsbA2 is obtained from synechocystis 6803; a terminator Trbcl is obtained from integrative plasmid pBA3031 through amplification; pBR322 is taken as a carrier, the virulent gene sepT2 is expressed with the iron-deficiency inducible promoter PisiAB, and the terminator Trbcl is used for terminating transcription; and the antitoxic gene sepA2 is expressed with the promoter PpsbA2, and plasmid is constructed and transferred into the synechococcus elongatus. According to the system, the synechococcus elongatus grows normally under the non-inducing situation and rapidly dies after being induced. The important theoretical and practical significance for development and optimization of the biological mothballing system of the synechococcus elongatus is achieved, and reference is also provided for solving the bio-safety problem of other cyanobacteria.

The invention discloses a method for acquiring gene editing sheep by RNA-mediated specific FGF5 gene knockout and special sgRNA for the method. The sgRNA provided by the invention can realize specific targeted modification of sheep FGF5 gene, is RNA shown in the second to the 21st nucleotides from the sequence 4 to 5' end tail of a sequence table or RNA with the second to the 21st nucleotides from the sequence 4 to 5' tail end of the sequence table. The invention provides an effectively technical tool for sheep functional gene researches.

The invention discloses a series of plasmid vector for building an overexpression stable system on the basis of transposase and application thereof. The plasmid vector is a double-chain annular plasmid containing an IRDR-L-IRDR-R kit; the IRDR-L-IRDR-R kit comprises an IRDR-L sequence, a promotor, a tag sequence, an attR1 sequence, a reverse selective tag gene sequence ccdB, an attR2sequence, a screening gene sequence and an IRDR-R sequence; the tag is an HA tag, an Myc tag, a Flag tag or a V5 tag. The plasmid vector has a Sleeping Beauty transposon tail end reverse repeated sequence and a Gateway Cassette sequence; the overexpression stable system can be fast and efficiently built on the basis of the transposase method; various different tag sequences Tag and screening genes GFP exist, so that the conventional requirement of expressing the target gene in eukaryotic cells can be met.

The invention discloses an antigen for detecting a sheep echinococcosis antibody and a preparation method of an agar diffusion plate, and belongs to the technical field of biological detection. The preparation method of the antigen comprises the following steps: performing enzyme digestion connection on a sheep echinococcosis EG95 protein nucleic acid sequence, and constructing the sheep echinococcosis EG95 protein nucleic acid sequence on a plasmid vector; then performing converting into escherichia coli, constructing an expression strain, performing IPTG induced expression, and performing crushing to obtain an inclusion body; and performing washing, cracking, centrifuging, purifying, dialyzing, concentrating, freeze-drying, and diluting with PBS. The preparation method of the agar diffusion plate comprises the following steps: adding water and PBS into agarose, performing heating, adding polyethylene glycol 6000, MES and sodium chloride, performing cooling, performing punching, and sealing the bottom. The antigen and the agar diffusion plate provided by the invention have the advantages of high sensitivity, strong specificity and strong universality, have good biosafety, are easyfor large-scale production, are especially suitable for clinical detection of sheep serum by grassroots veterinarians, and have wide popularization and application value.

The invention belongs to the technical field of plant biology and particularly relates to separation, function identification and application of a plant anther specific-expression promoter. The promoter is specifically expressed in plant anther, so that the promoter is excellent in application prospect in the field of plant transgenosis.

The invention provides a self-heating amplification detection device. The self-heating amplification detection device comprises a reaction structure, a detection structure, an intermediate structure, a self-heating structure and a sealing structure. The self-heating amplification detection device can realize self-heating, can perform amplification reaction detection on different samples, and can also improve the safety performance of the device in the reaction process. The invention further provides a preparation method and a use method of the detection device, and the beneficial effects are as described above. The invention further provides a designed nucleic acid detection system, the nucleic acid detection system comprises an extraction device and a detection device, the RPA technology, closed nucleic acid extraction equipment and a self-heating-dependent colloidal gold test card are combined together, so that an extraction reaction system is completely isolated from the external environment, the biological safety problem can be effectively avoided while the detection time is saved by means of closed normal-temperature amplification, nucleic acid detection can be completely separated from large-scale instruments and equipment, and the on-site detection target of sampling while detecting is realized.

The invention belongs to the technical field of plant biology and particularly relates to separation, function identification and application of a rice anther specific expression promoter. The disclosed promoter is specifically expressed in a plant anther and has good application prospects in the field of plant genetic modification.

The invention discloses an unmanned weighing system and method. The unmanned weighing system and method are used for realizing automatic weighing of large-scale live pigs. The unmanned weighing systemcomprises a weighing end and a server end, and the weighing end and the server end are connected by a network; and the weighing end comprises an industrial control computer, a weighing unit, an alarmunit and a communication control box, the weighing unit is connected with a weight calculation unit of the industrial control computer, the alarm unit is connected to the communication control box, and the communication control box is connected with the industrial control computer. According to the unmanned weighing system and method, the whole process of weighing can be got through, the functions of automatic scheduling, automatic trading and the like are realized, and the efficiency of livestock product sales is improved.

The present invention belongs to the field of plant biotechnology, and particularly relates to separation, functional identification and applications of a plant anther-specific expression promoter. According to the present invention, the promoter is specifically expressed in the plant anther, and provides good application prospects in the plant transgene field.

The invention belongs to the technical field of plant biology, discloses identification and application of a plant anther specific expression promoter pTaASG005 and particularly relates to separation, function identification and application of the plant anther specific expression promoter. The promoter is specifically expressed in plant anthers, particularly in anthers of pollen in a uninucleate stage, a binucleate stage and a trinucleate stage. The plant anther specific expression promoter pTaASG005 has a promising application prospect in the field of plant transgene.

The invention relates to a hydrothorax and ascites sedimentation bottle. The bottle comprises a storage part for accommodating hydrothorax and ascites, the storage part is provided with an internal cavity for accommodating hydrothorax and ascites, a liquid inlet for loading hydrothorax and ascites and a sealing piece for detachably sealing the liquid inlet are arranged at the upper part of the storage part, the bottom of the storage part is provided with a sample discharge port for discharging target cells and a control piece for controlling the sample discharge port to be opened / closed, a liquid outlet for discharging supernatant and an adjusting part for controlling the opening / closing of the liquid outlet are arranged on the side surface of the lower part of the storage part, the liquidinlet, and the sample discharge port and the liquid outlet are respectively communicated with the internal cavity. The sedimentation bottle is simple and compact in structure, facilitates collection,storage and transfer of hydrothorax and ascites, can conveniently and safely treat supernatant while fully retaining cell suspension and / or precipitate, and prevents the biosafety problem caused by random discharge.

The invention discloses a promoter with anther tissue specificity. The promoter with the anther tissue specificity is as follows (a) or (b) or (c): (a), 3'end contains at least 1-1698 nucleotide sequences of a sequence 1 in a sequence table, beginning at the 1698 position of sequence 1 and extending to the 3' end of sequence 1 in accordance with the nucleotide sequence of the sequence 1, expandingto the 3' end of the sequence 1 according to the nucleotide sequence of the sequence 1, and any DNA fragment of length from 1698 to 2205bp is obtained; and a DNA molecule has a promoter function; (b)a DNA fragment is provided with a defined identity of 75% or more than 75% of the nucleotide sequence limited by (a), and provided with the promoter function; and (c) a DNA fragment hybridizes with the nucleotide sequence limited by (a) or (b) under strict conditions and is provided with the promoter function. The promoter can be used for the specific expression of exogenous genes in anthers andthe creation of male sterile and restorer lines, and a wide application prospect is achieved.

The present invention belongs to the field of plant biotechnology, and particularly relates to separation, functional identification and applications of a plant anther-specific expression promoter. According to the present invention, the promoter is specifically expressed in the plant anther, and provides good application prospects in the plant transgene field.

The invention belongs to the technical field of plant biology and particularly relates to isolation, functional identification and application of a promoter pTaASG019 featuring plant anther-specific expression. The promoter featuring plant anther-specific expression has promising application prospect in the field of plant transgenosis.

The present invention belongs to the field of plant biotechnology, and particularly relates to separation, functional identification and applications of a plant anther-specific expression promoter. According to the present invention, the promoter is specifically expressed in the plant anther, and provides good application prospects in the plant transgene field.

The invention provides a controllable assembly and disassembly supramolecule and a preparation method and application thereof. The controllable assembly and disassembly supramolecule has a structure as shown in a formula I. The controllable assembly and disassembly supramolecule provided by the invention can be used for drug delivery of a living body, can be stored for a long time at-20 DEG C, and solves the biosafety problem of polypeptide supramolecular assemblies in a current drug delivery system.

The present invention belongs to the field of plant biotechnology, and particularly relates to separation, functional identification and applications of a plant anther-specific expression promoter. According to the present invention, the promoter is specifically expressed in the plant anther, and provides good application prospects in the plant transgene field.

The present invention belongs to the field of plant biotechnology, and particularly relates to separation, functional identification and applications of a plant anther-specific expression promoter. According to the present invention, the promoter is specifically expressed in the plant anther, and provides good application prospects in the plant transgene field.

The invention discloses a method for acquiring gene editing sheep by RNA-mediated specific double-gene knockout and special sgRNA for the method. The sgRNA combination consists of sgRNAMSTN-1 and sgRNAFGF5-1, wherein sgRNAMSTN-1 is sgRNA which can realize specific targeted modification of sheep MSTN gene and is RNA shown in the second to 21st nucleotides in a sequence 6 or RNA with the second to 21st nucleotides of the sequence 6; sgRNAFGF5-1 is sgRNA which can realize specific targeted modification of sheep FGF5 gene and is RNA shown in the second to the 21st nucleotides in a sequence 8 or RNA with the second to 21st nucleotides of the sequence 8. According to the method for acquiring gene editing sheep by RNA-mediated specific double-gene knockout and special sgRNA for the method, the CRISPR / Cas9 genome editing technology and the micro-injection technology are combined, so that the sheep targeting efficiency is higher and more accurate, sheep double-gene knockout is realized for the first time in the generation, improvement on sheep meat production and wool production is greatly promoted, and a larger space and a more effective technical tool are provided for breeding of new sheep varieties.

The invention discloses a sample target processing system of a matrix-assisted laser desorption ionization time-of-flight mass spectrometer. The sample target processing system comprises a vacuumizingsystem, a vacuum sample injection chamber provided with a cabin door, and a vacuum drying chamber used for drying a sample target to be tested, wherein a moving part for bearing a sample target groove is arranged in the vacuum sample injection chamber, and the sample target groove is used for bearing a to-be-detected sample target; a transition chamber is arranged at the sample inlet of the vacuum sample injection chamber, and is used for enabling the sample target groove, the sample inlet and the cabin door to form a closed cavity in an enclosing manner when the sample target groove moves tothe sample inlet; an exhaust filter is arranged in a vacuumizing air path of the vacuum drying chamber and is used for filtering harmful impurities in the vacuum drying chamber; and the sample inletis communicated with the atmosphere through a valve. The method has the advantages that mass spectrometric detection can be completed without waiting for the drying of the sample target, so the working steps of mass spectrometric detection are greatly shortened, and meanwhile, the biosafety problem in the drying process is avoided.

The present invention belongs to the field of plant biotechnology, and particularly relates to separation, functional identification and applications of a plant anther-specific expression promoter TaASG046. According to the present invention, the promoter is specifically expressed in the plant anther, and provides good application prospects in the plant transgene field.