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Controllable assembly and disassembly supramolecule and preparation method and application thereof

An assembly method and supramolecular technology, applied in the preparation method of peptides, chemical instruments and methods, non-effective components of polymer compounds, etc., can solve problems such as biosafety, and achieve the effect of solving toxicity problems and biosafety problems

Active Publication Date: 2021-08-20
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The controllable disassembly supramolecule provided by the present invention can be used for drug delivery in vivo, and can be stored for a long time at -20°C, which solves the biological safety problem of the polypeptide supramolecular assembly in the current drug delivery system

Method used

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  • Controllable assembly and disassembly supramolecule and preparation method and application thereof
  • Controllable assembly and disassembly supramolecule and preparation method and application thereof
  • Controllable assembly and disassembly supramolecule and preparation method and application thereof

Examples

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Embodiment 1

[0046] This embodiment provides a controllable disassembly supramolecule with a molecular weight of 1028.1 g / mol; the controllable disassembly supramolecule is a nanofiber with a diameter of 10±1 nm, and has a structure as shown in formula I:

[0047]

[0048] This embodiment also provides a polypeptide supramolecular precursor for preparing the above-mentioned controllable disassembly supramolecular, which has the structure shown in formula II:

[0049]

[0050] The present embodiment also provides a method for preparing the above-mentioned polypeptide supramolecular precursor, comprising the following steps:

[0051] 1) Synthesize the target polypeptide sequence by solid-phase synthesis;

[0052] 2) Dissolve Tz-NHS, polypeptide and N,N-diisopropylethylamine (DIPEA) in chloroform at a molar ratio of 1.6:2:1, and stir at 30°C for 24h;

[0053] 3) Purify by semi-preparative HPLC (water in phase A, acetonitrile in phase B, both mobile phases contain 1 / 1000 TFA), and the s...

experiment example 1

[0060] The following experiments were performed on the supramolecules provided in Example 1:

[0061] 1. Transmission electron microscope experiment: through the above Figure 4 Compound B and compound C were used to make TEM samples and observed through transmission electron microscopy, and a large number of nanofiber structures could be seen in the stable hydrogel, while the samples made with C could not see nanofiber structures under the electron microscope. ( Image 6 In, TEM images of sample B compound (left) and C compound (right).

[0062] 2. Fluorescence spectrometer test: Thioflavin T (THT) is a common fluorescent dye used to label the β-sheet structure of amyloid fibrils. The binding of THT to amyloid fibrils produces strong fluorescence but almost no fluorescence in aqueous solution. The dye is capable of excitation at 440nm, resulting in an emission maximum wavelength around 480nm. exist Figure 5 10μM THT was added to the sample of .B, the excitation waveleng...

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Abstract

The invention provides a controllable assembly and disassembly supramolecule and a preparation method and application thereof. The controllable assembly and disassembly supramolecule has a structure as shown in a formula I. The controllable assembly and disassembly supramolecule provided by the invention can be used for drug delivery of a living body, can be stored for a long time at-20 DEG C, and solves the biosafety problem of polypeptide supramolecular assemblies in a current drug delivery system.

Description

technical field [0001] The invention relates to the field of nanometer supramolecular materials, in particular to a controllable disassembly supramolecular and a preparation method and application thereof. Background technique [0002] A drug delivery system is a device that uses a carrier or active substance to release drugs in a targeted and quantitative manner, which can improve drug delivery efficiency, targeting, absorption rate and reduce drug use. It has been rapidly developed since it was proposed. Nanomaterials are considered to be a major class of materials used as drug delivery vehicles. The design and modification of nanomaterials make them suitable for different delivery environments and have been extensively studied. As more and more nanomaterials are used as delivery vehicles, the accompanying safety issues of nanomaterials have also attracted intense attention. A large number of studies have shown that nanomaterials are biologically toxic and may exist in li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/107C07K1/107A61K9/06A61K47/42A61K47/54A61P35/00B82Y5/00B82Y40/00
CPCC07K5/1016A61K47/42A61K47/545A61K9/06A61P35/00B82Y5/00B82Y40/00
Inventor 高远宋嘉蕾
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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