73 results about "Pollen specific" patented technology

The invention provides an application of application of plant alpha-diastase in pollen abortion, and belongs to the technical field of genetic engineering. Through introducing polynucleotide of the plant alpha-diastase, recombinant vector and recombinant vector are structured; a pollen specific promoter drives the plant alpha-diastase to degrade starch in advance during the pollen developmental phase; the pollen sprouting energy cannot be provided, and the pollen sprouting is contained, thus abortion is generated; the transgenetic seedling of transgene pollen abortion is obtained. The plant alpha-diastase can be used as the pollen abortion gene and can be originated from genes of rice endogenesis, millet, broomcorn, barley, wheat and other gramineous plants; the plant alpha-diastase is very good for transgenic plant of pollen abortion. The plant alpha-diastase gene has accurate expression level and can control the spreading of transgenosis; the plant alpha-diastase gene also can keep and breed a male sterility line while leave out the artificial castration step during the hybrid seed production process; the application prospect is wide.

The invention relates to genetic engineering and molecular biology, and particularly discloses a plant anther specific promoter PCHF15 and application thereof. The invention further provides an expression vector with the plant anther specific promoter PCHF15, a genetic expression box and a carrier with the genetic expression box. The invention also provides a primer pair for amplifying the plant anther specific promoter PCHF15. The plant anther specific promoter PCHF15 is an endogenous gene of rice, is beneficial to genetic engineering of the rice, and drives exogenous genes to accurately express specificity in pollen, so that a novel method for driving specificity expression of the exogenous genes in the pollen is provided.

The invention provides a pollen and anther tapetum specific efficient promoter, the nucleotide sequence of which is shown as the sequence table SEQ ID No.1. The promoter sequence is cloned from a maize genome by screening in a maize genome library and can propel a target gene to have specific expression in the anther tapetum and the pollen. The invention comprises a plant gene engineering method for creating male sterileness by using the promoter sequence, namely, the promoter downstream is joined with a heterologous gene or a homologous gene, and a plant expression vector is constructed, a host plant is transformed, the promoter sequence can propel the downstream genes thereof to express the target protein in the anther tapetum and the pollen in a efficient and specific way to realize creating plant male sterileness or modifying the safety of transgenic plants.

The invention discloses a pollen-specific promoter and an expression vector thereof. Experiments show that the promoter can drive efficient and specific expression of a target gene in the pollen of transgenic rice. The promoter of the invention can be used for functional analysis and identification of genes related to growth and development of plant pollen, as well as creation of plant male sterile lines, prevention of plant transgenic drift, and the like. Especially, the promoter has an important application value in creation of male sterile lines of rice and other gramineous crops and utilization of heterozygous advantages.

The invention provides a pollen-specific promoter and application thereof. The promoter is derived from Brassica rapa and is an upstream sequence of a Delta8 sphingolipid desaturase gene. The promoter provided by the invention is a stable pollen-specific promoter having high specificity. The promoter can be used to promote the expression of various genes in pollen, and can be used for cultivatingmale sterile crop varieties and cultivating landscaping plants and the like with pollen eliminated or greatly reduced.

The invention provides cotton fiber and pollen specific expression promoter and an application. By the utilization of a genetic engineering technology, FPP1 promoters of GhFBP7 genes for cotton fiber and pollen specific expressions are successively separated. The FPP1 prompter contains 1283bp segment. The invention also verifies that the FPP1 promoter sequence has cotton pollen and fiber expression specificity. The promoter can guide specific expressions of cotton fiber and pollen in reporter gene and provides possibility and effective approaches to genetic engineering of raising cotton yield and quality.

A method for protecting a plant from high temperature stress (HTS), comprising transforming the plant with a polynucleotide sequence. The polynucleotide sequence comprises a nucleotide sequence encoding a sugar kinase under the control of an anther and / or pollen specific promoter, thereby producing a transformed plant having improved tolerance to HTS. Also disclosed is the polynucleotide sequence of the LeFRK4 promoter.

The invention separates and identifies a novel pollen-specific expression gene PSP231, wherein cDNA thereof comprises an open reading frame of 1656bp, and novel protein which contains 551 amino acid and unknown functions is encoded. The PSP231 gene is only expressed in a cotton male germ cell but not expressed in a nutritive cell. GUS activity analysis also proves that a PSP231 promoter has pollen-specific expression activity. The gene promoter contains a cytokinin KT response factor ARR1 binding site at the 236th site before an initiation codon; and the gene expression is significantly increased after KT processing, which shows PSP231 may be controlled by ARR1, and plays a certain role in a cell division process. The inhibition of the gene expression through an RNAi interference technology may cause pollen abortion. Contrarily, the over expression of the PSP231 gene in yeast can promote cell division and significantly increase the cell division index. The data shows that the PSP231 may receive mitosis treatment in cotton small spores, and play a very important role in the formation process of mature pollen.

A method for protecting a plant from high temperature stress (HTS), comprising transforming the plant with a polynucleotide sequence. The polynucleotide sequence comprises a nucleotide sequence encoding a sugar kinase under the control of an anther and / or pollen specific promoter, thereby producing a transformed plant having improved tolerance to HTS. Also disclosed is the polynucleotide sequence of the LeFRK4 promoter.

The invention relates to Sorghum bicolor plants which, due to modifications to the genome relating to a pollen-specific expression of patatin phospholipase, are capable of inducing haploidy, thereby producing haploid progeny, and within short time inbred lines, i.e. homozygous paternal parent and maternal parent lines can be produced by way of chromosome duplication for hybrid breeding. The invention also relates to methods for producing transgenic and non-transgenic plant haploid inducers and for improving the induction power of plants.

The application discloses a GAMYB gene based cultivation method for a transgenic rice sterile line. The GAMYB gene based cultivation method comprises the steps as follows: A, acquisition of a rice gene cassette; B, acquisition of a maize lethal gene ZMAA1; C, amplification of a maize pollen specific promoter Pg47; D, acquisition of an EGFP gene cassette; and E, connection of each gene expression element. The step E of connection of each gene expression element comprises: a first step of introducing a complete rice GAMYB gene into a pCAMBIA1300 vector, and then connecting the maize lethal geneZMAA1, the maize pollen specific promoter pPg47 and the EGFP gene expression element onto the pCAMBIA1300 vector. According to the invention, a F1-generation heterozygote follows the Mendel segregation law in the self fruitfulness process, and generated progenies not only contain heterozygotes which can keep a triple linked gene, but also contain the sterile line without fertility.

The invention provides a rice pollen specific expression promoter OsPoll2 and application thereof. The invention further provides an expression box and a recombinant expression vector containing the promoter; besides, the invention further provides a method for driving special purpose gene expression through the rice pollen specific expression promoter OsPoll2 in pollen of plants. The promoter is applied to crop genetic engineering. The promoter can specifically drive the target gene to be expressed in crop pollen, so that activity of the pollen can effectively changed, and the risk of transgenosis drifting caused by diffusion of active pollen is eliminated. The promoter is utilized in cooperation with the target gene with a pollen activity inhibition function to improve an existing transgenosis rice variety, excellent characters on other aspects of transgenosis rice are retained, the risk of gene drifting is also avoided, a good foundation is laid for deep research and application and popularization of the transgenosis variety, and effective safe guarantee is provided.

Provided is a seed sorting method for fixing plant heterosis. The invention provides a method for controlling and screening marker genes by a pollen specific genetic switching system and then sortingand cloning seeds so as to realizing the application of a hybrid seed non-fusion system. Three closely linked gene expression cassettes and a MiMe knockout vector are simultaneously transferred into ahybrid plant. The three gene expression cassettes include: 1) an embryonic autonomous gene expression cassette E1 driven by an oocyte specific expression promoter; 2) a screening marker gene expression cassette E2 regulated by E3; and 3) an expression cassette E3 for pollen-specific control of E2. In selfing fruiting seeds of hybrid transgenic plants, besides asexual embryo seeds which are fixedwith heterosis and are generated through parthenogenesis, zygotic embryo seeds formed by pollination and fertilization are also included; the two kinds of seeds are sorted by screening markers controlled by a pollen-specific gene switch, cloned seeds retain heterosis and can be used for production, and the remaining seeds can be used for commercial purposes.

The invention discloses plant pollen-specific promoter PSP1 and application thereof and also discloses an expression kit containing the plant pollen-specific promoter PSP1, a recombinant expression vector and a host cell, as well as a primer pair for amplifying the plant pollen-specific promoter PSP1, and a method of using the plant pollen-specific promoter PSP1 to drive target gene expression in plant pollen. A transgenic rice plant is acquired herein by transforming via the expression vector constructed with the plant pollen-specific promoter PSP1; verification and histochemical staining analysis show that the promoter can drive efficient specific expression of a target gene in pollen, and cloning of the plant pollen-specific promoter PSP1 helps develop seed breeding.

The invention provides millet alpha-amylase as well as an encoding gene and application thereof, belonging to the technical field of gene engineering. An amino acid sequence of the millet alpha-amylase is shown by SEQ ID No.4 or is an amino acid sequence which is obtained by substituting, deleting or adding one or more amino acids in the sequence and has the same function, and a gene encoded nucleotide sequence of the millet alpha-amylase is shown by SEQ ID No.1 or a sequence with 80% of homology with this sequence. Under the driving of a pollen specific promoter, the millet alpha-amylase provided by the invention degrades starch in advance in a pollen developmental phase, germination energy for pollen cannot be provided, and the germination of the pollen is restrained, resulting in male abortion of crops. The millet alpha-amylase provided by the invention can be used for keeping the homozygosis recessive state of a male sterile plant, and meanwhile, a step of artificial stamen removal during hybrid seed production is saved, so that the millet alpha-amylase has broad application prospect in the aspects of crop germplasm resource improvement and genetic breeding.

The invention discloses a cultivation method of a transgenic rice sterile line based on a ZMAA1 gene. The cultivation method includes the following steps: A, obtainment of a rice AID1 gene expressioncassette; B, obtainment of an mVenus gene expression cassette; C, amplification of a pollen specific promoter Pg47; D, amplification of a corn pollen lethal gene ZMAA1; and E, linkage of all gene expression elements. The step E which is the linage of all the gene expression elements further includes the following steps: firstly, linking the complete rice AID1 gene to a pCAMBIA1301 vector; and secondly, introducing the mVenus gene expression element to the pCAMBIA1301 vector which is introduced with the AID1 gene in the first step. An F1 generation heterozygote follows the Mendelian separationlaw in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

The application discloses an MSP1 gene based cultivation method for a transgenic rice sterile line, which comprises the steps as follows: A, acquisition of an mPlum gene cassette; B, acquisition of arice MSP1 gene cassette; C, amplification of a wheat lethal gene K1; D, amplification of a wheat pollen specific promoter Pg47; and E, connection of each gene expression element. The step E of connection of each gene expression element further comprises: a first step of connecting the mPlum gene expression element to a pCAMBIA1301 vector; and a second step of introducing a complete rice MSP1 geneinto the pCAMBIA1301 vector in which the mPlum gene expression element is introduced in the first step. According to the invention, a F1-generation heterozygote follows the Mendel segregation law in the self fruitfulness process, and generated progenies not only contain heterozygotes which can keep a triple linked gene, but also contain the sterile line without fertility.

The invention provides a method for inducing plant apomixis so as to fix heterosis. The method comprises the steps of shifting 4 closely-linked gene expression boxes into a hybridization plant, wherein the 4 closely-linked gene expression boxes comprise A, a lethal gene expression box E1 driven by egg cell or embryo specific expression promoters, B, an embryogenesis gene expression box E2 driven by specific expression promoters of somatic cells of integumentum and the like; C, a pollen inactivation gene expression box E3 driven by pollen specific expression promoters; and D, a screening markergene expression box E4; enabling integumentum somatic cells of heterozygosis transgenic plants in the selfing fructification process to form embryos, enabling sperms which do not carry transgenes tobe combined with central cells to form endosperms, and then performing development to form seeds; enabling the egg cells which do not carry transgenes to be normally fertilized with the sperms to fructify; and enabling the egg cells which carry the transgenes to be abortion and not participate in fertilization. Through the adoption of the method, the heterosis of hybridized seeds can be fixed, andseed production propagation is not needed.

The invention relates to a pollen specificity expression promoter and a plant expression vector thereof, wherein the promoter has a sequence under 1 item in a sequence list, with the length of 521bp, and a combined CaMV35S promoter replaces the plant expression vector pBI121 to construct a new plant expression vector which is named as pBITG6. The acquisition of the provided promoter creates conditions for a plant male sterile line and a restoring line obtained by using gene engineering, provides evidence for gene engineering research and provides basis for the specificity expression of an exogenous gene in the pollen. The exogenous gene can be a gene capable of coding disinsection toxin (targeting insects feeding on the pollen), a gene capable of increasing or decorating nutrient values of the pollen, and the like; and an excessive expression myrosase gene possibly has prevention function, and certain release function possibly on allergische rhinitis though applying prevention of pollen generation interfered by RNAi to roadside trees, thereby having important theoretical and practical meaning.

The invention discloses a method for overcoming incompatibility of distant hybridization stigmas of plants. According to the method, a pollination area of the stigmas is irradiated by using laser, the intensity and irradiation time of the laser are controlled, the pollination area of the stigmas is not destroyed completely, the identification of the stigmas on pollen specificity is reduced, so that the fertilization of ovules of paeonia lactiflora is completed, the problem of affinity between peony pollen and paeonia lactiflora stigmas is effectively solved, the fertilization process is normally completed, fusion of different genetic resources is effectively promoted, the genetic diversity of germplasm resources is further enriched, and a foundation is laid for further breeding of excellent new peony varieties. Meanwhile, the method can also be explored and applied to distant hybridization work of other plants with stigma incompatibility.

Sorghum plants are provided which are capable of inducing haploidy by modifications in the genome related to a pollen-specific expressed patatin phospholipid producing haploid offspring and can be produced for hybrid breeding in short time by chromosome doubling inbred lines, that is, homozygous father and mother lines. In addition, methods are provided for producing transgenic and non-transgenic plant haploid inducers and improving the induction performance of plants.

The application discloses a CYP703A3 gene based cultivation method for a transgenic rice sterile line, which comprises the steps as follows: A, acquisition of a rice gene cassette CYP703A3; B, acquisition of an sfGFP gene cassette; C, amplification of a wheat pollen lethal gene Ki; D, amplification of a wheat pollen specific promoter Pg47; and E, connection of each gene expression element. The step E of connection of each gene expression element comprises: firstly, introducing a complete rice CYP703A3 gene into a pCAMBIA1300 vector; and then connecting the sfGFP gene cassette, the wheat pollenlethal gene Ki and the wheat pollen specific promoter pPg47 to the vector. According to the invention, a F1-generation heterozygote follows the Mendel segregation law in the self fruitfulness process, and generated progenies not only contain heterozygotes which can keep a triple linked gene, but also contain the sterile line without fertility.

The invention discloses a pollen specific promoter sequence separated from corns and application of the pollen specific promoter sequence. The pollen specific promoter sequence is obtained by screening corn genome libraries and carrying out cloning in corn genomes. A pollen specific promoter disclosed by the invention comprises a nucleotide sequence represented by SEQ ID NO:1 or a specific fragment of the nucleotide sequence. The invention discloses a genetic engineering method for creating male sterile plants by virtue of the promoter sequence. More particularly, the method comprises the steps of linking a heterogenous or homologous gene to the downstream side of the promoter, constructing a plant expression vector, and transforming a host plant. By utilizing the promoter sequence, downstream genes can be driven to specifically express target proteins in pollen, so as to realize the creation of male sterile plants or the transformation safety of transgenic plants.

The invention belongs to the field of plant biotechnology, and relates to the separation and application of plant tissue specific promoters, in particular to application of a rape tissue specific promoter for regulating target genes expressed in plant anther. The promoter and the truncated fragments of the promoter are integrated with the reported genes and transformed into arabidopsis thaliana, and can drive the reported genes to express specifically in anther by GUS staining, and the sequences are shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4; after further truncation, a promoter fragment with a sequence of pollen specific expression shown as SEQ ID NO.5 is obtained. The invention further discloses that the tissue specific promoter or the truncated promoter fragments provide new effective regulatory elements for controlling plant fertility, and have a good application prospect for genetic engineering breeding.

The invention belongs to the field of plant gene engineering and plant breeding technology, concretely relates to a method, which comprises the following steps: dividing Barnase into an N terminal and C terminal, respectively using two pollen-specific promoters with overlapped expression periods to drive expression thereof in plant, thereby enhancing tissue specificity of Barnase expression, and overcoming toxicity leakage problem during establishment of plant male sterility line by using Barnase gene.

The invention discloses a corn pollen specific promoter and application thereof. The corn pollen specific promoter has a sequence shown as SEQ ID NO. 1 or a DNA sequence which can be hybridized with the sequence shown as SEQ ID NO. 1 under a strict condition and has promoter activity. The corn pollen specific high-efficiency expression promoter provided by the invention has important application value when a pollen abortion material is created by a genetic engineering means, for example, the promoter sequence is connected with an antisense gene of a gene necessary for pollen development or isconstructed into an RNA interference vector, and the gene necessary for pollen development can be silently expressed in pollen by transforming the RNA interference vector into a plant to generate a pollen abortion plant, so that adverse effects caused by continuous expression of a target gene at other parts are avoided. The invention provides a novel method for driving the target gene to be efficiently and specifically expressed in the pollen by using the promoter, and the method has wide application prospect in the aspects of genetic improvement of plants and research of plant bioreactors.