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Method for overcoming incompatibility of distant hybridization stigmas of plants

A technology of distant hybridization and stigma, which is applied in the field of plant distant hybridization, can solve problems such as inability to fertilize ovules and fail to complete normal development, achieve the effect of enriching genetic diversity and promoting the fusion of different genetic resources

Active Publication Date: 2021-08-20
LUOYANG ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the ovules are aborted in the middle and late stages of development, but some normal developments prove that laser irradiation can overcome the incompatibility of the stigma, which leads to the failure of fertilization and normal development of the ovules of the female parent peony.

Method used

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  • Method for overcoming incompatibility of distant hybridization stigmas of plants
  • Method for overcoming incompatibility of distant hybridization stigmas of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Select peony and herbaceous peony with strong growth potential, luxuriant branches and leaves, and strong branches as test materials respectively, select herba peony B63 as the female parent and Paeonia delavayi as the male parent to carry out hybridization, and use the method of overcoming plant distant hybridization of the present invention. A stigma-incompatible method comprising the steps of:

[0027] Step 1, detasseling; the day before the female parent of Paeoniae Alba B63 is about to open, peel off the flower buds manually, and pull out the stamens with tweezers, taking care to pull them out completely; at the same time, pay attention to shielding when detasseling, complete the work quickly, and put on a breeding bag isolation;

[0028] Step 2, laser irradiation on the stigma; at ten o'clock in the morning on the second day after emasculation, open the breeding bag for observation. If there is obvious mucus on the stigma, laser irradiation can be performed on the...

Embodiment 2

[0034] Select peony and peony with strong growth potential, luxuriant branches and leaves, and strong branches as test materials respectively, select peony B63 as the female parent and peony "Crown" as the male parent to carry out hybridization, and use the method of the present invention to overcome the stigma instability of distant hybridization of plants. Affinity method, including the following steps:

[0035] Step 1, detasseling; the day before the female parent of Paeoniae Alba B63 is about to open, peel off the flower buds manually, and pull out the stamens with tweezers, taking care to pull them out completely; at the same time, pay attention to shielding when detasseling, complete the work quickly, and put on a breeding bag isolation;

[0036]Step 2, laser irradiation on the stigma; at ten o'clock in the morning on the second day after emasculation, open the breeding bag for observation. If there is obvious mucus on the stigma, laser irradiation can be performed on th...

Embodiment 3

[0042] Select peony and peony with strong growth potential, luxuriant branches and leaves, and strong branches as test materials respectively, select peony B63 as the female parent and peony "Golden Pavilion" as the male parent to carry out hybridization, and use the stigma of overcoming plant distant hybridization of the present invention A non-affinity method, including the following steps:

[0043] Step 1, detasseling; the day before the female parent of Paeoniae Alba B63 is about to open, peel off the flower buds manually, and pull out the stamens with tweezers, taking care to pull them out completely; at the same time, pay attention to shielding when detasseling, complete the work quickly, and put on a breeding bag isolation;

[0044] Step 2, laser irradiation on the stigma; at ten o'clock in the morning on the second day after emasculation, open the breeding bag for observation. If there is obvious mucus on the stigma, laser irradiation can be performed on the stigma. Ea...

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Abstract

The invention discloses a method for overcoming incompatibility of distant hybridization stigmas of plants. According to the method, a pollination area of the stigmas is irradiated by using laser, the intensity and irradiation time of the laser are controlled, the pollination area of the stigmas is not destroyed completely, the identification of the stigmas on pollen specificity is reduced, so that the fertilization of ovules of paeonia lactiflora is completed, the problem of affinity between peony pollen and paeonia lactiflora stigmas is effectively solved, the fertilization process is normally completed, fusion of different genetic resources is effectively promoted, the genetic diversity of germplasm resources is further enriched, and a foundation is laid for further breeding of excellent new peony varieties. Meanwhile, the method can also be explored and applied to distant hybridization work of other plants with stigma incompatibility.

Description

technical field [0001] The invention relates to the technical field of plant distant hybridization, in particular to a method for overcoming plant distant hybridization stigma incompatibility. Background technique [0002] In the process of plant breeding, the use of distant hybridization between species and genera with relatively distant kinship can produce more abundant genetic variation than conventional sexual hybridization between species within a species, which is not only one of the effective ways for germplasm innovation , and has important theoretical significance in the study of biological issues such as the formation, evolution and phylogeny of species. However, in the long-term evolution process of plants, various isolation mechanisms have been formed, and distant hybridization is very difficult. The incompatibility of distant hybridization and the sterility of hybrids are the main reasons for the slow progress of plant distant hybridization breeding. At the sam...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02Y02P60/14
Inventor 马会萍彭正锋李军胡晓亮杨海静冀含乐王晓辉卢林韩鲲丁建兰
Owner LUOYANG ACADEMY OF AGRI & FORESTRY SCI
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