Rice pollen specific expression promoter OsPoll2 and application thereof

A pollen-specific, promoter technology, applied in the fields of biotechnology and crop genetic engineering, can solve the problems of high plant growth and development, the time or space of target gene expression cannot be well controlled, and the improvement effect is not obvious.

Active Publication Date: 2016-06-22
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0005] At present, some constitutive strong promoters are widely used in the field of agricultural biotechnology, such as the CaMV35S promoter and the corn Ubiquitin-1 promoter. However, when using these promoters to induce the target gene to transform rice and other crops to impro...

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  • Rice pollen specific expression promoter OsPoll2 and application thereof
  • Rice pollen specific expression promoter OsPoll2 and application thereof
  • Rice pollen specific expression promoter OsPoll2 and application thereof

Examples

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Embodiment 2

[0059] In this example, the present invention uses the above-mentioned rice pollen-specific expression promoter OsPoll2 to drive the antisense fragment of the OsRIP gene (LOC_Os12g03822).

[0060] First, using a similar method as above, the rice pollen-specific expression promoter OsPoll2 is first ligated upstream of the antisense fragment of the target gene to obtain a crop expression vector in which the promoter OsPoll2 and the antisense fragment of the target gene are fused.

[0061] Next, the combination product of rice pollen-specific expression promoter OsPoll2 and the antisense fragment of the target gene was transferred into Agrobacterium tumefaciens. The Agrobacterium tumefaciens transformed with the conjugate product is used to transform the seeds of the target plant. Finally, the transformed seeds were used to cultivate the corresponding plants. In the obtained plants, it was found by potassium iodide staining that part of the pollen was aborted and the pollen activity w...

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Abstract

The invention provides a rice pollen specific expression promoter OsPoll2 and application thereof. The invention further provides an expression box and a recombinant expression vector containing the promoter; besides, the invention further provides a method for driving special purpose gene expression through the rice pollen specific expression promoter OsPoll2 in pollen of plants. The promoter is applied to crop genetic engineering. The promoter can specifically drive the target gene to be expressed in crop pollen, so that activity of the pollen can effectively changed, and the risk of transgenosis drifting caused by diffusion of active pollen is eliminated. The promoter is utilized in cooperation with the target gene with a pollen activity inhibition function to improve an existing transgenosis rice variety, excellent characters on other aspects of transgenosis rice are retained, the risk of gene drifting is also avoided, a good foundation is laid for deep research and application and popularization of the transgenosis variety, and effective safe guarantee is provided.

Description

Technical field [0001] The invention relates to the technical fields of biotechnology and crop genetic engineering. Specifically, the present invention relates to a rice pollen-specific expression promoter and its application. The promoter can drive the specific expression of target genes in pollen in crop genetic engineering, thereby effectively preventing and controlling transgene drift by changing pollen vigor. Background technique [0002] Transgenic technology is considered to be one of the most effective ways for humans to solve the contradiction between the sharp decline of land resources and the rapid expansion of population. However, due to the short development and utilization of this technology, humans have very limited understanding of its biological safety. And among the public, many people have concerns about the safety of plant genetically modified technology. Exogenous genes carried by transgenic plants may drift with pollen to closely related species or wild spe...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/11C12N15/82C12N15/84A01H5/00
CPCC07K14/415C12N15/8205C12N15/8231
Inventor 魏鹏程杨剑波李浩李娟李莉杨亚春秦瑞英许蓉芳
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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