A rice pollen-specific expression promoter ospoll2 and its application

Abstract

The invention provides a rice pollen specific expression promoter OsPoll2 and application thereof. The invention also provides an expression box and a recombinant expression vector containing the promoter. In addition, the present invention also provides a method for using the rice pollen-specific expression promoter OsPoll2 to drive the expression of a specific target gene in plant pollen. And the present invention applies the above-mentioned promoter to crop genetic engineering. The promoter provided by the present invention can specifically drive the expression of the target gene in the crop pollen, thereby effectively changing the vigor of the pollen and eliminating the risk of transgene drift caused by the spread of vigorous pollen. Using the promoter of the present invention to cooperate with the target gene with the function of inhibiting pollen viability to improve existing transgenic rice varieties can not only retain the excellent traits of transgenic rice in other aspects, but also avoid the risk of gene drift, which is an in-depth study of transgenic varieties It has laid a good foundation for promotion and application, and provided effective security protection.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and crop genetic engineering. Specifically, the present invention relates to a rice pollen-specific expression promoter and its application. The promoter can drive the specific expression of target genes in pollen in crop genetic engineering, thereby effectively preventing and controlling transgene drift by changing pollen vigor. Background technique [0002] Transgenic technology is considered to be one of the most effective ways for human beings to solve the contradiction between the sharp decline of land resources and the rapid expansion of population. However, due to the short period of development and utilization of this technology, human beings have very limited understanding of its biological safety. Many people in China and the general public have concerns about the safety of plant transgenic technology. The exogenous genes carried by transgenic plants may drift to related species ...

AI Technical Summary

Problems solved by technology

[0005] At present, some constitutive strong promoters are widely used in the field of agricultural biotechnology, such as the CaMV35S promoter and the corn Ubiquitin-1 promoter. However, when using these promoters to induce the target gene to transform rice and other crops to improve crop quality , the improvement effect is often not obvious because the time or space of the expression of the target gene cannot be well controlled, or the growth and development of the plant are affected because the gene expression induced by these constitutive promoters is too high

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