127 results about "Corn pollen" patented technology

The invention discloses a rice genetic engineering sterile line breeding method. The method includes following steps: A, acquiring a DsRED gene expression box; B, acquiring a rice EAT1 gene expressionbox; C, amplifying corn lethal gene ZMAA1; D, amplifying corn pollen specific promoter Pg47; E, connecting gene expression elements: step 1, connecting a DsRED gene expression element onto a pCAMBIA1300 carrier; step 2, introducing a complete rice EAT1 gene onto the pCAMBIA1300 carrier where the DsRED gene expression element is already introduced in the step 1. F1-generation heterozygote abides by Mendel's law of segregation in the process of self fruiting, and generated offspring include heterozygote capable of maintaining triply linked genes and infertile sterile lines.

The present invention discloses a qi benefiting and blood nourishing fragrant and hot walnut kernel which is made of following raw materials in parts by weight: walnut kernels 80-90 parts, watermelon seed kernels 10-20 parts, fish roes 20-30 parts, corn pollen 7-8 parts, oolong tea 8-10 parts, papaya 8-10 parts, capsicum frutescens 4-5 parts, kadsura longipedunculata 2-2.4 parts, elaeagnus pungens 0.7-1.5 parts, Chinese wolfberry fruits 0.7-2 parts, glycerol, sesbania gum, zein, and an appropriate amount of 3%-4% sodium carbonate aqueous solution. The qi benefiting and blood nourishing fragrant and hot walnut kernel utilizes zein to conduct film coating treatment on walnut kernels, which can completely cut off walnut kernels from the outside moisture and oxygen, inhibit oxidative and rancid process of walnut kernels, and extend the shelf life of walnut kernels; and the added kadsura longipedunculata, elaeagnus pungens and other traditional Chinese medicines increase the health-care efficacies of walnut kernels in clearing liver and improving eyesight, supplementing blood and beautifying features, and calming mind and benefiting qi.

The invention discloses resistance enhancement instant gruel, which is prepared from the following raw materials: rice, black rice, oat, corn, soybean, black soya bean, germinated brown rice, white hyacinth bean, kidney bean, asparagus, carrot, eggplant, lentinula edodes, Agrocybe aegerita, agaric, spirulina, corn pollen, echinacea, helichrysum ledifolia, balm, lemongrass, glutathione, glutathione, vitamin A, vitamin C, vitamin E, selenium-enriched malt, zinc protein, aloin, salt, soybean oligosaccharides and direct vat set. The resistance enhancement instant gruel is comprehensive in nutrition, moderate in thickness, soft, glutinous and nice in flavor and sweet and sauce in taste; the food materials are abundant and can provide nutrient substances for human, so that the resistance is obviously enhanced, and the resistance enhancement instant gruel has the functions of fighting bacterium and diminishing inflammation, fighting cancer, helping digestion, caring skin, delaying senescence and preventing cardiovascular and cerebrovascular diseases; the instant gruel does not have any additive, so as to be safer and healthier in eating; the instant gruel is convenient to carry and eat, so as to be in accordance with the fast pace of life.

The invention provides a hazelnut-pomegranate health-care rice cake. The rice cake is prepared from the following components by weight: 80 to 90 parts of glutinous rice, 20 to 30 parts of black rice, 10 to 12 parts of Chinese chestnut, 4 to 6 parts of hazelnut, 9 to 10 parts of pomegranate, 3 to 4 parts of muskmelon, 4 to 6 parts of Chinese cherry, 3 to 4 parts of fig, 10 to 11 parts of a pitaya flower, 8 to 9 parts of mint, 4 to 6 parts of Chinese wolfberry bud, 6 to 8 parts of Xueyingzi, 2 to 4 parts of a prepared rehmannia root, 3 to 5 parts of a sunflower head, 2 to 4 parts of a Chinese hawthorn seed, 4 to 6 parts of glutinous rice root hair, 1 to 2 parts of tree peony bark, 8 to 10 parts of Chinese yam, 3 to 4 parts of testiset penis phocae, 2 to 3 parts of licorice, 3 to 4 parts of oroxylum indicum vent and 0.1 to 0.3 part of corn pollen. The health-care rice cake provided by the invention has delicate taste, fragrant smell and the effects of enriching yin, nourishing kidney, strengthening stomach and promoting digestion and is applicable to a variety of populations.

The invention discloses aralia elata seem kidney-tonifying biscuits made from the following raw materials in part by weight: 30-40 parts of glutinous rice flour, 10-15 parts of kudzuvine root powder, 10-15 part of chestnut powder, 3-4 parts of purple potato starch, 8-9 parts of lobster shells, 15-17 parts of vegetable oil, 3-4 parts of phospholipid, 0.2-0.3 part of corn pollen, 5-6 parts of maltose, 2-4 parts of dry skim milk, 1.2-1.5 parts of ammonium bicarbonate, 0.1-0.3 part of calcium gluconate, 0.05-0.08 part of zinc gluconate, 0.5-0.6 part of low sodium salt, 0.1-0.2 part of flavouring agent, 1-2 parts of salvia miltiorrhiza, 10-12 parts of aralia elata seem, 3-4 parts of grape leaves, 1-2 parts of hawthorn seed powder, 3-5 parts of rehmannia leaves, 0.2-0.3 part of fenugreek, 0.4-0.6 part of walnut meat and an appropriate amount of water. The aralia elata seem kidney-tonifying biscuits have the characteristics of low fat, low sugar and high calcium and meanwhile contain very high dietary fiber, and long-term consumption of the aralia elata seem kidney-tonifying biscuits can achieve the functions tonifying the kidney and strengthening the spleen.

The invention discloses a corn seed production pollinating machine. The corn seed production pollinating machine comprises a pollinating gun body, wherein a handle is fixedly arranged at one end of the lower side of the pollinating gun body; an air pressing cavity is arranged in one end of the pollinating gun body; a rubber piston is arranged in the air pressing cavity; a piston rod is mounted at one end of the middle of the rubber piston; the piston rod penetrates through an air pressing hole and a push-pull handle is fixedly mounted at one end of the piston rod; the pollinating gun head is arranged at one end of the pollinating gun body; a pollinating pipe is connected with one end of the air pressing cavity; the pollinating pipe penetrates through one end of the pollinating gun head; the upper side of the pollinating gun body is in thread connection with a dust collection funnel; the upper end of the dust collection funnel is connected with a funnel cover through a hinge; a powder discharging valve is arranged at the lower side of the dust collection funnel; the lower end of the dust collection funnel is communicated with the pollinating pipe; the lower side of the air pressing cavity is connected with an air pump pipe. According to the corn seed production pollinating machine, corn pollen is sprayed through air pressure; the corn seed production pollinating machine has a relatively good pollinating effect, and is simple to operate and convenient to use, and the efficiency of artificial pollination is improved.

The invention discloses a feed additive for reducing cow body cells and treating recessive mastitis and a preparation method of the feed additive. The feed additive is prepared from the following raw materials in parts by weight: 10-15 parts of antibacterial peptide, 5-10 parts of geobacillus sp, 10-15 parts of yeast, 15-20 parts of cottonrose hibiscus leaf, 20-30 parts of andrographis paniculata, 10-20 parts of thesium chinense, 5-10 parts of cowherb seed, 15-20 parts of mongolian dandelion herb, 10-20 parts of epimedium, 20-30 parts of astragalus membranaceus, 5-10 parts of lindera aggregata leaf, 1-5 parts of sea buckthorn seed powder, 1-5 parts of corn pollen, 0.2-0.3 part of organic chelated zinc, 0.3-1 part of organic chromium and 8-15 parts of coated threonine. The additive can quickly reduce the body cell number, quickly cure the recessive mastitis, enable a cow to quickly restore to be the healthy production state, avoid generation of clinical mastitis, comprehensively improve the immunologic function of the body of the cow in the lactation period, effectively prevent generation of various diseases, highly improve the anti-stress capability of the cow in the perinatal period, effectively prevent generation of antenatal and puerperal diseases, highly improve the vaccine protection rate, strengthen the immune antibody level, and avoid immune clearance, immune inhibition and vaccine stress.

The invention discloses a DNA introduction method independent of a corn genotype. Magnetic nanoparticles with positive charges are adopted as a gene carrier to be combined with DNA with negative charges, under the action of a magnetic field, the magnetic nanoparticles combined with the DNA transfer the DNA into corn pollen through pollen holes, and introduction of a target gene is realized throughnatural reproduction processes of pollination and fruiting. According to the invention, efficient introduction and transient and stable expression of the target gene in all corn can be realized, andthe bottleneck problems that current mainstream corn gene introduction methods based on tissue culture are limited by a few acceptor materials and have low introduction efficiency and complicated operation are solved.

The invention discloses soft-shelled turtle feed capable of enhancing a health function on human bodies by soft-shelled turtle meat and a preparation method of the soft-shelled turtle feed. The soft-shelled turtle feed is prepared from the following raw materials in parts by weight: 250-300 parts of fish scales, 200-250 parts of shrimp powder, 100-150 parts of peanut cakes, 70-80 parts of soybean cakes, 10-20 parts of bean curd dregs, 10-20 parts of apple dregs, 40-50 parts of ryegrass, 30-40 parts of forage maize, 2-3 parts of white atractylodes rhizomes, 2-3 parts of hawthorn fruits, 1-2 parts of elecampane, 2-3 parts of chicken's gizzard-membrane, 50-60 parts of garlic peels, 150-200 parts of dried blood, 50-80 parts of chicken powder, 70-80 parts of corn flour, 40-60 parts of barley flour, 10-20 parts of soybean oil, 4-6 parts of soybean sauce, 30-50 parts of goat milk, 10-20 parts of pumpkin seed powder, 5-8 parts of corn pollen, 4-5 parts of red date powder, 3-5 parts of grape seed extract, 2-3 parts of medical stone powder and 0.5-1 part of vanillin. The feed disclosed by the invention has comprehensive nutrients, is delicious and palatable, is convenient to use and has remarkable benefits.

The invention discloses an immunity-improving colla corii asini and jujube capsule which is prepared from the following raw materials in parts by weight: 25-30 parts of colla corii asini powder, 25-30 parts of jujube extract powder, 1-2 parts of black sesame powder, 3-5 parts of corn pollen, 0.5-1.0 part of grape seed extract powder and 20-25 parts of Chinese herbal medicine extract powder, wherein the Chinese herbal medicine extract powder is prepared from the following raw materials: ginseng, sea-buckthorn, yams, sealwort, the fruit of Chinese wolfberry, wild jujube seeds, lilies, lotus seeds, peach kernels, hawthorn, fructus cannabis, fingered citron, rose flowers and liquorice. An animal function test proves that the colla corii asini and jujube capsule has a health-care function of improving immunity.

The invention discloses a laying hen feed additive. The laying hen feed additive comprises the following materials: 0.3-0.8 part of choline chloride, 4-8 parts of earthworm powder, 0.3-1.0 part of duckweed, 3-5 parts of feather powder, 2-3 parts of yeast powder, 5-10 parts of pine needle powder, 0.05-0.1 part of ciprofloxacin, 3-5 parts of corn pollen and 0.1-0.2 part of vitamin C. The laying hen feed additive can be prepared by adopting a traditional process. The laying hen feed additive has the following characteristics: laying hens are promoted to take food by the intercoordination of the components in the additive, the stress capability and immunity of the laying hens are enhanced, and the egg yield is significantly improved after the feed additive is taken by the laying hens. The laying hen feed additive is pollution-free and healthy.

The invention discloses a preparation method of a Chinese patent health granule containing a green tea extract. The method comprises the following steps: (1) ethanol extraction to obtain an ethanol extract concentrated solution; (2) water extraction to obtain a water extract concentrated solution; and (3) powder preparation, namely mixing the ethanol extract concentrated solution with the water extract concentrated solution, drying in vacuum to obtain a dry extract, crushing the dry extract, screening by a mesh sieve to obtain extract powder, adding the green extract powder, corn pollen, pueraria powder and a seasoner, mixing evenly, preparing soft materials, pelletizing, drying, arranging granules, and packaging, so as to obtain the Chinese patent health granule containing the green tea extract. The granule is convenient to carry and convenient to use, the use of a green tea product is expanded; meanwhile, the granule has prevention and health effects on high blood pressure, high blood fat and high blood glucose; food is produced according to a pharmaceutical production standard; the product quality is ensured; and meanwhile, the nutritional ingredients of the components can be effectively extracted.

The invention discloses a wheat ear in-vitro culture method through wheat and corn hybridization and haploidembryo induction. The method comprises the steps of clipping the wheat ear after pollination by corn pollen for 24h from the field or the pot, spraying 100mg / L 2,4-D solution on the wheat ear, inserting into a container containing ear culture solution, placing the container in a manual climatic box for continuous culture through day and night, replacing the ear culture solution for the first time after three days, replacing the ear culture solution for the second time after three days, then replacing the ear culture solution for the third time after the next four days until the culture is completed, wherein in the 13th-14th days from the culture starting to the culture ending, the ear culture solution is replaced to submerge 2-3cm of the stem of the wheat ear each time, and the stem and the leaves of the wheat ear need to be treated when the ear culture solution is replaced each time, at least 2-3cm of the base stem of the wheat ear is cut into a plain end by scissors, and then the leaves which do not grow on the nodes of the base part of the stem are removed. With the adoption of the method, the haploidembryo inductivity can reach more than 70% and the embryos grow well.

The invention discloses a corn cross pollination method. The method comprises the following steps: detasseling a corn female parent, and cutting 2-7cm of top ends of female spikes before silks reel off from the female spikes; correspondingly bagging the female spikes of the female parent and the male spikes of a male parent; cutting the male spikes of the male parent of the corn 12-24h before pollen dispersing of the male spikes of the male parent of the corn, collecting pollen from the male spikes after pollen dispersing, drying and freezing the pollen; unfreezing the frozen pollen before pollination, and then performing the first and second hand corn cross pollination based on different silk reeling off degrees of the male parent of the corn. With the adoption of the pollination method,the problem that the flowering time is not met due to the weather can be prevented; the pollination demand of the female parent of the corn can be met; a pollen increasing agent and pure corn pollen are mixed and sprayed for pollination, so that the quantity of used pure pollen is decreased, and as a result, the quantity of planted cross corn male parents can be decreased; the corn product obtained with the pollination method is high in maturing rate; the corn seeds are high in hundred-grain weight; the stem empty rate is low; and the improvements on economic benefits of corn planting are facilitated.

The invention provides a whole-nutrition formula food suitable for obesity and defatting surgery patients in convalescence. Selenium-enriched plant protein powder is used as a main nutrient component, various Chinese herbal medicines frequently used for reducing blood lipid, reducing cholesterol and reducing weight are added, based on the selenium-enriched plant protein powder and the added Chinese herbal medicines, radish seeds, poria cocos, corn pollen and citrus medica are added, wherein the radish seeds have the effects of promoting digestion, eliminating swelling, depressing Qi and resolving phlegm and contribute to adjusting the functions of the intestine and the stomach of the postoperative patients, the poria cocos not only has the effect of adjusting the functions of the intestine and the stomach but also has the functions of invigorating the spleen and tranquilizing mind, the corn pollen can adjust the microbial population of the intestine and the stomach, and the citrus medica is mainly used for treating distending pains of the stomach and the abdomen, indigestion, reversed flow of qi and vomiting and also has the effect of adjusting the functions of the intestine and the stomach of the postoperative patients; the raw materials have synergistic reaction, and are much better in effects when being used together than effects when being used singly; and oligomerization mannose is added, the absorption of intestinal tracts is facilitated, and effects on middle-aged people and old people with bad absorption functions of the intestinal tract are notable. The whole-nutrition formula food disclosed by the invention is made into powder by a conventional method to be eaten for a long term, and absorption is easy.

The invention provides an artificial supplementary pollination tool for corns, which relates to the technical field of corn breeding and is used for solving the problems that pollens carry anthers or other impurities so that the pollens are impure and further the efficiency is low in an existing corn pollen collection and pollination manner. The artificial supplementary pollination tool comprises a bottle bottom, a first bottle body, a second bottle body and a third bottle body which are detachably connected in sequence; a bottle opening is formed in a free end of the third bottle body and a bottle cap is arranged on the bottle opening; a second mesh screen is arranged at one end, which is connected with the second bottle body, of the first bottle body, and is provided with a second through hole through which only the pure corn pollens can pass; and a first mesh screen is arranged in the bottle opening and a first through hole through which the pollens with the average diameters of 40-50 microns can pass is formed in the first mesh screen. The tool can be used for screening the collected pollens to obtain the pure corn pollens. Meanwhile, the bottle bodies are pressed to spray out the pollens and the operation is convenient and simple; and the bottle bodies can be immediately recovered after being released from the hand, so that the supplementary pollination can be carried out again and the pollination efficiency is high.

The invention discloses a pig feed for increasing appetite and preventing diseases and a preparation method thereof. The pig feed is prepared from the following raw materials in parts by weight: 30-50 parts of wheat, 20-30 parts of sorghum, 10-15 parts of wheat middling, 5-10 parts of chrysanthemum meal, 10-15 parts of cereal chaff, 4-8 parts of bovine colostrum powder, 6-12 parts of biscuit dregs, 3-5 parts of maize pollen, 2-4 parts of eucommia ulmoides leaves, 3.5-4.5 parts of oyster sauce, 3-4 parts of neroli oil, 2-3 parts of bamboo vinegar, 4-6 parts of chanterelle powder, 1-3 parts of gynostemma pentaphylla, 1.5-2.5 parts of cashew kernels, 2-3 parts of torreya grandismerrillii, 2-3 parts of pearl powder, and 3-5 parts of modified mountain flour. The pig feed is not only good in palatability, and can increase the appetite of pigs and improve the feed intake of pigs, but also can provide nutritional ingredients such as rich proteins, minerals, vitamins and the like, and also can enhance the immunity of pigs, enhance the disease resistance of pigs, and lower the incidence of diseases, thereby increasing the growth speed of pigs, reducing the cultivation cost, and improving the slaughtering rate of pigs.

The invention discloses an enzymolysis method of pollen. The method comprises the following steps: S1, enzymolysis: taking 5Kg of pine pollen, 10Kg of corn pollen and 5Kg of rape pollen, performing wall breaking, adding the obtained pollen into a 100L reaction kettle, adding 80Kg of purified water, 100g of cellulase and 100g of alpha-cellulose into the reaction kettle, heating the mixture in the kettle to 40+ / -5 DEG C, performing reaction for 5+ / -0.5 hours, heating the obtained material to 80 DEG C, then adding 100g of protease, and performing reaction for 12 hours; S2, filtration: performingfiltration while the obtained mixture is hot after the reaction is finished, and taking filtrate; and S3, concentration: performing concentration under reduced pressure until the density is about 1.1,and discharging extractum. The method is simple and safe in process, and does not use other organic reagent compared with traditional enzymolysis water extraction. Besides, the enzymolysis of the invention is full, and meanwhile, the content of micromolecular peptides after enzymolysis is 30% or above. The method greatly improves the absorption effect for the human body, and is suitable for popularization and use.

The invention discloses a misgurnus anguillicaudatus red-fin disease prevention and treatment feed additive, which is prepared from the following raw materials: agrimonia pilosa ldb, artemisia anomala s. moore, fructus viticis, nidus vespae, periostracum cicadae, Xiangmo, faeces trogopterpri, myrrh, frankincense, semen persicae, lancea tibetica, adhatodavasicanees, halenia corniculata, adder tongue herb, ginkgo leaf, prunusmlzmesieb.etzucc, pine pollen, corn pollen, compound enzyme A, and compound enzyme B. According to the present invention, the misgurnus anguillicaudatus red-fin disease prevention and treatment feed additive has the rich aroma, and further has effects of misgurnus anguillicaudatus food intake increasing, growth and development promoting and resistance enhancing, wherein the incidence of the red-fin disease is reduced to 3-4%, the mortality rate is reduced to 0%, the disease treatment rate is 84%, and the income is increased by 17.8%; the pollens are added, such that the nutrition and the fragrance are enriched, the intestine and the stomach of the misgurnus anguillicaudatus are protected, and the wound healing is accelerated; and a variety of the traditional Chinese medicine extracts are added, such that the advantages of scientific compatibility, complement efficacy, antibacterial effect, anti-inflammation effect, no drug resistance generation, misgurnus anguillicaudatus immunity enhancing, no agent residue, delicious misgurnus anguillicaudatus meat taste, fishy smell reducing and health function increasing are provided, and the modern consumer demand is met.

The invention discloses a corn pollen health beverage. The corn pollen health beverage is prepared from the following raw materials: corn pollens, sweet potatoes, Chinese yams, crataegus pinnatifida bunge, mulberry juice, abelmoschus esculentus juice, celery juice, sesames, pumpkins, chitosan oligosaccharide, sodium alginate, sodium carboxymethylcellulose and water. The health food has good effects of promoting digestion, repairing stomach and intestine damages and treating astriction. Meanwhile, the health beverage further has the sweetness and fragrance of the corn pollens, the sweet potatoes, the mulberry juice and the pumpkins, has the sour taste of crataegus pinnatifida bunge and is sour and sweet, and tasty.

The invention relates to a ginseng-astragalus pollen tablet and a manufacture method thereof. The ginseng-astragalus pollen tablet provided by the invention comprises the following components in portion by weight: 4 to 200 portions of codonopsis pilosula, 4 to 200 portions of hoantchy roots, 1 to 50 portions of honey, 1 to 50 portions of cattail pollen, 1 to 50 portions of rape pollen, 1 to 50 portions of corn pollen and 1 to 50 portions of sunflower pollen. The manufacture method for the ginseng-astragalus pollen tablet provided by the invention comprises the following steps that: an equivalent amount of codonopsis pilosula and hoantchy roots are weighed; effective ingredients are extracted from the codonopsis pilosula and the hoantchy roots, concentrated, dried and made into dry extract; the dry extract is ground and made into medicinal powder; the cattail pollen, the honey, the rape pollen, the corn pollen and the sunflower pollen are weighed, well mixed, fermented, dried, ground and well mixed with the medicinal powder; mixed powder is added with subsidiary substances, stirred, well mixed and made into a preparation. The ginseng-astragalus pollen tablet has the advantages of improving the tolerance of organisms under an oxygen-deficient condition, delaying senility and strengthening the pharmaceutical nourishing-invigorating effects of the codonopsis pilosula and the hoantchy roots.

The invention discloses soft-shelled turtle feed improving fattening efficiency and a preparation method thereof. The feed is prepared from, by weight, 400-500 parts of meat and bone meal, 150-200 parts of fish meal, 100-150 parts of soybean protein powder, 70-90 parts of corn flour, 10-20 parts of yeast protein, 40-50 parts of wheat bran, 20-30 parts of distillers' grains, 10-20 parts of sweet potato residues, 10-20 part of sugarcane residues, 30-40 parts of lotus leaves, 30-40 parts of alfalfa, 20-30 parts of carrots, 2-3 parts of cyrtomium fortunei, 2-3 parts of rhizoma atractylodis, 2-3 parts of fennel, 1-2 parts of cinnamon, 1-2 parts of liquorice roots, 50-60 parts of apple seeds, 5-8 parts of corn pollen, 4-6 parts of grape seed extracts, 1-1.5 parts of vanilla powder, 1-2 parts of wood vinegar, 50-70 parts of sea fish oil and 0.3-0.5 part of salt. The soft-shelled turtle feed is rich in animal protein, plant protein, starch, grease, crude fibers, vitamin and mineral substance, comprehensive in feed nutrition and delicious.

The invention discloses a corn pollen collecting device. The corn pollen collecting device comprises a pollen collector, wherein the pollen collector is funnel-shaped; a connecting part is arranged atthe lower end of the pollen collector; the connecting part is connected with a container through threads; a handheld rod connecting part is arranged on an outer wall of the upper end of the pollen collector; a handheld rod is in threaded connection with the interior of the handheld rod connecting part; a V-shaped opening is formed in a side wall of the pollen collector; a rectangular hole is formed in the lower end of the V-shaped opening; a power device is arranged in the rectangular hole; a micro vibration motor and a battery are arranged in the power device; a power switch is arranged on the outer surface of the power device; and the power switch is electrically connected with the battery and the motor. During operation, the length of the handheld rod can be adjusted to be matched withthe V-shaped opening, excessive bending and breaking of tassels are effectively avoided, pollen collection is facilitated, and meanwhile, the labor intensity is reduced. All corn tassels are placed in the collector, so that pollen drifting is avoided. By using the vibration motor, the pollen collection time is effectively shortened, and the collection efficiency is improved.

The invention discloses an improved corn pollen magnetic transfection method. The method comprises the following steps: (1) preparing plasmid DNA for transfection; (2) combining nanomagnetic beads MNP with the plasmid DNA under a room temperature condition to form an MNP-DNA compound; (3) collecting fresh pollen of corn in a full-bloom stage, and quickly taking the pollen back to a room under a water-proof ice box preservation condition; (4) uniformly mixing corn pollen preservation liquid with sieved corn pollen, and performing trepanning pretreatment under a low-temperature condition; (5) adding the MNP-DNA compound into a trepanning pretreatment solution, conducting gentle and uniform mixing, and carrying out transfecting on a low-temperature pre-cooled magnetic plate; and (6) after the transfection is finished, carrying a pollen suspension to a field by using an ice box, and directly pollinating corn ears. According to the method, the corn planted in the field is directly utilized for transformation, only conventional refrigeration equipment is needed, operation is easy and convenient, and cost is low, so the method is suitable for all corn varieties.

The invention relates to the field of plant biotechnology and genetic breeding technology, in particular to an application of plant amino acid permease and coding genes thereof to regulation and control of high temperature resistance of plants. The invention discloses participation of a ZmAAPa gene of corn amino acid permease in regulation and control of the tolerance of corn pollen to high temperature stress. The knockout of the ZmAAPa gene can significantly improve the tolerance of the corn pollen to the high temperature stress. A corn material with the ZmAAPa gene knocked out is obtained through a CRISPR / Cas9 technology and corn genetic transformation, the germination rate of the corn pollen under the high temperature stress condition is remarkably increased through knockout of the ZmAAPa gene, and normal development of pollen and anther is not affected.

The invention discloses an application of a Zmcps gene to preparation of a male sterile line of corn, and belongs to the technical field of gene engineering. The invention provides the application ofthe Zmcps gene to preparation of the male sterile line of the corn. A protein Zmcps influencing male fertility can effectively realize complete abortion of corn pollen so as to be used for male sterile seed production of the corn, the corn seed production purity is improved, and the seed production cost is reduced.