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Corn cross pollination method

A hybrid pollination and corn technology, applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, can solve the problem of less application of corn, and achieve the effects of improving seed production efficiency, reducing planting costs, and increasing yield

Inactive Publication Date: 2019-02-26
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign research on medium pollen is mainly concentrated in fruit trees, tobacco and vegetables, but there are few related applications in corn.

Method used

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  • Corn cross pollination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The corn hybrid pollination method of the present invention comprises the steps:

[0032] (1) Take emasculation treatment to the female parent corn plant before the tassels loose powder, then cut off 2 cm from the top of the ear before the ear is drawn, put a sulfuric acid paper bag on the ear and fix the sulfuric acid paper bag on the ear with a pin; When the ear filaments of the bag corn female parent exposed the bract 2cm, the tassel of the corn male parent was covered with a sulfuric acid paper bag, and the sulfuric acid paper bag was fastened tightly with a paperclip at the tassel base;

[0033] (2) 24 hours before the tassel pollination of the male parent of corn, the bagging on the tassel is picked off and the tassel is cut off, the pollen on the tassel is collected and the pollen is dried and frozen, and the collected pollen is collected at 25 ℃ in an incubator until the water content of the pollen is 4%, passed through a 100-mesh sieve, added glycerin at a soli...

Embodiment 2

[0038] The corn hybrid pollination method of the present invention comprises the steps:

[0039] (1) Take emasculation treatment to the female parent corn plant before the tassel pollination, then cut off 3 cm from the top of the ear before the ear is drawn, put a sulfuric acid paper bag on the ear and fix the sulfuric acid paper bag on the ear with a pin; When the ear filaments of the bag corn female parent exposed the bracts by 2.5 cm, the tassel of the corn male parent was covered with a parchment paper bag, and the parchment paper bag was fastened tightly with a paper clip at the tassel base;

[0040] (2) 12 hours before the tassel pollination of the male parent of corn, the bagging on the tassel was picked off and the tassel was cut off, the pollen on the tassel was collected and the pollen was dried and frozen, and the collected pollen was collected at 26 ℃ in an incubator until the water content of the pollen is 8%, pass through a 120-mesh sieve, add ethylene glycol at ...

Embodiment 3

[0045] The corn hybrid pollination method of the present invention comprises the steps:

[0046] (1) Detasseling the female corn plants before the tassels loose powder, then cutting off 4 cm from the top of the ear before silking the ear, putting on a parchment bag and fixing the parchment bag on the ear with a pin; When the female ear filaments of the bag corn female parent exposed the bracts by 3 cm, put the tassel of the male parent of corn on a parchment paper bag, and fasten the parchment paper bag tightly with a paperclip at the tassel base;

[0047] (2) 20 hours before the tassel pollination of the male parent of corn, the bagging on the tassel was picked off and the tassel was cut off, the pollen on the tassel was collected and the pollen was dried and frozen, and the collected pollen was collected at 27 ℃ in an incubator until the water content of the pollen is 15%, passed through a 180-mesh sieve, added propylene glycol at a solid-to-liquid ratio of 1.8:1 and mixed e...

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Abstract

The invention discloses a corn cross pollination method. The method comprises the following steps: detasseling a corn female parent, and cutting 2-7cm of top ends of female spikes before silks reel off from the female spikes; correspondingly bagging the female spikes of the female parent and the male spikes of a male parent; cutting the male spikes of the male parent of the corn 12-24h before pollen dispersing of the male spikes of the male parent of the corn, collecting pollen from the male spikes after pollen dispersing, drying and freezing the pollen; unfreezing the frozen pollen before pollination, and then performing the first and second hand corn cross pollination based on different silk reeling off degrees of the male parent of the corn. With the adoption of the pollination method,the problem that the flowering time is not met due to the weather can be prevented; the pollination demand of the female parent of the corn can be met; a pollen increasing agent and pure corn pollen are mixed and sprayed for pollination, so that the quantity of used pure pollen is decreased, and as a result, the quantity of planted cross corn male parents can be decreased; the corn product obtained with the pollination method is high in maturing rate; the corn seeds are high in hundred-grain weight; the stem empty rate is low; and the improvements on economic benefits of corn planting are facilitated.

Description

technical field [0001] The invention relates to the technical field of corn planting, in particular to a method for cross pollination of corn. Background technique [0002] Maize is an important cereal widely grown in tropical and temperate regions of the world. At present, my country's sown corn area is about 300 million mu, second only to rice and wheat, and ranks third among food crops, second only to the United States in the world. [0003] Maize is a cross-pollinated plant. The pollen of the male flower falls with the wind to the filaments of the ear to fertilize and solidify, completing the alternation of generations. In addition, maize is a typical outcrossing crop, showing extreme inbreeding depression and heterosis, so maize is one of the most successful crops for heterosis utilization, and can increase yield by more than 20%. Hybrid seed production by artificial emasculation is the main way to utilize heterosis in maize. In the process of hybrid seed production,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 石达金吕巨智范继征唐国荣程伟东杜桂衡钟昌松闫飞燕
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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