211results about How to "Improve seed production" patented technology

Methods for increasing the product yield from plants, preferably transgenic plants, are provided. It has been discovered that in vitro cultures from donor plants produce plants that have two or three fold increase in product yield. One embodiment provides a method for increasing product yield from a transgenic plant by initiating an in vitro culture from a donor transgenic plant, wherein the donor transgenic plant is genetically engineered to produce a product and regenerating a second transgenic plant from the in vitro culture, wherein the yield of the product from the second transgenic plant is greater than the yield of the product from the donor transgenic plant. In a preferred embodiment, the transgenic plant is a graminaceous plant such as switchgrass.

The invention discloses a seed reproduction and high-yield cultivation technical specification of onion, which aims at providing the seed reproduction and high-yield cultivation technical specification of the onion against the problem that the prior seed reproduction of the onion is lack of the quipped cultivation technical measures, so that the seed production is low and unstable. The seed reproduction according to the specification has the advantages of simple and scientific cultivation method, high seed production, labor-saving property, low cost, stable seed nature and high purity.

The invention relates to a breeding method of a high-quality disease-resistant high-temperature-resistant rice two-line sterile line, and belongs to the technical field of crop genetic breeding. The method is involved in rice germplasm breeding with long grain polymerization, high head rice rate and excellent economical characters such as high seed production temperature resistance and disease resistance, and the rice two-line sterile line with long grains, high head rice rate, high seed production temperature resistance, good out-crossing feature, stable fertility, good disease resistance and excellent comprehensive characters are bred. Great assembling is carried out by utilizing the sterile line, and a novel two-line hybrid rice assembly which is high in quality, disease-resistant and beneficial for seed production is screened out hopefully.

The invention provides a humic acid water-soluble fertilizer which comprises the following components (expressed in mass percent): 0.8 to 8.4% of potassium fulvicate, 5.6 to 21.4% of diammonium phosphate, 0.12 to 0.54% of ammonium molybdate, 0.125 to 0.575% of copper sulfate, 0.1 to 0.4% of ferrous sulfate, 0.8 to 3.6% of boric acid, 3.8 to 12.5% of potassium dihydrogen phosphate, 0.75 to 1.75% of zinc sulfate, 0.25 to 1.15% of manganese sulfate and 57.5 to 75.5% of water. According to the invention, fulvic acid and essential nutrients for plants are used as components of the fertilizer, plant nutrients are in a reasonable ratio, and the humic acid water-soluble fertilizer is characteristic of capacity of significantly promoting early maturity of and preventing premature senility of grain crops and economic crops like fruits and vegetables.

The invention relates to a method for cultivating cross rice. Wherein, it comprises that (1), selecting the light-temperature sensitive sterile system with low-temperature sterile or short-light low-temperature sterile; (2), selecting the sterile masculinity as gene system; (3), crossing sterile system S with sterile masculinity system S', to obtain stable sterile masculinity S / S' F1; (4), screening and recovering system R; (5), using S / S' F1 as mother type to be crossed with recover system R, to find the needed cross combination, to produce cross rice. The invention can improve the match property of sterile system and reduce produce cost.

Novel gibberellin 2-oxidase (GA2ox) genes were identified. Differential expression of GA2ox genes correlated with flower development, seed germination, tiller growth and other developmental processes. In addition, the early and increased growth of tiller and adventitious root and altered root architecture caused by overexpression of GA2oxs further suggest the pleiotropic role of GA2oxs in controlling growth and architecture in plants such as rice. GA2ox5, GA2ox6 and GA2ox9 were three genes encoding class C20 GA2oxs in rice. Mutants or transgenic rice overexpressing class C20 GA2oxs exhibited a broad range of mutant phenotypes, including semi-dwarfism, increased root system and higher tiller numbers that may favor grain yield. Mutations in the conserved domain III were found to affect the physiological activity of class C20 GA2oxs. Methods are described for controlling plant growth and architecture by controlling gene expression of gibberellin 2-oxidase in the plant.

The invention relates to a hybrid round eggplant seed production method and belongs to the technical field of vegetable seed breeding. The method comprises the steps of sowing, planting, pruning, pollinating and harvesting and is characterized in that: during the pruning, four-branch pruning is performed; door eggplants and opposite eggplants are removed; and pollination and fruits reservation are performed after buds of bucket-shaped eggplants are matured. The method has the advantages of simpleness, easy fruit setting, unified nodal region, nutrition insurance, uniform fruits, low seed production cost, high plant stress resistance, low fruit morbidity, many seeds in a single plant, high seed production yield and the like.

The invention discloses a breeding method of an insect-resistant big-stigma three-line sterile line, belonging to the technical field of rice insect-resistant breeding. The breeding method is characterized in that a three-line maintainer line B is taken as a recurrent parent, a chilo-suppressalis-resisting parent, a rice-planthopper-resisting parent and a big-stigma parent are subjected to hybridization, backcross and insect-resistant screening to polymerize big stigma, chilo-suppressalis-resisting and rice-planthopper-resisting genes in the three-line maintainer line B, and then the highly chilo-suppressalis-resisting and planthopper-resisting big-stigma three-line sterile line is formed through hybridization and backcross of the line B and a corresponding line A. The sterile line is highly resistant to rice planthopper and chilo suppressalis, is easy to expose because of large stigma of glumous flowers, and is high in exposure rate of the stigma, outcrossing maturing rate and seed production yield; and the seed production yield and the insect-resistant level of hybrid rice can be improved when the insect-resistant big-stigma three-line sterile line is used for preparing the hybrid rice.

A method for culturing the seeds of photosensitive and thermo-sensitive male sterility line of high-temp sterile or long-day high-temp sterile rice includes such steps as choosing male parent and female parent (photosensitive and thermo-sensitive sterility line), sowing, irrigating with cold water when the ambient temp is higher than the critical temp of fertility transform, hybridizing, growing and harvesting.

The production of biomass and / or seeds is further increased. The production of biomass and / or seeds by a plant can be further increased by supplying glutathione to a plant into which a gene encoding protein phosphatase 2C having characteristic consensus sequences has been introduced.

The invention discloses a hybrid seed production method for forage maize. In the method, teosinte serves as a male parent and maize tri-crossed hybrid serves as a female parent to produce a forage maize SC2 seed in a hybrid way. The hybrid production comprises the following steps of: 1) sowing the male parents and the female parents at time intervals to obtain the male parents having blossom intervals and the female parents having the blossom intervals; 2) performing hormone spraying and tassel removal on the female parents having the blossom intervals obtained in the step 1) to obtain the tassel-removed female parents having lower plant heights and lower ear heights; 3) trimming the subtending leaves of the tassel-removed female parents having the lower plant heights and the lower ear heights obtained in step 2) to obtain un-pollinated female parents; and 4) collecting the pollen of the male parents having the blossom intervals obtained in the step 1), pollinating the un-pollinated female parents obtained in the step 3) by using the pollen and culturing the pollinated female parents to obtain the forage corn SC2 seeds. Experiments of the invention show that plant obtained by planting the forage maize SC2 seed obtained by the method of the invention is gramineous pasture having high quality.

The invention relates to a fast creating method for a rice long-grain japonica type indica-compatible two-line sterile line germplasm and belongs to the technical field of crop genetic breeding. The method relates to creation of the long-grain japonica type indica-compatible rice germplasm. By taking the two-line sterile line as a sterile gene donor, the rice two-line sterile line is bred by combining techniques of anther culture, natural condition and manual climatic box fertility identification and the like, wherein the plant shape is japonica type, the grain is the long-grain japonica, the flowering time is early, the fertility is stable and the comprehensive character is excellent. The sterile line is used for massive assembly of hybrid rice new combination, so that a first generation of strong heterotic indica / japonica hybrid is expected to be screened, thereby laying a foundation for effectively utilizing advantages of rice indica / japonica hybrid.

A hybrid pansy seed production method is carried out by the steps of seeding, seedling growing, field planting, field management, pollination, picking, post-picking processing and the like. Compared with the prior art, the hybrid pansy seed production method has the advantages of high seed yield, good seed quality, virus infection resistance and the like.

A breeding method for a sterile line of the japonica rice with a high stigma exsertion rate relates to:(1) selecting the materials with big stigmas or high stigma exsertion rate; (2) hybridizing a donor parent carrying the genes of the stigma exsertion characters with a parent material of the japonica rice; (3) selecting the single plant with a stigma exsertion rate more than 95 percent to acquire a maintenance line with a stigma exsertion rate more than 95 percent beginning from the F2 generation of a hybrid segregative generation;(4) using the maintenance line obtained from step (3) to hybridize with the sterile line of the japonica rice carrying sterile genes and using the maintenance line as a recurrent parent to carry out continuous backcross to obtain a sterile line of the japonica rice with a high stigma exsertion rate and basically stable characters. The invention has the advantages of that: 1. the invention is suitable for maintaining the existing sterile line, carrying out alteration on the stigma exsertion rate and improving the stigma exsertion rate of the existing sterile line of the japonica rice; 2. the invention can directionally realize the alteration on the improvement of the stigma exsertion rate on all materials including the maintenance line of the existing sterile line of the japonica rice with a lower stigma exsertion rate, thus further improving the yield of seed production.

The invention discloses a paddy rice female sterility gene and an application thereof in hybridized paddy rice seed production. The 8th site nucleotide residue of the second exon of the paddy rice Os03g0215200 gene is mutated from T to C, such that a paddy rice mutant of female completely sterile phenotype with cernuous leaves is obtained, and is named sfs1. The female sterility is caused by that the exostome is blocked by outer integument abnormal proliferation of cells, and that fertilization can not be achieved. The paddy rice mutant is utilized as a male parent, and is hybridized with an existing restoring line. Rotational crossing and high-generation homozygous are carried out, such that a novel female completely sterile restoring line is obtained by screening. Mixed-sowing seed production is carried out based on the restoring line and a male sterile line female parent, such that seed production and seed reproduction yields can be improved, seed production cost can be reduced, purity of the hybridized seeds can be ensured, and mechanized production and light cultivation of the hybridized paddy rice seeds can be completely realized.

The present invention discloses a perennial forage maize breeding method. Experiments prove that the character of high seed rate from hybridizing diploid maize and tetraploid Zea perennis is a heritable dominant character. On the basis, the present invention provides a method for breeding Zea mays L. with high seed rate which is hybridized with Zea perennis, the F1 generation of Zea mays L. obtained by the method is used as a female parent, tetraploid Zea perennis is used as a male parent for artificial hybridization, and the obtained hybrid seeds of F1 generation are perennial forage maize seeds. The perennial forage maize bred by the method has the characteristics of short breeding cycle, easiness in operation and high seed yield, and has broad application prospects in production.

The invention belong to the field of using the advantage of hybrid rice. It leads the long ear-neck gene eui of the masked long-stalked rice '02428h' into the biliner sterile strain by backcross, and gets the technology of breeding long-stalked biliner sterile strain. The ear-neck length is similar to that of sterile strain not containing eui gene with 450-600 grams of '920' per hectare, the iso-cross property of sterile strain is basically improved, the productivity of seed is increased and the production cost is reduced.

The invention discloses a breeding method for improving yield and seed production purity of hot pickled mustard tubers. According to the method, big-plant reserved seeds, medium-plant reserved seeds and small-plant reserved seeds of the hot pickled mustard tubers are respectively seeded in different periods; planting of plants, fertilization, seed selection, pruning and harvesting are performed on big, medium and small hot pickled mustard tuber plants by adopting different treatment means; and the seeds of the hot pickled mustard tubers are produced by adopting a repeated reproduction technical route of reproducing big-plant reserved seeds with the big-plant reserved seeds, reproducing medium-plant reserved seeds with the medium-plant reserved seeds and reproducing production seeds with the small-plant reserved seeds. The method has the advantages of greatly improving the yield and the purity of the hot pickled mustard tuber seeds and reducing the cost and the risk of seed production.

The invention relates to a method for breeding a sterile round-shaped rice with exposed high stigma, comprising the following steps: (1) crossing round-shaped rice with Shan rice and getting F1; (2) backcrossing the F1 with round-shaped rice to get BC1F1; (3) getting BC1F2 through BC1F1 with high exposed stigma rate auto-crossing and getting BC2F1 through backcrossing with round-shaped rice; (4) planting the BC1F1 in large group for two generations, auto-crossing and collecting together, getting BC3F1; (6) planting BC2F2 in large group, autocrossing and collecting together, getting BC2F3; (7) BC3F1 autocrossing, collecting together and getting BC3F2; (8) planting BC1F4, BC2F3 and BC3F2 in large group respectively, chossing strains and breeding sterile strain; (9) getting stable sterile strain through successive backcross with sterile strain for 4-5 generations. The invention can effectively increase the exposed stigma rate of round-shaped rice from 10-20% to above 60%, and thus increase the fruit-producing rate of the hybrid and increase the productivity.

A method for mechanized seed production of hybrid rice through integration of the seedling slinging technology and the direct seeding technology particularly includes the steps that the number of days of difference from sowing to heading of male parents and female parents is calculated, seedlings of parents (normally the male parents) with a long growth period are grown in advance (preparation of seedling slinging is made), and when the seedling age approaches the number of the calendar period difference days from sowing to heading, mechanized seedling slinging or manual seedling slinging is conducted in advance on a prepared field according to a certain proportion so that seedlings can be evenly distributed in the field; meanwhile, seeds of parents (normally female parents) with a short growth period are soaked at the right time, germination is accelerated, according to the difference between calendar periods from sowing to heading, the seeds are evenly and directly sowed in the field (in a mechanized mode), so that the condition that the male parents and the female parents are planted in the field at the same period is guaranteed, the parents are evenly mixed, the mode that the parents are planted in different rows in traditional seed production is broken, labor is saved, field management is facilitated, mechanized harvest is achieved with the combination of the mixed sowing seed production technology (including the male parent herbicide sensitivity method, the glume color selection method and the like), and therefore mechanization of the whole process of seed production is achieved.

The invention discloses a method for breeding and producing of hybrid seeds for hybrid rice II super 42. The breeding method comprises the following steps: A. taking Zhenhui129 as female parent and Zhenhui084 as male parent to get seed F0; B. auto-copulating for 5-7 generations and choosing at the same time plants with strong restoration and disease-resistant ability, short leaf, compact plant type and good appearance, cultivating out restoration system Zhenhui42 of new plant type, disease-resistant and high productivity; C. crossing sterile system II-32A with said restoration system Zhenhui42 and getting new rice breed II super 42 with high leaf blight-resistant property, short leaf and compact plant type. The rice got in this invention is characterized by the good comprehensive productivity, moderate maturity time; sound property; outstanding disease-resistant property and stable productivity.

The present invention provides methods and compositions for increasing the seed size and / or seed number in plants. In particular, the methods and compositions provide for the over expression of a plant growth and / or development related or associated gene during embryo development. Transgenic plants transformed with genetic constructs having the plant growth and / or development associated gene under the control of an early phase-specific embryo promoter provides mature plants in the field that produce larger and / or more seeds. Methods for selection growth and development associated genes that provide transgenic plants with a higher yield phenotype are also provided.

The invention provides a breeding method of entire silo corn hybrids. The breeding method comprises the steps of breeding a parent selfing line, isolating spatially, artificially hybridizing, bagging for isolation and selfing for achieving homozygosity; performing test mating on a selfing line A and a selfing line B to obtain a single cross hybrid F1; and finally, carrying out test mating on the single cross hybrid F1 used as a female parent and a third selfing line C used as a male parent to breed the entire silo corn hybrid (three-way cross hybrid). The breeding method adopts three selfing lines to breed the entire silo corn hybrid and opens up a new method for breeding of the silo corn hybrid; the bred three-way cross hybrid has the characteristics of high quality, high biological yield and high seed production, contains more than 10% of crude protein, lower than 45% of neutral detergent fibers and lower than 25% of acid detergent fibers; two selfing lines are subjected to test mating to obtain the single cross hybrid, then the single cross hybrid used as the female parent and another one selfing line used as the male parent are used for seed production, and therefore, the seed production yield is ensured and the seed production cost can be reduced.

The invention discloses a breeding method of an ornamental purple-yellow-leaf rape line. According to the method, a cabbage type rape radish cytoplasmic male sterile line with lack of green core leaves is used as a female parent, a purple-red leaf cabbage type rape line is used as a male parent, and hybridization is conducted to obtain a first-filial generation; and the first-filial generation isbackcrossed with the purple-red leaf cabbage type rape line so as to obtain rape of the ornamental purple-yellow-leaf rape line. The ornamental purple-yellow-leaf rape line is a novel ornamental oilseed rape having yellow core leaves and other purple-red leaves and obviously different from the leaf colors of other rapes. The rape can be used in tourist attractions, parks, urban squares, urban roadgreening and other tourist, sightseeing and leisure places; the rape has high ornamental value as people can enjoy the sight of the leaves of the rape in a seedling stage and the flowers of the rapein a flowering stage; and an ornamental period of the rape is six months or more.

The invention discloses a one-seeding one-stubble double-harvest bitter gourd seed production method. After seed collection is carried out for the first time, the plant is not directly pulled out, butrather a manner of pruning and reserving side tendrilled vine is adopted, lateral branches are promoted from the base of the main tendrilled vine by a great amount of water and fertilizer, when the first batch of the seed bitter gourd is harvested and the length of the side tendrilled vine is 50-100 cm, the main tendrilled vine is cut off at the position of 5-10 cm above the side tendrilled vine,the side tendrilled vine is led on the frame, and side tendrilled vine is used for continuous seed production. The seed production method has the advantages that the secondary seeding is omitted, theoriginal seed is saved, the time and labor are saved, and the seed production yield is increased.

The invention discloses a seed production method for reducing the rotting rate of broccoli, and belongs to the technical field of plant cultivation. The method includes the steps: seeding in a proper seeding period of seed production parents; seeding in a potted tray; spreading seeds; performing unified management after seedlings emerge; planting the seedlings on a land for growing field crops with the planting space of 45*40cm after each seedling grows to have four leaves and a center; and cutting off ball flowers and long main stems 15cm below the ball flowers together when the ball flowers of the broccoli grow to have the diameter ranging from 2cm to 3cm. During seeding in the potted tray, the potted tray is filled with matrixes, water is poured over the potted tray, holes are pressed, one seed is placed in each hole, the seeds are covered with the matrixes after seeding, and a small amount of water is poured. During spreading, a flat land with the area of about 10g / m<2> in a greenhouse is selected, special matrixes for raising seedlings are uniformly laid, the thickness of the special matrixes is about 3 centimeters, water is poured over the special matrixes, the seeds are uniformly spread, a layer of matrixes with the thickness of 0.3 centimeter is spread on the seeds again, and then a small amount of water is poured. By the method, the rotting rate of seed production plants of the broccoli can be greatly reduced, the seeds are fine, and the seed yield is improved by more than 50%.

The invention relates to a method for breeding a broad-spectrum rice restorer line hybrid rice combination, and belongs to the technical field of plant breeding. The method comprises the following steps: breeding strong superiority restorer line 'Yanhui 1393' with compact plant type, bacterial blight resistance-moderate resistance, rice blast resistance-moderate resistance, excellent quality and broad spectrum; carrying out measurement matching on the Yanhui 1393 with an indica three-line sterile line (Tianfeng A, Quan 9311A, Quanxiang 79A, Zhenxian 1A and the like) and an indica two-line sterile line (salt 220S, Y58S, depth 08S, Quan 03S and the like); successively breeding a new strong superiority series three-line hybrid indica combination (Tianfeng superior 1393, Quan superior 1393, Quanxiang superior 1393 and Zhenxian superior) with moderate mature stage, compact plant type, bacterial blight resistance-moderate resistance, rice blast resistance-moderate resistance, high and stable yield and excellent rice quality and a new strong superiority series two-line hybrid indica combination (salt two-superior 1393, Y two-superior 1393, depth two-superior 1393 and new two-superior 1393).

The invention discloses a seed production method of hybrid rice, which comprises the following steps of: (1) sowing of seeds and culture of seedlings: determining the sowing time according to the sowing periods of a male parent and a female parent to enable the flowering periods of the male parent and the female parent to be synchronized; (2) field management: carrying out water draining and field drying management based on shallow water mainly, covering the water in time after field drying to prevent and eliminate plant diseases and insect pests, and spraying '920' with power-operated spraying and dusting equipment to expel male parent pollen; (3) impurity prevention and preservation of pure state: ensuring that no sticky rice exists in the range of 200 meters beyond a seed production region and only the male parents of the seed production combination can be planted in the range of 20 meters beyond the seed production region, and killing off abnormal plants before flowering of cereal crops and loosing of powder to ensure the seed purity; and (4) harvesting and storing: cutting off the male parents in the seed farm to ensure that no male parent exists in the seed farm, and clearing a harvesting machine in the processes of harvesting and going to the field to ensure that no other seeds and impurities exist in the harvesting machine. The invention has the characteristics of high working efficiency, less labor demands, low cost, high purity, high quality, high yield and the like.

The invention provides a zucchini breed hybrid seed production method. In the zucchini breed hybrid seed production method, through all-around improvement in various stages, namely preparing a seed farm and soil, determining a ratio of male parents to female parents, the sowing time and the sowing method, manually pollinating, managing after pollination and harvesting, the occupied area of the male parents is effectively reduced, the number of the male parents is increased (in an ordinary breeding method, the ratio of the male parents to the female parents is 1: 4 and the male parents occupy a larger area), the growth period is prolonged, the aging of plants is slowed, and a seed production yield of a zucchini breed is improved, up to 70-80kg per about 1 / 6 acre; produced hybrid seeds are good in plumpness and shiny in seed skin and have the seed purity of over 99%. Therefore, the zucchini breed hybrid seed production method is suitable for large-scale popularization.

The invention provides a method for improving the bee colony pollination efficiency in a seed production shed. The life cycle of the preliminary bee specie of worker bees is selected as the number of early days, and a bee colony is organized, so that after a bee colony beehive is put in the shed and pass the life cycle, new eggs produced by a queen bee after being put in the shed grow to adult bees, and at the moment the plant to produce seeds blooms. In the life cycle of the new eggs growing to the adult bees, the bee feed formed by mixing the pollen and syrup of the plant to produce seeds is adopted for continuously feeding, so that the specific training of flower visiting for the seed producing plant for the bees can be realized, and the adaptability to shed environment of new worker bees and old bees is strong. After entering the flowering phase, feeding with the bee feed is stopped, and the new worker bees and the old bees can only actively collect honey from the flowers of the seed producing plant, so that the flower visiting frequency is greatly increased, the pollination efficiency and the seed production yield are greatly improved, and the loss of the trained bee colony is greatly reduced.