Novel cotton gene PSP231 and promoter thereof

Abstract

The invention separates and identifies a novel pollen-specific expression gene PSP231, wherein cDNA thereof comprises an open reading frame of 1656bp, and novel protein which contains 551 amino acid and unknown functions is encoded. The PSP231 gene is only expressed in a cotton male germ cell but not expressed in a nutritive cell. GUS activity analysis also proves that a PSP231 promoter has pollen-specific expression activity. The gene promoter contains a cytokinin KT response factor ARR1 binding site at the 236th site before an initiation codon; and the gene expression is significantly increased after KT processing, which shows PSP231 may be controlled by ARR1, and plays a certain role in a cell division process. The inhibition of the gene expression through an RNAi interference technology may cause pollen abortion. Contrarily, the over expression of the PSP231 gene in yeast can promote cell division and significantly increase the cell division index. The data shows that the PSP231 may receive mitosis treatment in cotton small spores, and play a very important role in the formation process of mature pollen.

Description

technical field [0001] The present invention relates to cotton genes. Specifically, it is the cloning and functional identification of a new pollen-specific expression gene PSP231 and its promoter. This gene is involved in the regulation of cell division and plays a very important role in the development of cotton pollen. Background technique [0002] For a long time, cotton has been an important economic crop in my country. Cotton production is not only directly related to the income of Chinese farmers, but also related to the healthy development of the textile industry and the employment of about 20 million textile workers. With the development of industry and the improvement of people's living standards, people's demand for high-quality textiles is gradually increasing. Therefore, while the demand for cotton in the textile and related industries increases, they also have higher requirements for the quality of cotton fiber. At present, the annual output of raw cotton in...

AI Technical Summary

Problems solved by technology

Because of the long flowering period of cotton, no effective chemical andricides have been found for field seed production. Therefore, the hybrids that are widely promoted in my country's production are still mainly artificial emasculation.

This method requires a lot of labor and the cost of seed production is high, which limits the utilization of cotton heterosis to a large extent.

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