Promoter with anther tissue specificity

Abstract

The invention discloses a promoter with anther tissue specificity. The promoter with the anther tissue specificity is as follows (a) or (b) or (c): (a), 3'end contains at least 1-1698 nucleotide sequences of a sequence 1 in a sequence table, beginning at the 1698 position of sequence 1 and extending to the 3' end of sequence 1 in accordance with the nucleotide sequence of the sequence 1, expandingto the 3' end of the sequence 1 according to the nucleotide sequence of the sequence 1, and any DNA fragment of length from 1698 to 2205bp is obtained; and a DNA molecule has a promoter function; (b)a DNA fragment is provided with a defined identity of 75% or more than 75% of the nucleotide sequence limited by (a), and provided with the promoter function; and (c) a DNA fragment hybridizes with the nucleotide sequence limited by (a) or (b) under strict conditions and is provided with the promoter function. The promoter can be used for the specific expression of exogenous genes in anthers andthe creation of male sterile and restorer lines, and a wide application prospect is achieved.

Description

technical field [0001] The invention relates to an anther tissue-specific promoter in the field of biotechnology. Background technique [0002] Plant gene regulation is mainly carried out at the transcriptional level, which is coordinated by a variety of cis-acting elements and trans-acting factors. The promoter is an important cis-acting element. It is a DNA sequence located in the upstream region of the 5' end of the structural gene to regulate gene transcription. It can activate RNA polymerase to accurately combine with the template DNA to ensure accurate and efficient transcription. play a key role in transcriptional regulation. According to the different characteristics of their driving gene expression, promoters are divided into constitutive promoters and specific promoters: constitutive promoters can initiate transcription in all cells or tissues regardless of time and space; specific promoters can also It is divided into tissue-specific promoters and inducible prom...

AI Technical Summary

Problems solved by technology

At present, some constitutive strong promoters are widely used in the field of agricultural biotechnology, such as the CaMV35S promoter of cauliflower mosaic virus and the Ubiquitin-1 promoter of maize. When it is expected to improve the quality of crops, the improvement effect is not obvious because the time (developmental stage-specific) or space (tissue-organ-specific) of the expression of the target gene is not well controlled, or because these constitutive promoters induce gene Too high expression level will cause metabolic burden and affect the growth and development of plants. These are the obstacles encountered in the current use of constitutive strong promoters combined with functional genes to improve crop quality

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