The invention discloses a method for genetic targeting of stem spermatogonium of poultry. By using an adenovirus of poultry as a targeting vector, the method performs targeting operation on the stem spermatogonium of the poultry; the obtained genetically modified stem spermatogonium can be used for transplanting testis and obtaining a transgenetic gene; and by mating the normal poultry, the stem spermatogonium can be further used for breeding the transgenetic poultry. The method has the advantages that: the method can effectively realize the high-efficiency genetic targeting of the stem spermatogonium of the poultry; the used homologous arm is short, and the vector is easily established; and after the exogenous objective albumen is directly integrated in an albumin expression albumen high-efficiency promotor, the expressional specificity of the exogenous albumen of the transgenetic offspring is high, the yield of albumen is high. Moreover, the method also has the advantages of simple operation, safety, no pathogenicity, low immunogenicity and less cell toxins.