85 results about "Spectinomycin" patented technology

The invention relates to a spectinomycin colloidal gold detection card. According to the method, a sample liquid absorbing part, a colloidal gold labeling part, a detection reaction part and a water absorbing part are orderly stuck on a backing of test paper. The detection reaction part is coated by an antigen 1 bar which is applied to detection and is used as a detection line, and at the same time is coated by an IgG1 bar which resists the second species of animal protein and is used as a reference line. The rapid detection test paper bar has high specificity, can carry out semi quantitative detection, can be used at the environmental temperature of 4 to 40 DEG C, and is suitable for an individual farmer, a food hygiene and quality inspection department, customs and other animal source food to carry out rapid detection on spectinomycin residues. The spectinomycin colloidal gold detection card provided by the invention has the advantages of high specificity, high sensitivity, simple and convenient operation and the like, and can carry out semi quantitative detection.

The invention discloses a measuring method for detecting residual quantities of streptomycin, dihydrostreptomycin, kanamycin, spectinomycin and neomycin in food simultaneously. The method comprises steps as follows: (1) extraction; (2) purification; (3) preparation of a matrix standard working solution; (4) measurement with LC-MS / MS (liquid chromatography-tandem mass spectrometry). The method has the advantages of simplicity in operation, accuracy, high sensitivity and good repeatability, can completely meet technical requirements for corresponding product security detection in China, the European Union, America and Japan and can provide powerful technical support for guaranteeing food security of people in China and sound development of export trades.

The invention provides high resolution X-ray crystal structures of the 30S ribosome, obtained from Thermus thermophilus 30S subunit, having a tetragonal space group P41212 to which are bound an antibiotic selected from the group paromomycin, streptomycin, spectinomycin, tetracycline, pactamycin and hygromycin B. An advantageous feature of the structure is that it diffracts at about 3 Å resolution. The invention also provides a crystal of 30S having the three dimensional atomic coordinates of the 30S ribosome, the coordinates being provided in any one of tables 1 to 4. The data may be used for the rational design and modelling of inhibitors for the 30S ribosome, which have potential use as antibiotics.

The invention belongs to the technical field of medical dressings for repairing burn skin wounds, and provides a double-sustained-release drug-loaded hydrogel dressing with double-layer microspheres entrapped by a semi-interpenetrating network. Acrylic acid is used as a pH-sensitive monomer. The pH / temperature dual-sensitive intelligent hydrogel takes methacrylic acid 2-ethyl ester and oligomeric (ethylene glycol) methyl ether methacrylate as temperature-sensitive monomers, and an anti-inflammatory drug gentamicin sulfate is entrapped; the preparation method comprises the following steps: preparing a calcium alginate core sphere entrapped with bovine serum albumin through a high-voltage electrostatic instillation method, coating the surface of the core sphere with a chitosan shell layer entrapped with an antibacterial drug azithromycin to form a double-layer microsphere, and loading the double-layer microsphere into the hydrogel to obtain the hydrogel dressing capable of precisely and slowly releasing two drugs and one protein. According to the pH / temperature change of a wound surface microenvironment, the hydrogel can achieve accurate controlled release of drugs and improve the utilization rate of the drugs, and is a novel double-sustained-release drug-loaded hydrogel dressing with a semi-interpenetrating network combined double-layer microsphere structure and good comprehensive performance.

The invention discloses a dilute solution used for preserving racoon dog seminal fluid at normal temperature, and a semen collection method. The dilute solution comprises following ingredients: 36 to 38g of glucose, 1.20 to 1.30g of sodium bicarbonate, 1.20 to 1.30g of ethylene diamine tetraacetic acid, 5.50 to 6.30g of sodium citrate, 0.65 to 0.85g of potassium chloride, 0.50 to 3.05g of bovine serum albumin, 0.05 to 0.30g of acetylcysteine, 0.50 to 1.20g of penicillin, 0.30 to 1.00g of streptomycin, 0.05 to 1.50g of lincomycin and 0.01 to 0.05g of spectinomycin; and a mixture of the ingredients above is diluted to 1000ml by adding triple-distilled water so as to obtain the dilute solution. Key steps of the semen collection method are that: female racoon dogs in heat are used as dummy teaser; conditioned reflex of male racoon dogs to artificial semen collection is formed by training; the training is repeated for 3 to 4 times until conditioned reflex is formed; and then the female racoon dogs are replaced by a female racoon dog-simulated dummy teaser which is filled with urine of female racoon dogs in heat so as to collect male racoon dog semen. The dilute solution and the semen collection method can be used for collecting racoon dog semen, diluting for preservation, realizing artificial insemination and obtaining offspring; and are capable of promoting the popularization of racoon dog artificial insemination technologies, and increasing economic benefits of racoon dog industry.

Belonging to the technical field of enzyme genetic engineering, the invention relates to a Cre / lox system based method for construction of resistance gene free chromosome integrated recombinant bacillus subtilis expressing D-psicose 3-epimerase. The method includes: taking integration vector pDGIEF as the starting vector, deleting the original spectinomycin (spc) antibiotics resistance gene, and introducing a new antibiotics resistance gene fragment of lox71-spc-lox66 to obtain plasmid pDGI-7S6; fusing a P43 promoter at the upstream of the DPE enzyme gene of ATCC 35704 to construct P43-DPE, constructing the P43-DPE into pDGI-7S6 to obtain pDGI-7S6-P43-DPE; then converting 1A751 competence, under the action of amylase homologous arm, integrating P43-DPE and lox71-spc-lox66 to 1A751 chromosome; utilizing the Cre / lox system to knock out the spc resistance gene, thus obtaining a food grade safe 1A751 / lox-DPE strain producing D-psicose 3-epimerase. The strain is named as Bacillus subtilis SK38.002 with a preservation number of CCTCC NO:M 2015258, has fermentation broth total enzyme activity up to 6U / mL, and has important industrial application value.

The invention discloses a method for detecting the aminoglycoside antibiotic residue amount in an animal-derived food and particularly relates to a method for detecting the aminoglycoside antibiotic residue amount in the animal-derived food based on HILIC chromatographic column tandem triple quadrupole mass spectrometry. The method is characterized in that a sample is subjected to extraction, solid phase extraction and ZIC-HILIC chromatographic column separation and then is determined through quadrupole mass spectrometry. The detected neomycin, spectinomycin, dihydrostreptomycin, streptomycin and gentamicin concentrations have good liner relation within the range of 10-500 micro-g / kg, a response value S / N under the concentration of 20 micro-g / kg is good and is greater than 10, the sample truth degree has no obvious matrix effect, and the detection limit of the concentration in the sample can be reduced to be 100 micro-g / kg. Compared with the prior art, the method has the advantages of being short in sample earlier-stage treatment time, low in cost, simple, convenient, comprehensive in matrix coverage, high in accuracy and the like.

The invention provides an azithromycin lactobionate hydrate and the preparation and application thereof. The molecular formula of the azithromycin lactobionate hydrate is C38H72N2O12.2C12H22O12.nH2O, wherein n is equal to between 2.0 and 5.5, the hydrate has good water solubility and storage stability and is applicable to the treatment and prevention of human or animal infectious diseases caused by sensitive bacterium of gram-positive or-negative bacterium.