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Diluent formula for improving frozen quality of yak frozen semen

A technology of freezing semen and diluent, which is applied in the field of agricultural livestock, can solve the problems affecting artificial insemination, low vitality, low fertilization rate, etc., and achieve the effect of improving integrity, improving quality, improving sperm motility and acrosome integrity

Inactive Publication Date: 2019-01-25
QINGHAI UNIVERSITY +1
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Problems solved by technology

However, in production practice, there are often cases of low vitality and low fertilization rate after freezing and thawing sperm, which affects the effect of artificial insemination

Method used

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  • Diluent formula for improving frozen quality of yak frozen semen

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Embodiment Construction

[0015] The present invention will be further described below:

[0016] According to the technical scheme of the present invention, the substances needed to prepare 100 ml of frozen semen dilution are: sodium citrate: 0.9-1.2 grams, sucrose: 3-5 grams, low-density lipoprotein: 7-9 mg, glycerol: 4-5 grams 7 ml, resveratrol: 6-8 mg, melatonin: 5-7 mg, spectinomycin: 35 mg, gentamicin: 80,000 units (IU).

[0017] Its best formula for frozen semen dilution is: the content of substances per 100 ml is: sodium citrate: 1.073 mg, sucrose: 4.344 mg, low-density lipoprotein: 8 mg, glycerin: 6 ml, resveratrol : 7 mg, melatonin: 5 mg, spectinomycin: 35 mg, gentamicin 80,000 units (IU).

[0018] Its preparation method is:

[0019] 1) Weigh the raw materials in the formula: sodium citrate, sucrose, low-density lipoprotein, glycerin, resveratrol, melatonin, distilled water.

[0020] 2) Prepare the above sodium citrate, sucrose, low-density lipoprotein, melatonin, resveratrol, glycerin, and...

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Abstract

The invention discloses a diluent formula for improving the frozen quality of yak frozen semen. Each 100 milliliters of frozen semen diluent is prepared from 0.9 to 1.2 grams of sodium citrate, 3 to 5grams of saccharose, 6 to 8 milligrams of low density lipoprotein, 4 to 7 milliliters of glycerinum, 6 to 8 milligrams of resveratrol, 5 to 8 milligrams of melatonin, 35 milligrams of spectinomycin and 80 thousand units of gentamicin. According to the diluent formula disclosed by the invention, the low density lipoprotein, the resveratrol and the melatonin are jointly added into a semen diluent for the first time, and a diluent preparation method is corrected. Experimental data show that the frozen semen diluent not only can improve sperm motility and acrosomal intactness rate of frozen-thawed semen, but also improves activity of catalase (CAT), glutathione peroxidase (GR) and glutathione reductase (GSH) in seminal plasma which are used for protecting sperm fertilizing ability; furthermore, sperm DNA integrity is improved, and the fact that the semen formula can improve the frozen quality of semen is ensured.

Description

technical field [0001] The invention relates to the technical field of agricultural livestock, in particular to a diluent formula for improving the frozen quality of yak frozen semen. Background technique [0002] The successful freezing of domestic animal semen has had a profound impact on the development of artificial insemination technology. Firstly, it has laid the foundation for expanding the utilization rate and prolonging the utilization time of excellent breeding males, and secondly, it has provided a broad space for the development of artificial insemination and cryobiology. Because frozen semen is more convenient in transportation, no matter in terms of time or space, the mating efficiency of male animals can be fully exerted, and the pace of livestock breed improvement has been accelerated. Although all countries have launched research on frozen semen of cattle, pigs, horses, and chickens, only the artificial insemination technology of frozen bovine semen is widel...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 桂林生韩银仓孙永刚赵宪林
Owner QINGHAI UNIVERSITY
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