Novel bacterial strain for highly effective degradation of chrysanthemum ester and organophosphorus pesticide and uses thereof
An organophosphorus, new strain technology, applied in applications, bacteria, microbe-based methods, etc., can solve problems such as large losses
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Embodiment 1
[0034] Embodiment 1: JCX22D bacterial strain biodegradation permethrin pesticide
[0035] JCX22D was cultured in LB medium until it reached the logarithmic growth phase, and inserted into the basal salt medium with 200 mg / l of beta-cypermethrin, cyhalothrin, deltamethrin and bifenthrin at 2% inoculum, 28 ℃, 180rpm constant temperature oscillator shaking culture, sampling according to a certain culture time, the samples were immediately extracted with cyclohexane, gas chromatography (Trace GC) was used to determine the pesticide residues, and the detector was an electron capture device (ECD). The measurement results are shown in Table 4.
[0036] Table 4 Degradation rate of four pyrethroid pesticides by JCX22D strain
[0037] Types of pesticides
Embodiment 2
[0038] Embodiment 2: JCX22D bacterial strain biodegradation organophosphorus pesticide
[0039] JCX22D was cultured in LB medium to the logarithmic growth phase, and inoculated with phoxim, parathion-methyl, chlorpyrifos and diazinon at 200 mg / l basal salt medium at 28°C. , 180rpm constant temperature culture, sampling according to a certain culture time, cyclohexane extraction, using UV-VIS 8500 double-beam ultraviolet / visible spectrophotometer for wavelength scanning, and comparing the samples with the control to determine the degradation rate of pesticides. The measurement results are shown in Table 5.
[0040] Table 5 Degradation rate (%) of JCX22D bacterial strain to four kinds of organophosphorus pesticides
[0041] 24hr
Embodiment 3
[0042] Embodiment 3: JCX22D bacterial strain biodegrades abamectin
[0043] JCX22D was cultured in LB medium until it reached the logarithmic growth phase. Inoculate 2% of the inoculum into the basal salt medium with 200 mg / l abamectin, culture at 28°C and 180rpm at constant temperature, and take samples according to a certain culture time. Chloroform extraction, use UV-VIS 8500 double-beam ultraviolet / visible spectrophotometer for wavelength scanning, and determine the degradation rate of pesticides by comparing the samples with the control. The measurement results are shown in Table 5.
[0044] Table 5 JCX22D bacterial strain is to the degradation rate (%) of abamectin
[0045]
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