63 results about "Parathion methyl" patented technology

The invention discloses a parathion-methyl detection method which comprises the following steps: preparing a n-doped carbon quantum dot (N-CDs) by using a hydrothermal method; decomposing parathion-methyl under an alkali condition to generate paranitrophenol; mixing the prepared N-CDs with a hydrolysis product, namely the paranitrophenol; and enabling the paranitrophenol to absorb fluorescence ofthe N-CDs to quench the fluorescence of the N-CDs, and detecting the concentration of the parathion-methyl according to fluorescence intensity. In the method, sample pretreatment is avoided, large-size instrument or enzyme is not needed, the detection method is simple to operate, high in detection specificity, free of interference of other common pesticides, high in detection sensitivity, low in detection limit, high in stability and wide in application, and can be applied to detection on parathion-methyl in fruits, vegetables, river samples, and the like, and the cost can be greatly reduced.The method has good application prospects and potential application values in the fields of detection and analysis.

The invention discloses a parathion-methyl extraction and detection method based on an N-octylpyridinium hexafluorophosphate electrode. The method comprises the following steps: immersing the N-octylpyridinium hexafluorophosphate electrode into a sample solution, and directly extracting and enriching parathion-methyl; after the extraction and enrichment are finished, with the N-octylpyridinium hexafluorophosphate electrode as a working electrode, a saturated Ag / AgCl electrode as a reference electrode and a platinum filament as an auxiliary electrode, detecting the parathion-methyl through an electrochemical cyclic voltammetry or a linear scanning method. According to the method, the sample pretreatment is greatly simplified, and the pesticide is directly extracted, concentrated and detected on the electrode. Furthermore, the whole electrode is formed by a uniform mixture, the structure is firm, and film falling problem is avoided. In addition, a new surface can be obtained by slightly polishing the electrode with weighting paper, so that measurement can be performed.

The invention relates to an organophosphorus pesticide degradation enzyme obtained through genetic engineering reconstruction. A random mutation reconstructed organophosphorus pesticide degradation enzyme is generated by the fact that three amino acid loci of an organophosphorus pesticide degradation enzyme are derived from pseudomonas stutzeri. The replaced amino acid loci are a 31st locus, a 124th locus and a 246th locus. The organophosphorus pesticide degradation enzyme is good in degradation activity for chlorpyrifos and parathion-methyl, and also has good heat stability.

The invention discloses a method for simultaneously detecting parathion-methyl, chlorpyrifos and trichlorfon based on quantum dot labeled biomimetic fluorescence immunoassay. According to the invention, the multi-recognition-site Fe3O4@SiO2@MIP is used as the common biomimetic antibody, and three organophosphorus pesticide haptens such as parathion-methyl, chlorpyrifos and trichlorfon are respectively labeled with three kinds of quantum dots with different emission wavelengths at the same excitation wavelength, such that the simple and rapid biomimetic fluorescence immunoassay method for simultaneously detecting the three organophosphorus pesticides such as parathion-methyl, chlorpyrifos and trichlorfon is established; and the method has advantages of low cost, simple experiment operation,accurate measurement result and low detection limit, and is suitable for the simultaneous detection of various organophosphorus pesticides in various foods.

The invention provides enterobacter bacteria for efficiently degrading organophosphorus pesticides and a culturing method thereof. The enterobacter bacteria are characterized in that the main biological property of the enterobacter bacteria is Gram-negative, thalli are in a short-rod shape without spores, and the size is 0.6-1.1*1.2-2.5 mum; and the enterobacter bacteria have single-end overgrowing flagella and are aerobic bacteria, the optimum growing temperature is 30-37 DEG C, the optimum pH value is 7-8, the enterobacter bacteria form a bacterial colony with the diameter of 1-2 mm after being cultured on a blood agar medium for 48 h, and the bacterial colony is circular in shape and yellow in color, has no transparence, knurls, trim edge and moist and glossy surface. The Genbank login number of the bacterial strain 16SrDNA is GQ380575. The tolerance concentration of the bacterial strain on parathion-methyl and Dursban can reach 5000 mg / L in an inorganic salt medium containing 1% of cane sugar, the tolerance concentration on dimethoate reaches 10000 mg / L, the tolerance capacity of the bacterial strain is furthest higher than that of agricultural dose used in agricultural production, the degradation rate for the organophosphorus pesticides in soil reaches above 90%, and the enterobacter bacteria solve the problem that pesticide residues in the agricultural production exceed the standard, and can be used for producing green and nuisanceless agricultural products.

The invention discloses a pest repellent and a preparation method thereof and relates to the field of crop protection. The pest repellent comprises, by weight parts, 10-20 parts of methyl-isp, 5-10 parts of parathion-methyl, 2-6 parts of tributyl tin chloride, 7-12 parts of cyclohexanone, 5-8 parts of isobutyl cyanide, 2-4 parts of cyclodextrin, 3-8 parts of methylbenzene, 1-2 parts of carbomer, 2-3 parts of ethanol with a concentration of 95%, 7-9 parts of polyethylene glycol, 0.5-1 part of triethanolamine, 7-10 parts of prickly ash ethanol extract and 4-6 parts of water hyacinth ethanol extract. By adoption of the technical scheme, the method is reasonable in preparation steps and convenient to operate, and the prepared pest repellent is non-toxic and harmless and has a certain promotion effect of plant growth while having high repelling performance.

The invention discloses preparation and application for an electrochemical sensor based on a nano-structure boron-doped diamond electrode, in particular relates to a preparation method for a nano-structure boron-doped diamond electrode and a method for the nano-structure boron-doped diamond electrode in rapid detection on organophosphorus pesticide residues, and aims at solving the technical problems of low electrocatalytic activity, difficult surface re-construction, and poor selectivity and sensitivity of a boron-doped diamond (BDD) electrode. The method disclosed by the invention comprises the following steps (1) pre-depositing a layer of gold nanodots on a boron-doped diamond electrode as a mask; (2) etching the morphology of a nano-structure on the boron-doped diamond surface in an Ar / H2 atmosphere; and (3) etching the boron-doped diamond surface, and cooling to obtain the nano-structure boron-doped diamond electrode. According to the preparation and the application disclosed by the invention after the technical scheme is adopted, the performance of the electrode is improved, the sensitivity of the electrode is improved, and the electrode is directly applied to detection on the organophosphorus pesticide residues with electrochemical characteristics, such as parathion and parathion-methyl.

The invention relates to ultralow-volume liquid containing lufenuron and organic phosphorus insecticide, which comprises chemical composition, surfactant and solvent. The chemical composition is composed of lufenuron and an active ingredient II. The active ingredient II can be optional one of fenitrothion, dipterex, parathion-methyl, isazofos, pyridaphenthion, quinalphos, triazophos, colophonate, phoxim, fenitrothion, pirimiphos-methyl, fenthion, cyanophos, profenofos, chlorpyrifos-methyl, prothiofos, sulprofos, dimethoate, malathion, methidathion and isocarbophos. The ultralow-volume liquid is convenient in process, long-acting and highly efficient, targets are less apt to resist the ultralow-volume liquid, environment pollution is low, insecticide and water are economized, and insecticide utilization rate is increased. The ultralow-volume liquid can be used for controlling various pests for rice, wheat, corn, vegetables, tobacco, tree trees, fruit trees and sugarcane by ultralow-low spraying, low-volume spraying or ultralow-volume electrostatic spraying.

The invention discloses a film assembled from gold nanoparticle, as well as a preparation method and application of the film. A plurality of layers of the gold nanoparticles are mutually adhered on a conducting substrate so as to form the film of which the thickness is 200nm-2<mu>m, wherein the grain diameter of the gold nanoparticles is 30-120nm; and the clearance or gap between every two gold nanoparticles is equal to or smaller than 10nm. The method comprises the following steps of: sputtering a gold film on the surface of the conducting substrate so as to obtain the conducting substrate of which the surface is covered with the gold film; placing the conducting substrate in gold electrolyte by using a graphite flake as anode and using the conducting substrate of which the surface is covered with the gold film as a cathode; and performing electro-deposition under constant current to obtain the target product. The film is high in SERS activity, and is extremely easy to widely and commercially apply to rapid trace detection for parathion-methyl of pesticides.