84results about How to "Improve secretion capacity" patented technology

The invention relates to the field of medicines, and in particular to compound probiotic, application thereof for treating anaphylactic diseases and allergy free probiotic electuary for pregnant and lying-in women. The compound probiotic consists of bifidobacterium Bi-07, lactobacillus rhamnosus LR22, lactobacillus acidophilus NCFM. The compound probiotic not only can irritate secretion of IFN-gamma and IL-2 in animal body, but also inhibit secretion of immune globulin E (IgE) and IL-4 so as to prevent and treat anaphylactic diseases. The invention further provides a medical preparation comprising the compound probiotic and allergy free probiotic electuary for pregnant and lying-in women. The pharmacodynamic test shows that the medical preparation has remarkable effect of improving IL-2 and IFN-gamma. In addition, compared with the control group, the content of IgE in the test group is further remarkably reduced. Long-term clinical tests show that the medical preparation comprising the compound probiotic provided by the invention is free from untoward effects to human body.

The invention provides a novel coronavirus vaccine and a preparation method and application thereof. The encoding gene of the vaccine comprises a peptide fragment sequence, a Kozak sequence, an IgG signal peptide sequence and a human IgG Fc structural domain sequence, wherein the peptide fragment sequence contains the novel coronavirus and is combined with ACE2 in an S protein receptor binding domain RBD; the Kozak sequence and the IgG signal peptide sequence are positioned at the 5 'end of the peptide fragment sequence combined with ACE2 in the RBD; and the human IgG Fc structural domain sequence is positioned at the 3' end of the peptide fragment sequence combined with ACE2 in the RBD. The novel coronavirus vaccine has the advantages that the 1168 to 1569 sites (containing the peptide fragment combined with ACE2 in the RBD) of the encoding sequence from S protein are used as the gene sequence of antigen protein, and are modified, so that the expression ability, secretion ability and immunogenicity of the antigen protein are improved; the prepared DNA vaccine, protein vaccine and mRNA vaccine are directly injected into a human body or are injected into the human body afterbeing packaged by an adjuvant to express the corresponding antigen, and the body is induced to generate immune response; and the broad application prospect is realized in the fields of COVID-19 prevention and treatment.

The invention provides an insulin aspart precursor. The amino acid sequence is Z1(XY)nZ2Z3Z4-B(1-29)-C1C2C3-A(1-21), wherein Z1, X and Z2 are Asp or Glu respectively, Y is Ala or Gly, n is an integer from 1-10, Z3 is Pro, Glu or Asp, Z4 and C3 are Lys or Arg respectively, C1 and C2 are Glu, Asp, Ala or Gly respectively, B(1-29) is insulin aspart B chain 1-29 bit amino acid, and A(1-21) is insulin aspart A chain 1-21 bit amino acid. The invention also provides a coding gene, a cloning vector or an expression vector containing the coding gene, a transformant, and a method for preparing quick-acting insulin by utilization of the quick-acting insulin aspart precursor protein. The expression level of the product is high, the fermentation expression product purity is high, the extraction method is simple and the yield is high.

The invention provides a feed formulation used for lactation period of female Rex Rabbit; according to a certain proportion, the feed formulation consists of peanut vine powder, sweet potato vine powder, corn, bran, soybean meal, peanut meal, edible salt, calcium hydrogen phosphate, composite pre-mixing material, methionine, lysine, necoxine, compound vitamin, trace elements and alfalfa extract. On the basis of nutrition matching, the feed formulation uses compound vitamin and bioactive substance, increases part normally used medicines, is extremely effective for the control and prevention of normal diseases and is beneficial for reinforcing the immunities and milk yield of the female Rex Rabbit.

The invention relates to a preparation method of blueberry and roselle composite instant-dissolvable powder including prebiotics. The preparation method includes following steps: preparing pulp, performing enzymolysis, preparing juice, preparing roselle juice, performing a mixing process and a concentrating process, adding the prebiotics, and performing filtration, a freezing process and a packaging process. In the invention, an instant-dissolvable fruit powder is prepared with cooperative use of the three healthy foods: blueberry, roselle and the prebiotics, so that the instant-dissolvable powder is richer in mouthfeel and nutritional value. Organic acids in the blueberry fruits and roselle calyxes have antibiotic effects and have functions of reducing a pH value of digested substances and improving secretion of pancreas. Fructo-oligosaccharide is a natural active substance and is a novel sweetening agent which has health-caring functions of regulating intestinal flora, reproducing bifidobacterium, promoting absorption of calcium, regulating blood fat and immune system, resisting dental caries and the like. During the preparation process, by means of the preparation method, damages on vitamins and organic substances are reduced as far as possible so that nutritional value of the instant-dissolvable powder is ensured to loss as less as possible. The instant-dissolvable powder can be dissolved in water at low temperature and is wider in application.

The invention provides an umbilical cord mesenchymal stem cell factor freeze-dried powder and a preparation method thereof. Umbilical cord mesenchymal stem cells are cultured by mechanical stimulationand hypoxia stimulation to promote secretion of stem cell factors, and reasonable combination of components such as skimmed milk powder, trehalose, carboxymethyl cellulose, hydroxyethyl starch, glutamine and poloxamer is used as a freeze-dried powder protective agent. The prepared expected mesenchymal stem cell factor freeze-dried powder has good stability, stem cell factor content and activity.

The invention belongs to the technical field of microbial biology, and relates to a high-throughput pichia pastoris screening method based on a droplet micro-fluidic chip. The screening method comprises the following steps: step 1, constructing pichia pastoris; step 2, constructing a pichia pastoris mutant library; step 3, carrying out single-droplet embedding and droplet culture on strains in thePichia pastoris mutant library to obtain droplets containing Pichia pastoris mutants; step 4, carrying out droplet microfluidic sorting according to the intensity of fluorescence in the droplets, andscreening out the droplets; and 5, respectively carrying out shake-flask fermentation and re-screening on the droplets to obtain a pichia pastoris strain with improved xylanase secretion capacity. The pichia pastoris strain of xylanase fused fluorescent protein is screened by adopting a droplet microfluidic technology, and the screening flux can reach 100 thousand strains per hour; by using the method, for screening of a million-level strain library, only 10 hours are needed, and the total volume of consumed fluorescent reagents is 100 microliters.

The invention discloses a humanized antibody or antibody fragment for PD-L1 extracellular fragments and application thereof, a nucleotide sequence and a carrier, belongs to the technical field of antibody humanization. The antibody or the antibody fragment comprises a heavy chain and a light chain. Each of the heavy chain and the light chain comprises a variable region. Each variable region comprises complementary determining regions. The complementary determining regions CDR1, CDR2 and CDR3 of the heavy chain are represented by HCDR1, HCDR2 and HCDR3 respectively. The complementary determining regions CDR1, CDR2 and CDR3 of the light chain are represented by LCDR1, LCDR2 and LCDR3 respectively. The antibody or the antibody fragment can improve immunity systems and promote lymphocyte to secrete interleukin-2, and is applied to preparation of diagnostic reagents and anti-tumor, anti-inflammation and anti-contagion drugs.

The invention belongs to the technical field of microorganisms and particularly relates to high-conversion-rate bacillus subtilis and preparation and application thereof. The problem that the bacillus subtilis A164 is low in conversion efficiency is solved through a plasmid expression method, then a linear fragment containing a comk expression cassette is integrated on an A164 genome through an induced conversion method, and resistance is eliminated by the aid of a cre / lox specific recombination system to obtain bacillus subtilis A164S which is free of resistance marker, easy to operate and high in conversion efficiency and secretion capability. Research results show that conversion efficiency of the bacillus subtilis A164S is more than 1000 times of that of the bacillus subtilis A164; the bacillus subtilis A164S can effectively take in homologous DNA, can efficiently secrete heterologous proteins, and has high application potential in recombinant expression of exogenous genes.

The invention discloses a preparing method for high-toxicity human Vgamma9Vdelta2 T cells induced by a PD-1 antibody. The preparing method comprises the following steps that firstly, human peripheral blood mononuclear cells are obtained through saccharosan-glucosamine diatrizoate density gradient centrifugation; secondly, separated PBMCs are put into an RPMI1640 culture medium containing an irritant mycobacterium tuberculosis heat-resistant antigen, cell factors rhIL-2 and autologous plasma accounting for 5-10%; rhIL-12 and the PD-1 antibody are added at the same time to culture PBMCs; thirdly, the fresh RPMI1649 culture medium is supplemented every other 2-3 days according to the growing condition of the cells; fourthly, the cells are collected on the 10th day to the 16th day according to the growing condition of the cells, and the high-toxicity human Vgamma9Vdelta2 T cells are obtained. The preparing method for the Vgamma9Vdelta2 T cells which are fast in cell proliferation, high in cell purity, high in cell toxicity, strong in tumor damage capability and low in cost is provided, and a basis for clinical application of the Vgamma9Vdelta2 T cells on tumor treatment medicine is laid.

The invention provides a production technique for comb foundation-free honey of Apis cerana. The production technique comprises the following steps: manufacturing a beehive with two queen bees in one beehive; replacing comb honey bee lattices in one month before each blooming period, feeding honey bees with a nutritional feed in 5-30 days before the blooming period, adopting sealing plates to seal comb honey bee nests on the comb honey bee lattices during the process, and timely taking out to place into a sterilized thermostat container for storage stopping feeding the honey bees with the nutritional feed within 5 days before the blooming period; when the blooming period begins, examining the sealing condition of the comb honey bees at any time, and replacing the comb honey bee lattices provided with sealed comb honey by the comb honey bee lattices stored in the thermostat container. The production technique realizes two queen bees in one beehive through the unique beehive, and increases the amount of worker bees in the beehive, so that the speed of building nests and collecting honey of the Apis cerana is higher; meanwhile, by feeding the Apis cerana with the nutritional feed, integral health of the honey bees is improved, the secretion capability of the worker bees is enhanced, and the speed of building nests is accelerated; by the adoption of the production technique, the speed of producing comb honey is high, and the amount of comb honey is increased.

The invention provides a kluyveromyces marxianus with a preservation number of CGMCC No. 10621, and also provides the application of the kluyveromyces marxianus. Compared with existing kluyveromyces marxious such as ATCC8585 and ATCC26548, the kluyveromyces marxianus has higher protein secretion capacity and higher biomass, can be used for producing enzymic preparations, and also can be used for building a recombinant protein expression system.

The invention discloses a skin care composition for controlling oil and repairing a skin barrier as well as a preparation method and application of the skin care composition. The skin care composition is prepared from the following components: saccharide isomeride, rutin and hibiscus sabdariffa flower fermentation product filtrate in a ratio of (1-5): (1-5): (1-5). The composition can be used for instantly and efficiently adsorbing grease by utilizing the saccharide isomeride, reducing excessive grease secretion of sebaceous glands in a long acting manner by utilizingthe saccharide isomeride and rutin, and moistening and repairing the skin barrier and regulating moisture-oil balance by utilizing the hibiscus sabdariffa flower fermentation product filtrate, so that the problem of facial grease production caused by excessive grease secretion of sebaceous glands is improved.

The invention provides adipose-derived mesenchymal stem cell factor lyophilized powder and a preparation method thereof. According to the method, an adipose-derived mesenchymal stem cell is cultured by adopting conditions of mechanical stimulation and hypoxic stimulation, the secretion of stem cell factors is promoted, the reasonable cooperation of polyethylene glycol, konjak sugar, proline, glutathione, sodium benzoate, sodium dodecyl sulfate and tetrahydro-2-pyrimidinone is adopted to serve as a lyophilized powder protective agent, and the prepared adipose-derived mesenchymal stem cell factor lyophilized powder has good stability, stem cell factor content and activity.

The invention discloses an intercropping feed and intercropping method for grass carps, carps, crucian carps and variegated and white carps. The intercropping feed is prepared from the following raw materials in parts by weight: 900-950 parts of a basic coarse feed, 10 parts of soybean phospholipid oil, 10 parts of soybean oil, 3 parts of sodium chloride, 10-11 parts of zeolite powder, 20 parts ofcalcium dihydrogen phosphate, 3 parts of choline chloride, 1 or less of part of Gandankang, and 10 parts of a composite premix feed for fish. According to the intercropping feed and the intercroppingmethod disclosed by the invention, physiological habits and characteristics of the grass carps, the carps, the crucian carps and the variegated and white carps are sufficiently considered, the grasscarps are mainly raised, and the intercropping feed can concurrently raise other kinds. Primary breeding test proves that components in the feed formula can generate good synergistic effects, the fishdiseases caused by factors of nutrients, environment, bacteria and the like can be effectively prevented, and the status that when being drawn in the net, the carps bleed and the carps are not resistant to transport, can be notably improved. The intercropping feed has the advantages of improving the feed digestibility, promoting growth, reducing the feed coefficient by 0.18 or above and the like,the growth rate, the daily gain and the survival rate of various intercropped fish can be effectively increased, the whole yield can be increased, and the economic benefits can be increased.

The invention discloses a composite feed capable of protecting the liver health of tilapia. The composite feed is obtained by mixing Chinese herbal medicine extracts and basic ingredients, and drying the mixture, wherein the weight ratio of the basic ingredients to the Chinese herbal medicine extracts is (995 to 999): (1-5); each 1 to 5 L of the Chinese herbal medicine extracts is obtained by conducting water extraction on the following Chinese herbal medicine: 2 to 10 g of panax notoginseng, 3 to 15 g of shizandra berry, 5 to 20 g of astragalus membranaceus,5 to 15 g of liquorice, 2 to 10 g of radix notoginseng, 6 to 18 g of lithospermum, 5 to 15 g of silybum marianum, and 2 to 10 g of fructus psoraleae. The composite feed is used for tilapia culture, can be used for protecting the liver health of tilapia, and improves the non-specific immunity of the organism of tilapia. The invention further provides a preparation method of the composite feed.

The invention belongs to the technical field of immunotherapy, and particularly relates to application of a hypoxia-adjustable promoter in CAR-T, a CAR structure of the hypoxia-adjustable promoter, anexpression vector, CAR-T cells, application of the CAR-T cells, a method for improving the IFN-gamma and / or IL-2 factor secretion capacity of the CAR-T cells in a hypoxia environment, and a method for improving the CAR-T cell killing capacity in the hypoxia environment. The CAR structure contains the hypoxia-adjustable promoter, the hypoxia-adjustable promoter is formed by connecting Hif1a adjusting elements and mini promoters, and the number of repetitions of the Hif1a adjusting elements is 3-5. The CAR structure induced and started by the hypoxia microenvironment not only can be effectivelyexpressed in T lymphocytes, but also can enhance the activation of the CAR-T cells in the hypoxia environment and enhance the effectiveness and safety of the CAR-T, and can be used for targeted therapy of tumors.

The invention discloses a method for producing 1, 3 propylene glycol safely and high efficiently, i.e. knocking out the wzi gene, which causes the generation of capsules, from K.pneumoniae through a specifically improved Red recombination system to improve the security and product secreting performance of 1, 3-PD fermentation. Compared with the prior art, the method of the invention substantially raises the gene knockout efficiency of the K.pneumoniae. The K.pneumoniae having wzi capsule gene deletion has a high product concentration, great production intensity and high security.

The invention relates to the field of genetic engineering, and particularly discloses a set of system for evaluating whether CHO expression and secretion capacities are influenced after CHO cells are subjected to gene modification. The expression system includes a eukaryotic expression vector and a CHO expression evaluating cell strain. The eukaryotic expression vector and the stable cell strain include a piggy transposon regulating and control unit, a screened gene expression unit and an antibody-LucGFP fused reporter gene expression unit. The expression system can achieve site-specific integration and efficient expression of an exogenous gene in the CHO cell genome. The system can be used for evaluating influences of any exogenous DNA segment including genes, RNAs and the like on the secretion function of the CHO cells, and has a wide application prospect in the field of modifying the CHO cells to increase the protein yield.

The invention provides a culture method of amorphous pearls. The method comprises the following steps of: (1) cutting off edge membranes of freshwater mussels or pearl oysters, cutting off color lines, taking pallium exocuticles and putting the pallium exocuticles in a culture dish containing a Hanks liquid; (2) taking the pallium exocuticles out of the culture dish and mashing the pallium exocuticles for 3-5 minutes to obtain pulp; (3) sucking the pulp into a centrifuge tube, and naturally settling for 3-5 minutes to obtain a suspending liquid; (4) transferring the suspending liquid into another centrifuge tube and removing larger tissue blocks to obtain the final suspending liquid for layer use; and (5) pumping the suspending liquid by using an injector and injecting the suspending liquid into palliums or visceral masses of the freshwater mussels or pearl oysters by using the injector. In the production process of non-nucleated pearls, the culture method has the advantages that the wound is small; the wound can be healed easily and the survival rate of the freshwater mussels or pearl oysters is high; in the production process of nucleated pearls, the problems of uneven flakelet wrapping, uneven nacre secretion or flakelet falling off can be overcome; and the secretion capability of nacre of the freshwater mussels or pearl oysters can be improved greatly and the production cycle can be reduced by more than 20%, therefore, the culture method has the advantage of high beneficial and practical value.

The invention provides compound feed capable of improving intestinal tract health of channel catfish. The compound feed is prepared by mixing and drying a Chinese herbal extract and a basic component, wherein the weight ratio of the basic component to the Chinese herbal extract is (995-998): (2-5); each 2-5kg of Chinese herbal extract is prepared from the following Chinese herbal components through water extraction: 5-15g of pseudoginseng, 10-25g of dandelion, 10-30g of astragalus membranaceus, 10-25g of coptis chinensis, 10-30g of liquorice, 10-25g of perila seed, 10-30g of rheum officinale and 20-50g of garlic. The compound feed can improve health of the intestinal tract and a liver tissue, improve the intestinal immune function, maintain the stability of intestinal flora and prevent intussusception of the channel catfish; meanwhile, the invention further provides the compound feed prepared by the preparation method. The invention relates to the technical field of aquatic feed.

The invention provides a mucosal adjuvant. The mucosal adjuvant is prepared by copolymerization crosslinking compounding of oligo-chitosan and lymphotactin encoding plasmids. The invention also discloses a preparation method and a use of the mucosal adjuvant. When the mucosal adjuvant and a gene vaccine composed of chitosan or oligo-chitosan are synchronously dropped into a nasal cavity for immunization, coxsackievirus specific serum antibody and systemic (spleen and lymph glands) Th1-type immune responses are induced and especially, intestinal mucosa partially-reinforced specific-secretion-type sIgA and IFNgama+Th1 responses are induced, and thus a gene vaccine used with the mucosal adjuvant is obviously superior to an exposed gene vaccine and effectively prevents coxsackievirus-caused myocarditis. The mucosal adjuvant can be used as a novel mucosal vaccination adjuvant.

The drink capable of treating xerophthalmia and alacrimia is prepared with frosted mulberry leaf, chrysanthemum, black bean, cassia see and honey, and through decocting the first four kinds of Chinese medicinal materials, filtering to eliminate medicine dregs and compounding while adding honey. The present invention is prepared through combining the medicinal food materials mulberry leaf, chrysanthemum, black bean and cassia see with eye disease treating function to generate synergistic effect and adding honey to season and replenish nutrients and to reinforce the secretion function of tear gland, so that the present invention can reach the aim of treating xerophthalmia and alacrimia.

The invention relates to application of stromal vascular fraction cells and mesenchymal progenitor cells to the prevention or treatment of osteoarthritis. Specifically, remarkable prevention or treatment effects on osteoarthritis are achieved by applying a medicinal composition containing the stromal vascular fraction cells and the mesenchymal progenitor cells from autologous fat to an object with requirements. The mesenchymal progenitor cells have high cytokine secretion capability and the effect of repairing damage to organisms in a body. The stromal vascular fraction cells and the mesenchymal progenitor cells have chondrogenic differentiation and bone formation capability. The invention also provides the medicinal composition containing the stromal vascular fraction cells and the mesenchymal progenitor cells and a method for preventing and treating osteoarthritis.

The invention discloses boletus speciosus Frost polysaccharide BSF-X as well as a preparation method and an application thereof. The boletus speciosus Frost polysaccharide mainly comprises beta-D-glucose and alpha-D-galactose in the ratio being 2:1, has the average molecular weight of about 141309 Da, has a main chain of (1 arrow 4)-beta-D-glucose, a side chain of arrow 1,6)-alpha-D-galactose connected on 6-O and a arrow 4)-beta-D-glucose connected on galactose 2-O of the side chain. The boletus speciosus Frost polysaccharide has higher immunoregulation activity and can significantly or very significantly promote proliferation of three immune cells including B, T and Raw264.7; BSF-X can promote proliferation of cells, promote macrophage to release immune factors IL-23 and TNF-alpha, has aquite significant inhibition function on L929 in vitro and can inhibit growth of S180 tumor in vivo.

The invention provides a healthcare product for enhancing immunologic function of an organism. The healthcare product comprises the raw materials in parts by weight as follows: 10-20 parts of sea cucumber, 10-20 parts of Astragalus mongholicus, 10-20 parts of Poria cocos, 4-6 parts of Radix Ophiopogonis, 4-6 parts of Fructus Lycii and 1-2 parts of American ginseng. The invention also discloses a preparation method of the healthcare product. According to the invention, the sea cucumber is processed powder conveniently, so defects such as taking inconvenience and carriage inconvenience of the sea cucumber are overcome; and besides, other raw materials are added into the sea cucumber to compound with the sea cucumber, so a function of enhancing the immunologic function of the organism is achieved, problems such as difficult breaking processing of the sea cucumber and high cost are overcome, the processing of the sea cucumber is simplified, the processing difficulty of the sea cucumber is reduced, and the preparation method is more suitable for industrial production.

The invention discloses a preparation method of adipose-derived stem cell multi-cell active factor freeze-dried powder. The preparation method comprises the following steps: S1, collecting adipose tissue of a human body; S2, conducting primary separation, culture and passage of adipose-derived stem cells; S3, carrying out large-scale amplification on mesenchymal stem cells and collecting supernate; S4, preparing a concentrated stock solution of stem cell active factors; and S5, preparation of freeze-dried powder. The multi-cell active factor freeze-dried powder prepared by the preparation method disclosed by the invention includes a large number of cellular active factors, and is safe and reliable to use, free of any adverse reaction, high in yield, good in activity, easy to store and capable of remarkably promoting healing of deep cell tissues of damaged skin and fading scars and acne marks.

The invention provides a compound insulin preparation, which is an oral administration oil preparation prepared by dissolving combined tetrodotoxin and insulin in medical or edible oil, wherein the insulin content is between 0.5 and 10 U / ml, and the tetrodotoxin content is between 0.01 and 10 mu g / ml. In the compound insulin preparation, a trace amount of tetrodotoxin and insulin are combined to form a compound oral administration oil-phase preparation for the defects of the side effect and administration mode of the insulin, so the trouble and flesh bitterness caused by injecting the insulinevery day are prevented, and the side effect of the insulin can be reduced or eliminated.

The invention discloses a preparation method and application of a trophoblast modified by genetic engineering, and aims to research whether K562 cells can stably express IL-21, OX40L and CD48 molecules at the same time and enhance NK cell proliferation and tumor killing ability or not. K562 gene engineering cells simultaneously expressed by the three molecules are used as trophoblasts to culture NK, so that the amplification multiple, purity and killing function of the NK are improved; according to the preparation method, K562 cells can be used for simultaneously and stably expressing membrane binding interleukin-21 (mbIL-21), OX40L molecules and CD48 molecules to prepare an engineering cell strain named as K562-ZHX trophoblast cells, then the engineering cell strain is irradiated with cobalt-60 to obtain trophoblast amplified NK cells, the activity of the NK cells prepared by the method is improved by 3 times, the cell amplification can be improved by more than 300 times, a large amount of time and cost can be saved, and a practical foundation can be laid for obtaining high-purity, high-amplification-multiple and high-killing-activity NK cells.

The present invention relates to a purified aqueous extract material and to a method for preparing same, wherein the purified aqueous extract material includes, as a major ingredient, a glycoside ingredient of Pleuropterus multiflorus and Dipsacus asperoides which have the effect of secreting an insulin-like growth factor and promoting bone structure growth.