44 results about "Coxiella burnetii" patented technology

The invention relates to preparation and application of a rickettsia discrimination detection gene chip. The preparation method comprises the following steps: preparing universal and specific primers, preparing seven rickettsia nucleic acid typing probes, preparing an oligonucleotide chip, establishing a multiplex PCR (polymerase chain reaction) system, and establishing a hybridization system. The gene chip can simultaneously discriminate seven important rickettsiae, including rickettsia prowazeki, rickettsia mooseri, spotted fever group rickettsia, Coxiella burnetii, Orientia tsutsugamushi, Ehrlichia chaffeensis and Anaplasma phagocytophilum. The gene chip has the advantages of high speed, high accuracy, high flux and high specificity, and can provide a novel detection means for clinical diagnosis and epidemiological investigation of rickettsia infection.

The invention discloses a preparation method of a water-soluble carbon dot and application of the water-soluble carbon dot in microbial fermentation, belonging to the technical field of new energy and new materials. According to the preparation method, river crab shell waste is as the raw material, and the acquired carbon dot fluorescence quantum yield can achieve 35% through a simple thermal cracking process. The prepared water-soluble fluorescence carbon dot has biocompatibility, and has an obvious acceleration effect on the fermentation of pneumonia klebsiella pneumonia, the yield of 1,3-propylene glycol can be improved by 30%. Furthermore, the water-soluble fluorescence carbon dot is applied to the standing fermentation of the acetobacter xylinum, the product bacteria cellulose yield can be improved by 75%. The water-soluble fluorescence carbon dot is applied to the microbial fermentation for the first time, and an obvious promotion effect is achieved, and a new growth factor is provided for the microbial fermentation.

The invention provides a method and kit for detecting multiple high-pathogenicity pathogenic bacteria, particularly a method for simultaneously detecting multiple high-pathogenicity pathogenic bacteria, which comprises the following steps: in a polymerase reaction system, carrying out PCR (polymerase chain reaction) on high-pathogenicity pathogenic bacteria by using a specific primer set, thereby obtaining an amplification product; and detecting with a specific probe or probe microsphere. The invention also provides a corresponding kit. The invention can sensitively and conveniently detect and identify multiple high-pathogenicity pathogenic bacteria, including Bacillus anthracis, Yersinia pestis, Clostridium botulinum, Brucella, Streptococcus suis, Vibrio cholerae, Francisella tularensis, Pseudomonas mallei (or Pseudomonas pseudomallei), Coxiella burnetii and Legionella pneumophila.

The invention relates to a method for the detection of prior exposure to Coxiella. burnetii infection by antibody-based assays using recombinant, immunodominant C. burnetii polypeptides. The invention also relates to the design of biotin or His-tagged C. burnetii proteins useful in the antibody-based assays as standardized antigen reagents.

The invention discloses a method for producing 1, 3 propylene glycol safely and high efficiently, i.e. knocking out the wzi gene, which causes the generation of capsules, from K.pneumoniae through a specifically improved Red recombination system to improve the security and product secreting performance of 1, 3-PD fermentation. Compared with the prior art, the method of the invention substantially raises the gene knockout efficiency of the K.pneumoniae. The K.pneumoniae having wzi capsule gene deletion has a high product concentration, great production intensity and high security.

The invention discloses a construction method of 3-hydroxy propionate high-yielding strain recombinant plasmids and belongs to the production field of biochemical industry. In view of the fact that most conventional yields of 3-hydroxy propionate engineering bacteria are comparatively low, an effective tac promoter applicable to klebsiella pneumonia and puuc aldehyde dehydrogenase capable of converting 3-hydroxy propionaldehyde into 3-hydroxy propionate are discovered to construct and recombine the engineering bacteria. After the sequence of the encoding gene of the enzyme is analyzed, the gene is chemically synthesized; after sequencing confirmation, the promoter and enzyme are overexpressed in klebsiella pneumonia to obtain recombined klebsiella pneumonia pET (ptac-puuc) and the catalytic ability of the enzyme for 3-hydroxy propionaldehyde in vivo and in vitro is measured, wherein in vivo, the enzyme activity of the enzyme for 3-hydroxy propionaldehyde reaches 26.31 U / mg and is 6.9 times of the enzyme activity of the enzyme for original klebsiella pneumonia (3.82 U / mg). 48 hours after optimizing the medium components and IPTG concentration condition, the yield of 3-hydroxy propionate in the engineering bacteria cultivated and reformed in a 5L fermentation tank reaches 73 g / L.

The invention relates to a bacilli, its compound preparation and the preparing method for the compound preparation. Its name is Bacillus subtilis LY-35, which is preserved in the general microbe center of Micro Germ conservation Management committee of China, and its conservation number is:CGMCC N0.1222. The preparation is a mixture of lambda phage of culture of bacilli and staphylococcus, streptococcus, colibacillus, bacillus proteus, pseudomonad, salmonella, pasteurella and klebsiella, can cure and prevent all kinds of illness caused by conditional pathogen,is a micro biological preparation which can be used by being mixed with antibiotics or replace antibiotics without any remainder and pollution, with good curative effect and safety.

The invention relates to a culture method of Klebsiella pneumoniae SnebYK for inducing soybean to generate resistance to atrazine after the treatment of soybean seeds and application thereof, belonging to the technical field of microorganism agricultural pesticide. The invention relates to a bacteria strain SnebYK, which is preserved in China committee for culture preservation of general microorganism centre with the preservation number of CGMCC No.3471 in NOV.24th, 2009. The soybean seeds are treated by a metabolin through culture preparation of liquid fermentation, the soybeans can generate the resistance to atrazine when germinating. The soybean treated by the metabolin of the bacteria can be interbred with the grass family crops including corns and the like, and a uniform atrazine weed killer is adopted to weed out, thereby the difficulty of weeding out the grasses intercropping between the corns and the soybeans is solved, and the Klebsiella pneumoniae has favorable application development prospect.

The invention discloses the application of a protein Com1 in the immunoprotection of coxiella burnetii, which is the application of the protein as shown in SEQ ID NO:1 in the preparation of a Q hot vaccine. The application proves that the recombinant protein Com1 of the coxiella burnetii can serve as an antigen protein, can stimulate an organism to produce an immune response to fight against the attack on virus strains of the coxiella burnetii, and is a protective antigen which has the functions of the Q hot vaccine. The protein Com1 is prepared through a gene engineering method, and the protein Com1 has the advantages of being simple and convenient to operate, needing low cost, and having high yield. As the protein Com1 serves as the Q hot vaccine, the defect of the difficult preparation of vaccines in the prior art is overcome.

The invention relates to a recombinant klebsiella which starts subexpression with kanamycin resistant gene and the application thereof, which belongs to the technical field of gene engineering. The invention provides a recombinant klebsiella to improve the yield of 1,3-propanediol(PDO) and the classification and nomenclature of the recombinant klebsiella is klebsiella (klebsiella sp.) pETPkan-dhaT, the recombinant klebsiella has been preserved at China Center for Type Culture Collection (CCTCC) with an accession number of CCTCC NO:M 208135. The application is to use the gene dhaT from klebsiella 1,3-propanediol redox enzyme to construct a klebsiella expression vector pETPkan-dhaT; to introduce the klebsiella expression vector into the klebsiella to obtain the recombinant klebsiella pETPkan-dhaT, and to enhance the expression of dhaT gene and realize that the accumulation of an intermediate product 3-glyceraldehyde decreases obviously, and the yield of 1,3- PDO increases by 16.9 percent than the counterpart during the course that the recombinant klebsiella has glycerol as a substrate. The method lays a solid foundation for the high yield of 1,3- PDO or the yield of 1,3- PDO klebsiella gene engineering with glucose as a substrate.

The invention provides a Q fever Coxiella burnetii TaqMan fluorescent quantitative PCR (polymerase chain reaction) detection method. A forward primer is 5'-GGGGTAAAGTGATCTACAC-3'; a reverse primer is 5'-CCCATAAACGTCCGATAC-3; and a probe is 5'-TCAGTATGTATCCACCGTAGCCA-3, wherein the 5' end of the probe is marked with a reporter fluorescent dye, and the 3' end is marked with a quenching fluorescent dye. The invention establishes a fluorescent quantitative PCR detection method which can conveniently, quickly and accurately detect Q fever Coxiella burnetii, is beneficial to distinguish between Coxiella burnetii and other pathogens, and is of far reaching importance in Q fever diagnosis of humans and animals.

The invention discloses primers special for detecting coxiella burnetii and provides a loop mediated isothermal amplification primer group for detecting the coxiella burnetii. The loop mediated isothermal amplification primer group comprises a primer 1, a primer 2, a primer 3 and a primer 4. Nucleotide sequences of the primer 1, the primer 2, the primer 3 and the primer 4 are respectively a sequence 1, a sequence 2, a sequence 3 and a sequence 4 in a sequence table in sequence. The primers special for detecting the coxiella burnetii have the advantages of being capable of performing amplification reaction only at constant temperature, free of special devices, high in specificity, rapid, efficient, capable of finishing the amplification reaction within 60 minutes, high in sensitivity, convenient and simple to identify and particularly suitable for rapid detection of clinical specimens.

The invention discloses a PCR primer pair for rodent-borne disease pathogenic microorganisms. The primer pair is used for amplifying specific genes of leptospira interrogans, Rickettsi Mooseri, Orientia tsutsugamushi, anaplasma phagocytophilum, francisella tularensis, Coxiella burnetii and Bartonella. By adopting the PCR primer pair, on the basis of the TSP principle, the method for high-sensitiveand specific independent or combined detection of the seven kinds of pathogene.

The invention discloses a non-diagnostic method for detecting coxiella burnetii with a loop-mediated isothermal amplification (LAMP) technology. An LAMP method has the advantages of easiness in operating, quick reaction, completion within one hour under a constant-temperature condition, low time consumption, one-step completion of reactions and product detection, easiness and convenience in operating and low detection cost. Due to the adoption of the non-diagnostic method, a novel developing direction is provided for the detection of the coxiella burnetii; and the method is expected to become a simple conventional detection means, is particularly suitable for basic level inspection and quarantine institutes, and plays important roles in raising the sanitary level, guaranteeing the human life safety and prompting the development of the sanitary inspection institutions.

The invention provides a monoclonal antibody for quantitative detection of Coxiella burnetii I-phase strains. The monoclonal antibody performs detection in combination with an up-converting phosphor technology; and compared with a traditional Coxiella burnetii detection method, the monoclonal antibody has the advantages that detection is accurate, quantitative and free of background interference,the detection result is stable, and detection is convenient and rapid to perform.

The invention discloses application of recombinant klebsiella peneumoniae in preparation of 1,3-propanediol, particularly relates to application of recombinant klebsiella peneumoniae overexpressing lactobacillus fermenti glycerin channel protein gene glpF in production of 1,3-propanediol and belongs to the field of genetic engineering. By applying a genetic engineering method, an overexpression vector of the lactobacillus fermenti glycerin channel protein gene glpF is transferred to klebsiella peneumoniae, accordingly the glycerin transfer capability of the gene is improved, and the 1,3-propanediol yield of the klebsiella peneumoniae is improved. The recombinant strain is subjected to material supplementation and batched fermentation with a 5L fermentation tank, the yield of 1,3-propanediol is as high as 76g / L. By adopting the application, the glycerin transfer capability of the klebsiella peneumoniae is successively changed, and a new way of thinking is provided for breeding of high-yield 1,3-propanediol strains.

The invention discloses an Umbelopsis sp. strain and a mycelium extract thereof, as well as an application. The Umbelopsis sp. QZ042 was saved in China General Microbiological Culture Collection Center (CGMCC) on August 19, 2013 with the collection number of CGMCC No. 8101. The applicant finds that, the mycelium extract of an Umbelopsis sp. QZ042 culture disclosed by the invention has relatively good anti-bacterial activity against colon bacillus, bacillus cereus, arthrobacter nicotianae, klebsiella, bibrio parahemolyticus medicaments and other bacteria and has obvious antibacterial effect against eurotiales, alternaria, lasiodiplodia, stem canker fungi and other types of fungi through experiments and screening medicaments are provided for researching and developing new anti-bacterial and anti-fungal medicaments.

The invention discloses application of recombinant klebsiella pneumoniae in producing 1,3-propylene glycol and particularly discloses a method for improving the yield of the 1,3-propylene glycol by using mexF gene in heterologously-expressed pseudomonase aeruginosa of the klebsiella pneumoniae and application thereof, and belongs to the technical field of genetic engineering. According to the method disclosed by the invention, by applying a genetic engineering method, plasmid with the mexF gene in the pseudomonas aeruginosa is transferred to the klebsiella pneumoniae for enhancing the transportation efficiency of the klebsiella pneumoniae to short chain alcohol, so that the yield of the 1,3-propylene glycol is improved. The yield of the 1,3-propylene glycol reaches as high as 74g / L by carrying out fed-batch fermentation on arecombinant strain in a 5L fermentation tank. The method has the advantages that the transportation capacity of the klebsiella pneumoniae to the 1,3-propylene glycol is successively improved, provides a novel method for improving the yield of the 1,3-propylene glycol and provides a new idea for breeding high-yielding strains of the 1,3 propylene glycol.

The invention relates to a method for the detection of prior exposure to Coxiella burnetii infection by antibody-based assays using recombinant, immunodominant C. burnetii polypeptides. The invention also relates to the design of biotin or His-tagged C. burnetii proteins useful in the antibody-based assays as standardized antigen reagents.

This invention describes a bacillus subtilis and the process for preparing its compound pharmaceutics. The bacillus subtilis is conserved at China General Microbiological Culture Collection Center of China Committee of Culture Collection for Microorganisms, the name is: Bacillus subtilis LY-35 and the conservation number is: CGMCC No. 1222. The pharmaceutics is a compound of the bacteriophages of such pathogens as bacillus subtilis, Staphylococcus, Streptocoaus, colibacillus, proteus, pseudomonas, salmonella, shigella, pasteurella and klebsiella. The pharmaceutics can prevent and treat the diseases caused by pathogens, and can be a non-polluted, high therapeutic effectiveness and safe substitute for antibiotics.

The invention provides a composite promoter. The composite promoter is formed by arranging 2 to 5 tac promoter core sequences. The invention also provides a carrier with the composite promoter, engineering recombinant bacteria and a preparation method of the engineering recombinant bacteria. The engineering recombinant bacteria with the complex promoter can express aldehyde dehydrogenase efficiently, thereby rapidly catalyzing 3-HPA to produce 3-HP, preventing the accumulation of 3-HPA from weakening the growth and metabolism of the cells on the one hand, and improving the yield of 3-HP and onthe other hand.

The invention relates to a Coxiella burnetii nucleic acid detection kit and particularly relates to a kit for detecting Coxiella burnetii nucleic acid by utilizing a composite probe real-time fluorescent PCR (polymerase chain reaction) technology. The kit carries out qualitative detection on the Coxiella burnetii in a sample, and can be applied to auxiliary diagnosis of Coxiella burnetii infection.

A flavouring composition for use in an oral care product, confectionary product or beverage comprises an antimicrobial key and optionally at least one flavouring ingredient of current use, wherein the antimicrobial key comprises 3,4-dimethylphenol together with one or more antimicrobial flavour ingredients each having a minimum inhibitory concentration of 1000 parts per million or less, against two or more strains selected from Fusobacterium Nucleatum, Fusobacterium sp., Porphyromonas Gingivalis, Prevotella Intermedia, Klebsiella Pneumoniae, Veillonella Alcalescens, Bacteroides Melaninogenicus / forsythus, Selenomonas Sputagena, Porphyromonas Endodontalis, Prevotella Melaninogenica and Streptococcus Mutans.

The invention discloses a CRISPR-Cas13a (Clustered Regularly Interspaced Short Palindromic Repeats Cas13a) system for detecting coxiella burnetii nucleic acid. The invention provides crRNA. The crRNAis shown as a sequence 1 in a sequence table. The invention further provides a nucleic acid molecule composition. The nucleic acid molecule composition is composed of a primer Coxiella-F, a primer Coxiella-R and the crRNA shown in the specification. The primer Coxiella-R is a single-stranded DNA (deoxyribonucleic acid) molecule shown as a sequence 7 in the sequence table. The invention also discloses an application of any one of the crRNA or the nucleic acid molecule composition in detection of coxiella burnetii nucleic acid. The invention discloses a CRISPR-Cas13a detection system for detecting coxiella burnetii nucleic acid. The CRISPR-Cas13a detection system achieves higher-sensitivity, high-specificity, rapid and accurate detection on a trace coxiella burnetii nucleic acid sample. Theinvention has important application and popularization value for prevention and control of Coxiella burnetii infection.

Vaccines and methods are for immunization against Coxiella burnetii infections and Q fever. The vaccine can be for protection against a Coxiella burnetii infection, or treating or preventing at least one symptom of Q fever. The vaccine includes a delipidated C. burnetii polysaccharide derived from a cell wall lipopolysaccharide of C. burnetii. The vaccine can also be a conjugate vaccine for protection against a Coxiella burnetii infection, or treating or preventing at least one symptom of Q fever. The conjugate vaccine includes a delipidated C. burnetii polysaccharide derived from a cell wall lipopolysaccharide of C. burnetii linked to an immunogenic carrier.

The invention provides primers and a method for Q fever Coxiella burnetii SYBR GreenI fluorescent quantitative PCR (polymerase chain reaction) detection. A forward primer is 5'-TGCTTCAGTATGTATCCAC-3'; and a reverse primer is 5'-GTTACGATCATTCTTGTTAC-3'. The invention establishes a fluorescent quantitative PCR detection method which can conveniently, quickly and accurately detect Q fever Coxiella burnetii, is beneficial to distinguish between Coxiella burnetii and other pathogens, and is of far reaching importance in Q fever diagnosis of humans and animals.

The invention discloses an Umbelopsis sp. strain and a mycelium extract thereof, as well as an application. The Umbelopsis sp. QZ042 was saved in China General Microbiological Culture Collection Center (CGMCC) on August 19, 2013 with the collection number of CGMCC No. 8101. The applicant finds that, the mycelium extract of an Umbelopsis sp. QZ042 culture disclosed by the invention has relatively good anti-bacterial activity against colon bacillus, bacillus cereus, arthrobacter nicotianae, klebsiella, bibrio parahemolyticus medicaments and other bacteria and has obvious antibacterial effect against eurotiales, alternaria, lasiodiplodia, stem canker fungi and other types of fungi through experiments and screening medicaments are provided for researching and developing new anti-bacterial and anti-fungal medicaments.