110 results about "Klebsiella pneumonia" patented technology

A method for treating a Klebsiella pneumonia infection in a mammal in need thereof is disclosed, the method comprising administering to the mammal an effective amount of an antibacterial aminoglycoside compound.

The invention discloses a primer and a probe for quantitative determination of klebsiella pneumonia, and an application of the primer and the probe. Nucleotide sequences of the primer are a sequence 3 and a sequence 4 in a sequence table; a nucleotide sequence of the probe is a sequence 5 in the sequence table. The primer and the probe have the beneficial effects that the specific primer and probe sequences of the klebsiella pneumonia disclosed by the invention can be applied to qualitative and quantitative detection of the klebsiella pneumonia, the target of accurately and quantitatively determining the deoxyribonucleic acid (DNA) content of the klebsiella pneumonia in a specimen to be detected can be achieved by extracting the DNA in the sample to be detected and combining with a real-time fluorescence quantification polymerase chain reaction (PCR) detection technology, and the primer and the probe can be applied to food detection, scientific research and clinical diagnosis and used for carrying out qualitative and quantitative analysis on the klebsiella pneumonia DNA in samples such as a food sample, a suffer throat swab, nasopharyngeal secretion, people sputum specimens and blood samples. Thus, the primer and the probe have an important significance on judgment of klebsiella pneumonia infection, evaluation of treatment effectiveness and dynamic observation of the state of an illness, and simultaneously play an important role in the field of detection of clinical medicine.

The invention discloses a high yield microbe of 1,3-propylene glycol, and concretely discloses a separated microbe, a method and a system for producing PDO, wherein the separated microbe is Klebsiella pneumonia, and is preserved at the common microorganisms center of the CCCCM on 11th, July, 2013, a preservation number is CGMCC NO.M 7824, and the preservation name is ACR30. The Klebsiella pneumonia has thin capsule, and the PDO produced by using glycerin fermentation has the advantages of good performance and high production strength.

The invention discloses recombination klebsiella pneumonia capable of co-producing 3-HP and P3HP, and a preparation method and application thereof. The recombination bacterium is obtained by introducing glycerol dehydratase gene, glycerol dehydratase reactivation enzyme gene, aldehyde dehydrogenase gene, propionyl coenzyme A synthetase gene and polyhydroxyalkanoate synthetase gene into a host recombination klebsiella pneumonia in which 1, 3-propylene glycol oxidoreductase gene and aldehyde reductase / alcohol dehydrogenase gene are knocked out. According to the technical scheme, the production cost of 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) is reduced, and 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) can be synthesized at the same time by taking a same thallus as the host.

The invention relates to a compound microbial preparation capable promoting the root development of crops and particularly relates to compound microbial preparation capable of promoting the root development of wheat. The compound microbial preparation contains the following components in parts by weight: 1-3 parts of bacillus subtilis, 1-5 parts of rhodopseudomonas palustris, 1-3 parts of rhizobium, 1-2 parts of paenibacillus kribbensis, 3-5 parts of bacillus amyloliquefaciens, 1-3 parts of klebsiella pneumonia, 3-10 parts of ammonium sulfate, 1-8 parts of potassium sulfate, 1.5-2.8 parts of fulvic acid, 0.1-3 parts of saccharose, 5-12 parts of diammonium phosphate, 0.1-0.6 part of boric acid, 0.01-0.8 part of sodium chloride, 1.5-3.8 parts of chitosan, 5-20 parts of plant ash, 0.1-3 parts of starch powder, 0.1-3 parts of calcium carbonate, 0.01-0.6 part of lauryl sodium sulfate and 5-20 parts of vermiculite. According to the compound microbial preparation, the normal development of roots is promoted, the lodging resistance of the wheat is enhanced, the yield increase of the wheat is promoted, and the yield is increased by 8%-15%.

The invention relates to a multiplex PCR-based synchronous and rapid method for detecting 13 pathogenic microorganisms in water, which comprises the steps of using multiplex PCR to simultaneously amplify gene-specific fragments of the 13 pathogenic microorganisms including escherichia coli, enterohaemorragic escherichia coli o157:h7, legionella pneumophila, salmonella enteritidis, shigella dysenteriae, staphyloccocus aureus, listeria monoeytogenes, helicobacter pylori, mycobacterium tuberculosis, klebsiella pneumonia, vibrio cholera, bacillus anthracis and yersinia pestis, and detecting PCR amplified products through agarose gel electrophoresis, thereby achieving synchronous and rapid detection for the 13 pathogenic microorganisms.

The invention discloses pseudomonas aeruginosa bacteriophage endolysins, and a coding gene and application thereof. The invention provides a protein which is as shown in (a) or (b) as below: (a) the protein which is as shown in a sequence 1 in a sequence table and consists of an amino acid sequence; (b) the protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence which is as shown in the sequence 1 in the sequence table, has a function of cracking gram-negative bacteria and is deviated from the sequence 1 in the sequencetable. The protein provided by the invention can crack a plurality of gram-negative bacteria, such as Escherichia coli, Klebsiella pneumonia, pseudomonas aeruginosa, Burkholderia cepacia and Salmonella typhimurium. The protein and a surfactant EDTA (Ethylene Diamine Tetraacetic Acid) act synergistically to achieve a stronger inhibiting effect on the gram-negative bacteria. A foundation is laid for researching and developing a new antibacterial preparation.

The invention discloses a PCR (Polymerase Chain Reaction) amplification primer for quickly detecting klebsiella pneumonia and application thereof, and belongs to the field of animal bacteriology and molecular biology. The PCR amplification primer is prepared from an upstream primer having a nucleotide sequence as shown in SEQ ID NO:1 and a downstream primer having a nucleotide sequence as shown inSEQ ID NO:2; the PCR amplification primer is used for preparing a PCR amplification kit for detecting the klebsiella pneumonia. A PCR detecting method provided by the PCR amplification kit has high specificity and sensitivity, good repeatability and high credibility and is capable of specifically detecting the klebsiella pneumonia and quickly and accurately obtaining a detecting result; meanwhile, the PCR amplification kit is low in cost and simple in operation, is suitable for being used at the primary level and can be used as a quick, accurate and simple detecting tool for quick laboratoryidentification of the klebsiella pneumonia and large-scale epidemiological investigation.

The invention discloses a longifolene derivative (2, 2, 8, 8-tetramethyl-4-atrane [5. 4. 0. 11, 9] dodecane-6-alkene obtained by rearrangement and reduction reaction and by taking isolongifolene ketoxime as a raw material. Besides, bacteria such as staphylococcus aureus, Klebsiella pneumonia, Escherichia coli, bacillus proteus vulgaris and pseudomonas aeruginosa can be inhibited by the longifolene derivative, and particularly, the longifolene derivative has a superior insecticidal effect on pests such as aphids and rice planthopper.

The invention discloses indole compounds represented by the general formula defined in the specification and salts and preparation method thereof, corresponding medical uses of the compounds and the salts thereof, and drugs and disinfectants containing the compounds and the salts thereof. A lot of experiments prove that the compounds and the salts thereof have good bacteriostatic and bactericidal activity on MRSA (drug-resistant staphylococcus aureus), MDR K.pneumonia (drug-resistant klebsiella pneumonia), and MDR A.baumanii (drug-resistant acinetobacter baumannii). In the formula, X is methylene, carbonyl or sulfonyl; R1 is fluorine, chlorine, bromine, methoxy acyl, nitro or hydrogen; R2 is methoxyl acyl, (p-methoxyl acyl)phenyl, (p-trifluoro oxy)phenyl, (p-trifluoro)phenyl, (m-methoxy acyl)phenyl, (m-trifluoro oxy)phenyl, (p-fluoro m-chloro)phenyl or hydrogen; and HA is inorganic acid or a part of organic acid with strong acidity, preferably hydrochloric acid or hydrobromic acid.

The invention relates to a real-time fluorescent PCR kit, and especially relates to a kit used for detecting klebsiella pneumonia (KP) by using a real-time fluorescent PCR technology. With the kit, KP in samples can be detected. The kit can be widely applied in auxiliary diagnosis of KP infection.

The invention belongs to the field of quality control of microbiological detection, and particularly relates to a sample for verifying capacity of escherichia coli in a medicine and a preparation method thereof. The sample for verifying the capacity of the escherichia coli in the medicine comprises a target flora and a background flora, wherein the target flora is escherichia coli; the background flora consists of klebsiella pneumonia, staphylococcus aureus and bacillus cereus. The uniformity and the stability of the sample meet capacity verifying requirements, so that the stability of a test sample can be ensured to the maximum extent, and even during transportation and storage, change of the escherichia coli content does not affect the total amount of the escherichia coli in the statistical sense; the preparation method of the sample is simple in process and high in successful rate.

The invention discloses an LAMP primer combination for detecting 8 environmental pathogens of dairy cow mastitis and application thereof. The primer combination provided by the invention is composed of 48 primers shown in sequence 1 to sequence 48. The primer combination provided by the invention can be used for detecting whether a to-be-detected bacterium is Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus dysgalactiae, Streptococcus uberis, Salmonella typhimurium, Proteus mirabilis or Candida albicans, and can be used for detecting whether a to-be-detected sample contains Escherichia coli and / or Klebsiella pneumonia and / or Pseudomonas aeruginosa and / or Streptococcus dysgalactiae and / or Streptococcus uberis and / or Salmonella typhimurium and / or Proteus mirabilis and / or Candida albicans. The primer combination provided by the invention is used for identifying and detecting the 8 environmental pathogens of dairy cow mastitis, has high specificity and high sensitivity, and can realize simple, rapid and accurate detection, thus having great popularization value.

The invention discloses LAMP (loop-mediated isothermal amplification) primers, a kit and a method for detecting murine klebsiella pneumoniae. A visual detection method of murine klebsiella pneumonia which is suitable for clinical sample detection and has strong specificity and high sensitivity is established by designing the LAMP primers by virtue of a klebsiella pneumoniae tyrB gene sequence. Whether klebsiella pneumoniae exists or not can be detected quickly and accurately by observing whether green fluorescence exists or not, and the lowest detectable limit is 5.6 copy number / reaction. According to identification of a specific sequence region of a target sequence by four primers designed and screened, high specificity of LAMP diffusion is guaranteed, so that the LAMP primers are suitable for field application at basic level.

The invention relates to a method for treating chemical industrial sewage with klebsiella pneumonia. The method comprises the following steps: arranging a bacterial carrier in a bioreactor so that klebsiella pneumoniae is adsorbed on the bacterial carrier; adding the chemical industrial sewage inside a bioreactor so that the concentration of klebsiella pneumoniae is 10.2 billion-10.8 billion per milliliter; and treating the chemical industrial sewage inside the bioreactor for 0.5-72 hours. According to the method of treating chemical industrial sewage with klebsiella pneumoniae, the organic pollutants in the chemical industrial sewage are decomposed with klebsiella pneumoniae, the chemical industrial sewage is efficiently treated and the treated drainage water can reach the industrial water standard.

The invention belongs to the field of quality control of microbiological detection, and particularly relates to a sample for verifying capacity of staphylococcus aureus in a medicine and a preparation method thereof. The sample for verifying the capacity of the escherichia coli in the medicine comprises a target flora and a background flora, wherein the target flora is staphylococcus aureus; the background flora consists of escherichia coli, klebsiella pneumonia and bacillus cereus. The uniformity and the stability of the sample meet capacity verifying requirements, so that the stability of a test sample can be ensured to the maximum extent, and the total amount of the escherichia coli is not affected in the statistical sense; the preparation method of the sample is simple in process and high in successful rate.

The invention discloses a medical PP inorganic antibacterial composite and a preparation method thereof. The medical PP inorganic antibacterial composite is prepared by use of the steps of weighing PP, divinyl benzene, a nano-zirconium phosphate silver-loaded antibacterial agent, an AC foaming agent, calcium stearate, PE, an antioxidant, paraffin, dicumyl peroxide, titanium dioxide, a surface treating agent, thiodipropionic acid dilauryl ester, azodicarbonamide, sodium benzoate and a titanate coupling agent in parts by weight, mixing all the components evenly and then extruding and pelletizing. The relative density of the obtained composite is 0.05-0.45 and the tensile strength of the composite is 2.5-4.5MPa; besides, the composite has the antibacterial rate of 99.5-99.9% to escherichia coli, 99.5-99.9% to staphylococcus aureus and 99-99.5% to pseudomonas aeruginosa, the bending strength of 11-15MPa, the degree of crosslinking of 45-55%, the impact strength of 3-5kJ / m<2>, and the bacteriostasis rate of 92-96% to salmonella typhimurium and Klebsiella pneumonia.

The invention relates to a 3-aryl-4-(2-glycosyl / amino ethyoxyl)-2(5H)-furanone compound. The 3-aryl-4-(2-glycosyl / amino ethyoxyl)-2(5H)-furanone compound has a structural general formula shown as the specifications. The 3-aryl-4-(2-glycosyl / amino ethyoxyl)-2(5H)-furanone compound has good inhibition effect on staphylococcus epidermidis, Klebsiella pneumonia, novel cryptococcus gattii and the likeand can be used for preparing anti-infective medicaments for treating pneumonia, wound suppuration and the like. The invention discloses a preparation method of the 3-aryl-4-(2-glycosyl / amino ethyoxyl)-2(5H)-furanone compound.

The invention discloses a development culture medium for separating and identifying pathogens in a urogenital tract, which at least comprises three development substrates, namely a hexosaminidase substrate, a beta-D-galactosidase substrate, a beta-D-glucuroide substrate. The development culture medium added with the three development substrates is prepared into a microbial culture medium agar plate, a sample or a bacterial colony subjected to separate culture is inoculated into the development plate and is incubated, and a result can be directly observed. In the development culture medium, various pathogens including bacteria and fungi in a genital tract can be simultaneously cultured and identified on the same development plate, namely bacterial pathogens such as Escherichia coli, Klebsiella pneumonia, enterococcus, pseudomonas aeruginosa, proteus mirabilis, staphylococcus aureus and the like and fungal pathogens such as candida albicans, candida tropicalis and the like can be simultaneously identified, and judgment can be performed through visual inspection; and the development culture medium is quick and convenient to use, and easy to operate.

The invention provides carborane derivatives and application thereof. The structural formulas of three relative novel carborane derivatives are shown as (1), (2) and (3), and antibacterial medicines used as active ingredients of a medicine have the effects of resisting clinically common pathogenic bacteria (staphylococcus aureus, acinetobacter baumannii, klebsiella pneumonia, and proteus mirabilis), and also have the same-degree effect of resisting multidrug-resistant staphylococcus aureus strains and can inhibit multidrug resistance of the multidrug-resistant staphylococcus aureus strains, wherein a compound (2) can react with medicine-resistant staphylococcus aureus extracellular proteins, so adhesion of bacteria on the surface of an organism is reduced, and the formation of a medicine-resistant strain biological film is inhibited. The toxicity and invasiveness of staphylococcus aureus are reduced, treatment efficiency is improved, an adverse effect caused by using a large amount ofantibiotics is avoided, and a novel compound is provided for finding a novel medicine for resisting medicine resistance bacteria.

The invention provides a textile material and a preparation method thereof. The textile material is mainly prepared from the following raw materials in weight percent by blending: 5%-10% of wool fibers, 56%-70% of natural ocean high-polymer chitosan fibers, 10%-20% of milk protein fibers and 12%-15% of chinlon. According to the textile material, the antibacterial rate on staphylococcus aureus is 99.7%-99.99% and the antibacterial rate on Klebsiella pneumonia is 99%-99.99%; the number of times of washing is 80-90 and the burst strength is 470N-580N, and the washing color fastness reaches grade 5; and the textile material has good hand feeling, is comfortable to wear and is suitable for producing textiles including shirts, underwear, nightclothes, hats, gloves, sports wear, bedding articles and the like.

The invention discloses a post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and an application thereof. The probe comprises a first group of probes to a seventeenth group of probes, and the first group of probes to the seventeenth group of probes are probes for detecting Neisseria meningitides, Bacillus pyocyaneus, Salmonella, Stenotrophomonas maltophilia, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Serratia marcescens, Acinetobacter lwoffii, Corynebacteria diphtheria, Streptococcus mitis, Acinetobacter baumannii, Streptococcus pneumonia, Propionibacterium acnes, Listeria monocytogenes, Enterobacter cloacae and Haemophilus influenza sequentially. The probe is developed through an MLPA technology, can rapidly diagnose post-neurosurgical intracranial bacterial infection 17 common pathogenic bacteria once, provides possibility for rapid diagnosis of pathogenic bacteria of like patients, and is helpful for reducing the fatality and the disability rate of like infection.

The invention discloses a gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in a water body. The method is characterized in that specific gene segments of the pathogenic microorganisms are amplified simultaneously by adopting multiple polymerase chain reactions (PCR), and then, a detection result is obtained through gene chip hybridization and chip scanning image analysis. The detected thirteen pathogenic microorganisms comprise escherichia coli, Enterohemorrhagic Escherichia coli O157:H7, legionella pneumophila, salmonella enteritidis, Shigella, staphylococcus aureus, Listeria monocytogenes, Helicobacter Pylori, mycobacterium tuberculosis, Klebsiella pneumonia, vibrio cholera, Bacillus anthracis and Yersinia pestis.

The invention discloses a kit for detecting klebsiella pneumonia by using a nucleic acid constant-temperature amplification technology and an amplification method thereof. Sequences of six primer pairs, namely, 5'-TACGCCCCGGTCTGAC, 5'-GCGCTTTCACCCCCAAC, 5'-GTCCCTTTTGCCCGAGGTCGACGGCACGGCCATT, 5'-CGACGGCACGGCCATTTGTCCCTTTTGCCCGAGG, 5'-TGGCAACGACTGGTCTCGCC and 5'-TCGCGTAGGGGGAGGCTGTT, are designed. Aiming at the klebsiella pneumonia, the invention overcomes the defect in the prior art and provides a quick and low-cost detection method based on the nucleic acid constant-temperature amplification technology.

The invention relates to a mixed microbial preparation for recovering soil polluted by composite pollutant oil in a biology manner and a manufacture method of the mixed microbial preparation. The mixed microbial preparation is made by mixing six bacterial strains including acinetobacter calcoaceticus, klebsiella pneumonia, enterobacter cancerogenus, pseudomonas stutzeri, rhodococcus erythropolis, and pseudomonas putida with one of rhodococcus, acinetobacter, gordonia, sphingobacterium, novosphingobium capsulatum, acinetobacter, and xanthomonas at a ratio of 1:1:2:2:1:1:1:1 and is used for recovering oil-polluted soil.

The invention discloses a construction method of 3-hydroxy propionate high-yielding strain recombinant plasmids and belongs to the production field of biochemical industry. In view of the fact that most conventional yields of 3-hydroxy propionate engineering bacteria are comparatively low, an effective tac promoter applicable to klebsiella pneumonia and puuc aldehyde dehydrogenase capable of converting 3-hydroxy propionaldehyde into 3-hydroxy propionate are discovered to construct and recombine the engineering bacteria. After the sequence of the encoding gene of the enzyme is analyzed, the gene is chemically synthesized; after sequencing confirmation, the promoter and enzyme are overexpressed in klebsiella pneumonia to obtain recombined klebsiella pneumonia pET (ptac-puuc) and the catalytic ability of the enzyme for 3-hydroxy propionaldehyde in vivo and in vitro is measured, wherein in vivo, the enzyme activity of the enzyme for 3-hydroxy propionaldehyde reaches 26.31 U / mg and is 6.9 times of the enzyme activity of the enzyme for original klebsiella pneumonia (3.82 U / mg). 48 hours after optimizing the medium components and IPTG concentration condition, the yield of 3-hydroxy propionate in the engineering bacteria cultivated and reformed in a 5L fermentation tank reaches 73 g / L.

The invention discloses a novel drug resistant gene of Klebsiella pneumonia. The novel drug resistant gene of the Klebsiella pneumonia is obtained by extracting the Klebsiella pneumonia which is drug resistant against imipenem and ertapenem. The nucleotide sequence of the novel drug resistant gene of the Klebsiella pneumonia is SEQ ID NO. 1 and the amino acid sequence is SEQ ID NO. 2. Functions of the novel gene are researched through a plasmid transduction experiment and a prokaryotic expression experiment, and a result shows that the novel gene is drug-resistant against a plurality of drugs. The novel drug resistant gene of the pneumonia Klebsiella has a great significance on reasonable use of antibiotics, reduction of carbapenems drug-resistant bacteria produced during severe infection, and research and development of new drugs for inhibiting drug-resistance performance of bacteria.

The invention relates to medical in-vitro diagnostic techniques, in particular to a reverse dot-blot hybridization gene chip. A plurality of microarrays in different areas are distributed on a nylon membrane, and a common specific probe for postoperative intracranial infection pathogenic bacteria is fixed in each microarray area. The reverse dot-blot hybridization gene chip can be used for identifying enterococcocci (enterococcus faecium and enterococcus faecalis), staphylococcus aureus, coagulase-negative staphylococci, escherichia coli, Klebsiella pneumonia, pseudomonas aeruginosa, acinetobacter baumannii and enterobacter cloacae. The reverse dot-blot hybridization gene chip further comprises a universal Gram-negative bacterium probe and a universal Gram-positive bacterium probe. The chip can be used for simultaneously detecting whether various pathogenic bacteria exist or not, has the advantages of rapidity and accuracy in diagnosis, high specificity and heavy information, and is of great help to clinical diagnosis and epidemic disease screening.

The invention discloses an NAD<+>-independent aldehyde oxidase catalyzing production of 3-hydroxypropionic acid from 3-hydroxypropionaldehyde, and its application, belongs to the biochemical production field, and relates to the NAD<+>-independent aldehyde oxidase enabling Klebsiella pneumonia to maintain the intracellular coenzyme balance in the catalysis process of the production of 3-hydroxypropionic acid from 3-hydroxypropionaldehyde. The sequence of the aldehyde oxidase is represented by SEQ ID NO:1 in a sequence table. Mass screening results show that the aldehyde oxidase in Pseudomonas sp. is independent of NAD<+>, and can catalyze the oxidation of aldehydes to corresponding carboxylic acids. The overexpression of the aldehyde oxidase in the Klebsiella pneumonia is carried out, and the in-vivo and in-vitro 3-hydroxypropionaldehyde catalysis ability is determined. The above enzyme has good 3-hydroxypropionic acid production ability.

The invention discloses a phage depolymerizing enzyme Depo43 based on a klebsiella pneumoniae phage P560 and an application of the phage depolymerizing enzyme Depo43. The phage P560 disclosed by the invention is preserved in China Center for Type Culture Collection on October 26, 2020, the preservation name is Klebsiella pneumonia phage P560, and the preservation number is CCTCC NO: M 2020643. A 43rd open reading frame of the phage P560 is subjected to prokaryotic expression and purification to obtain the phage depolymerizing enzyme Depo43, and the depolymerizing enzyme not only can remove capsular polysaccharides formed by K47 and K64 klebsiella pneumoniae, but also has an efficient inhibition effect on the formation of biofilms. The epolymerizing enzyme can be widely applied to preparation of antibiotic substitute preparations and medical instrument disinfectants, and has a remarkable clinical effect.