Gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in water body

Abstract

The invention discloses a gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in a water body. The method is characterized in that specific gene segments of the pathogenic microorganisms are amplified simultaneously by adopting multiple polymerase chain reactions (PCR), and then, a detection result is obtained through gene chip hybridization and chip scanning image analysis. The detected thirteen pathogenic microorganisms comprise escherichia coli, Enterohemorrhagic Escherichia coli O157:H7, legionella pneumophila, salmonella enteritidis, Shigella, staphylococcus aureus, Listeria monocytogenes, Helicobacter Pylori, mycobacterium tuberculosis, Klebsiella pneumonia, vibrio cholera, Bacillus anthracis and Yersinia pestis.

Description

Technical field [0001] The invention is environmental science and engineering technology. Background technique [0002] At this stage, the methods commonly used at home and abroad to detect and identify pathogenic microorganisms mainly include traditional biochemical appraisal, immunology detection technology, PCR and other simple molecular biology and immunology methods. [0003] The traditional biochemical appraisal method (such as separation training, biochemical appraisal, etc.) is the most widely used. It is the national standard testing procedure such as "Food Sanitary Microbiology (GB / T 4789-2003)".The qualitative and quantitative results, but most of them need to go through 4 steps: pre -bacterial increase, bacterial increase, selective tablet separation, biochemical test and serum scientific types of identification. The entire process usually takes 3 to 7 days.The detected bacterial proliferation is a visible colonial. In addition, the detection of qualifications, bacter...

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Problems solved by technology

Although PCR is a specific, sensitive, simple and rapid detection technology, but this method requires a long time for sample purification process, and once there is a very small amount of exogenous DNA contamination, false positive results may occur; multiple pairs of primers simultaneously Amplification, improper control of various experimental conditions can easily lead to amplification failure or non-specific products; improper primer design and target sequence selection may reduce its sensitivity and specificity; at the same time, generally only one or few microbes

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