42 results about "Enterohemorrhagic e coli" patented technology

The invention discloses a detection kit and a detection method for 9 species of pathogenic organisms in marine products. The kit comprises Taq DNA polymerase with a concentration of 5U / muL and a PCR reaction solution, wherein the PCR reaction solution contains 10 millimols of Tris.HCl, 50 millimols of KCl, 25 millimols of MgCl2, 2.5 millimols of dNTP and primer pairs of the 9 species of pathogenic organisms. The kit and the method can synchronously detect the 9 species of pathogenic organisms and can detect salmonella with a concentration of 11 CFU / mL, staphylococcus aurei with a concentration of 2,000 CFU / mL, monocytogenes with a concentration of 120 CFU / mL, enterohemorrhagic Escherichia coli O157:H76 with a concentration of CFU / mL, comma bacillus with a concentration of 220 CFU / mL, Vibrio parahaemolyticus with a concentration of 60 CFU / mL, Vibrio vulnficus with a concentration of 9 CFU / mL and Vibrio alginolyicus with a concentration of 230 CFU / mL. The detection method short in detection time and simple and quick in operation, and can save a large amount of labor and financial resources and meet requirements for quick detection.

The present invention relates to prevention and control of highly pathogenic Enterohemorrhagic Escherichia coli O157 in a sewage treatment system, and belongs to the field of bioengineering. The invention provides a strain of a novel phage for lysis of Enterohemorrhagic Escherichia coli O157 in a sewage treatment system, wherein the phage is named PE-3 (gene sequence SEQ ID NO.1, GenBank Access no.KJ748011), and belongs to T7 Podoviridae, the head is positive hexahedron and stereoscopic symmetry and the diameter is about 50 nm, the tail is short and the length and the width are about 12 nm, and the total length of the genome is 39093 bp. According to the present invention, the phage has the strong lysis effect on Enterohemorrhagic Escherichia coli O157, can effectively prevent and control the production and the transmission of Enterohemorrhagic Escherichia coli O157, has high temperature tolerance and wide acid-alkali tolerance range, can be easily and industrially produced, and can be adopted as the safe, reliable and efficient biological disinfectant for rapid and efficient prevention and control of sewage discharge or for reuse of the pollution caused by Enterohemorrhagic Escherichia coli O157.

Compositions and methods for stimulating an immune response against a secreted enterohemorragic Escherichia coli (EHEC) antigen are disclosed. The compositions comprise EHEC cell culture supernatants.

The invention discloses a poly valency fuse type enterorrhagia property bacillus coli O157:H7 gene engineering vaccine, which comprises the following steps: adopting enterorrhagia property bacillus coli O157:H7 Vi antigen Shiga's toxin II; combining subunit, compact sticking element and III type secretory protein A; constructing fuse engineering bacteria through gene retooling method; proceeding high density ferment; proceeding a series of purity course; getting fuse protein molecule vaccine with high purity. This invention possesses simple craft, good repeatability and higher protein purity.

The invention belongs to the pharmaceutical biotechnology field, and relates to enterohemorrhagic E.coli O157:H7 Shiga toxin IIB epitope peptide and the application thereof. Four preferred polypeptides are derived from B-cell epitope of the subunit (Stx2A1) of EHEC O157:H7 Shiga toxin IIB epitope peptide. The product can be applied for preparing pharmaceuticals for the diagnosis and treatment of EHEC O157 infection and complications thereof.

The present invention provides methods and compositions for production of gram-negative bacterial mutants that are defective in intestinal colonization capacity and sensitive to infection by bacteriophage P1. Thus the present invention provides immunogenic compositions for the prevention or attenuation of food- and water-borne illnesses associated with ingestion of bacteria such as enterohemorrhagic Escherichia coli.

The present invention relates to a new isolated polypeptide nominated microcin S, isolated nucleic acid molecules encoding the microcin S polypeptide and primers and probes hybridizing to the nucleic acid molecules. The invention also relates to plasmids and cells comprising the nucleic acid molecules, an antibody binding to the polypeptide, compositions as well as methods for producing and using the polypeptides. The present invention further relates to medical uses for treating or preventing microbial infections, functional gastrointestinal disorders or treating a tumor. The invention further relates to a method for preserving food and a method for coating dressing material.

A set of oligonucleotide probe for detecting the enterohemorrhagic E. coli O157:H7 and cholera vibrio O139 is designed on the Shiga-like toxin generating gene stx1 or stx2 and the beta-glucuronidase gene uidA of said enterohemorrhagic E coli O157:H7 and the enterotoxin A subset (ctxA), toxicity coordinate pilus A subset (tcpA) and glucosyltransferase (LPS gt) genes of cholera vibrio O139, and has high sensibility and specificity. It can be used to detect the enterohemorrhagic E. coli O157:H7, cholera vibrio O139 and other pathogenic genes from the specimen.

The invention relates to a spice plant extract, and particularly relates to an ethanolic extract of cinnamons and an application thereof in preparing food preservatives; a natural food preservative is prepared by combining the ethanolic extract of cinnamons with a food additive; and the ethanolic extract of cinnamons provided by the invention has a good sterilization effect on various typical food-borne pathogenic bacteria, especially has a significant sterilization effect on the pathogenic bacteria O157: H7 of EHEC (enterohaemorrhagic escherichia coli, EHECO157: H7) food poisoning continuously occurring around the world in recent years, and has no side effects on food, therefore, the ethanolic extract of cinnamons is a natural food preservative which is safe and has no side effects; the ethanolic extract of cinnamons is widely applied to the decomposition prevention of products such as fresh vegetables and fruits and beef hamburgers and the like; and in the aspect of freshness retaining, the ethanolic extract of cinnamons not only can effectively prolong the shelf life of food, but also can effectively prevent the occurrence of bacterial food poisoning and effectively ensure the safety of food, therefore, the ethanolic extract of cinnamons plays an important role in the field of food health and safety.

The invention discloses a method for detecting live enterohemorrhagic escherichia coli O157: H7. The method solves the problem that the existing real-time fluorescence PCR technology cannot effectively distinguish live or dead enterohemorrhagic escherichia coli thereby leading to overestimation of a pathogenic bacterium risk. The method comprises the following steps of pre-treating a liquid sample needing to be detected, adding propidium monoazide (PMA) into the pre-treated liquid sample, controlling a PMA final concentration to 15-20 micrograms per milliliter, carrying out incubation in the dark at a normal temperature, carrying out halogen tungsten lamp radiation treatment, extracting a genome DNA of the treated sample, utilizing the extracted sample genome DNA as a template, carrying out real-time fluorescence PCR quantitative detection of object enterohemorrhagic escherichia coli in the sample, wherein primers and fluorescent label probes used by the real-time fluorescence PCR quantitative detection are shown in the formula of SEQ ID NO.1. The method has short operation time of about 1.5h and can be widely used in the field of food-borne pathogenic bacterium detection.

The invention discloses a gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in a water body. The method is characterized in that specific gene segments of the pathogenic microorganisms are amplified simultaneously by adopting multiple polymerase chain reactions (PCR), and then, a detection result is obtained through gene chip hybridization and chip scanning image analysis. The detected thirteen pathogenic microorganisms comprise escherichia coli, Enterohemorrhagic Escherichia coli O157:H7, legionella pneumophila, salmonella enteritidis, Shigella, staphylococcus aureus, Listeria monocytogenes, Helicobacter Pylori, mycobacterium tuberculosis, Klebsiella pneumonia, vibrio cholera, Bacillus anthracis and Yersinia pestis.

The present invention provides a detection kit for E.coli stx2 gene. The E.coli stx2 gene detection kit includes two pairs of primers designed based on the loop-mediated constant temperature amplification technology with the stx2 gene as the target gene. : inner primer FIP / BIP and outer primer F3 / B3. The enterohaemorrhagic Escherichia coli stx2 gene detection kit of the present invention has more comprehensive detection effect and low missed detection rate.

The present invention belongs to the field of biomedicine preparing technology, and is especially enterohemorrhagic E. coli (EHEC) O157 shiga-like toxin II resisting subunit Stx2A monoclonal antibody 5F3 light and heavy chain variable region gene and the gene coded polypeptide and their application in preparing medicine for diagnosing and treating EHEC O157 infection and its complication. The present inventor adopts common facultative primer to culture one hybridoma cell strain of Stx2A specific monoclonal antibody 5F3 and clones its corresponding light and heavy chain variable region gene. Based on the light and heavy chain variable region gene, several small molecular gene engineering antibodies, such as ScFv antibody, chimeric antibody, antibody fusing protein, etc are constituted and expressed. The present invention provides new way for diagnosing and treating EHEC O157 infection and its complication.

This invention relates to a multiple PRC quick test reagent box for pathogens often seen in animal and bird meats and aquatic products including 10xPCR buffer solution, MgCl2, dNTP, primers and DNA poly-enzymes. The tet method includes: picking up a due test sample template, adding 10xPCR buffer solution, MgCl 2, dNTP, primers, DNA poly-enzymes, the due test sample template and ddH20 water to be mixed and enlonged on a PCR instrument, electrophoresis and expansion are made to the product in an electrophoresis device to register the result to be analyzed and judged.

The invention discloses a enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 recombinant protein, including 1) enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 and expression vector fragment; or 2) enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 protein carboxyl terminal acquires protein and expression vector fragment with similar function by losing and adding one or more amino terminals. The invention also discloses preparation of aforementioned enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 recombinant protein, application thereof in preparation of test reagent box and preparation of subunit vaccine for prevention and cure infection of EHEC O157:H7.

The invention discloses preparation and applications of a nucleic acid lateral flow test strip kit for detecting EHEC O157, and belongs to the technical field of bacterial examination. According to the preparation and applications, a high-sensitivity and high-specificity method for polymerase chain reaction in a nucleic acid test is combined with an immune colloidal gold quick testing technology in immunological detection, specific amplification is carried out on a target DNA by virtue of a unique marked primer pair, and an amplification product is combined with a gold labeled antibody fixed on a test paper strip so as to form a stable and visible test band and a stable and visible quality control band, and thus the quick and accurate testing on Escherichia coli O157:H7 can be realized.

The invention belongs to the technological field of medicine bioengineering, which more particularly relates to a monoclonal antibody neutralizing enterohemorrhagic escherichia coli O157:H7 shiga toxin II, Fab antibody sequences and the application. The monoclonal antibody is prepared by hybridoma cell lines with the preservation number of CCTCC: C200822. The antibody of the invention can be used for preparing medicines for diagnosing and treating EHEC O157 infections and the complicating diseases thereof.

The invention relates to the technical field of a biological preparation, and discloses lactobacillus gasseri for resisting enterohemorrhagic escherichia coli, an application of the lactobacillus gasseri, a composite probiotic preparation for poultry, prepared from the lactobacillus gasseri and an application of the composite probiotic preparation. For the lactobacillus gasseri (Lactobacillus gasseri) YD2-2 provided by the invention, the preservation No. is CCTCC NO:M 2019340, the lactobacillus gasseri particularly has efficient bacterial inhibition for the enterohemorrhagic escherichia coli,after the lactobacillus gasseri is mixed and blended with other bacterial strains, the composite probiotic preparation for poultry for resisting enterohemorrhagic escherichia coli can be prepared. Thepreparation can promote the growth of animals, the microbiological environment of intestines of the animals can be improved, the development of intestine mucosa can be promoted, the solubility IgA content and the serum IgG content of the intestine mucosa can be increased, the long-term sub-therapeutic dose and ultrahigh dose of antibiotics for preventing and treating animal diseases in feeds canbe reduced, and the damage in respects of bacterial drug resistance, drug residues of antibiotics, food safety and the like caused by indiscriminate use of the antibiotics can be avoided.

The invention discloses a enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 recombinant protein, including 1) enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 and expression vector fragment; or 2) enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunitactive fragment Stx2a1 protein carboxyl terminal acquires protein and expression vector fragment with similar function by losing and adding one or more amino terminals. The invention also discloses a preparation method of aforementioned enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 recombinant protein, application thereof in preparation of a test kit and preparation of subunit vaccine for prevention and cure infection of EHEC O157:H7.

The invention belongs to the technical field of animal health and food inspection and relates to a primer group for detecting EHEC (enterohaemorrhagic escherichia coli) O157:H7 with LAMP (loop-mediated isothermal amplification) and a detection method. The primer group for LAMP detection comprises four specific primers including a forward inner primer FIP:SEQ ID NO.1, a reverse inner primer BIP:SEQ ID NO.2, a forward outer primer F3:SEQ ID NO.3 and a reverse outer primer B3:SEQ ID NO.4. The detection method comprises the following steps: 1, extracting a DNA of a to-be-detected sample; 2, preparing an LAMP detection reaction system; 3, performing an LAMP reaction with the DNA extracted in the step 1 as a template and identifying a result with a real-time turbidity method. The method is not limited by culture conditions, a target nucleic acid can be detected within 50 min, qualitative detection sensitivity can reach 3.45*10<4> CFU / mL and is 10 times higher than that obtained with common PCR, the reaction result is easy to observe, the cost is low, and rapid screening results can be provided for supervision and inspection of market products and emergency handling by supervision departments.

The present invention provides a polypeptide, called EspA, which is secreted by pathogenic E. coli, such as the enteropathogenic (EPEC) and enterohemorrhagic (EHEC) E. coli. The invention also provides isolated nucleic acid sequences encoding EspA polypeptide, EspA peptides, a recombinant method for producing recombinant EspA, antibodies which bind to EspA, and a kit for the detection of EspA-producing E. coli.

The present invention relates to a new isolated polypeptide nominated microcin S, isolated nucleic acid molecules encoding the microcin S polypeptide and primers and probes hybridizing to the nucleic acid molecules. The invention also relates to plasmids and cells comprising the nucleic acid molecules, an antibody binding to the polypeptide, compositions as well as methods for producing and using the polypeptides. The present invention further relates to medical uses for treating or preventing microbial infections, functional gastrointestinal disorders or treating a tumor. The invention further relates to a method for preserving food and a method for coating dressing material.

The invention provides a nano PCR (Polymerase Chain Reaction) kit for simultaneously detecting enterohemorrhagic escherichia coli (EHEC) O45 and O145 and application thereof and relates to the technical field of detection of the enterohemorrhagic escherichia coli. The kit comprises a first primer pair and a second primer pair; the first primer pair comprises primers as shown by SEQ ID No: 3 and SEQ ID No: 4; and the second first primer pair comprises primers as shown by SEQ ID No: 7 and SEQ ID No: 8. The invention further provides the application of the kit to detection of the enterohemorrhagic escherichia coli O45 and O145 in a manner of not taking diagnosis as a target. The nano PCR kit provided by the invention can be used for rapidly and specifically simultaneously detecting two bloodserum types of EHEC in a sample and has a good application prospect.

The present invention provides methods and compositions for production of gram-negative bacterial mutants that are defective in intestinal colonization capacity and sensitive to infection by bacteriophage P1. Thus the present invention provides immunogenic compositions for the prevention or attenuation of food- and water-borne illnesses associated with ingestion of bacteria such as enterohemorrhagic Escherichia coli.

The invention relates to a multiplex PCR rapid detection kit for common pathogenic bacteria in livestock and poultry meat and aquatic products, which comprises 10×PCR buffer, MgCl2, dNTP, primers and DNA polymerase. The detection method includes: extracting the template of the sample to be tested; adding 10×PCR buffer, MgCl2, dNTP, primers, DNA polymerase, template of the sample to be tested and ddH2O water into the PCR thin-walled tube, mixing it and placing it on the PCR instrument for amplification. increase; electrophoresis amplification products in the electrophoresis equipment, record the results; analyze and judge the results. The present invention has better specificity, and can complete the detection of enterohemorrhagic Escherichia coli, Listeria monocytogenes, Salmonella and Vibrio parahaemolyticus within 8 to 9 hours, which is better than conventional bacteriological diagnosis. Quick, easy and economical.

The invention provides a nano-PCR kit for simultaneously detecting enterohaemorrhagic Escherichia coli O45 and O145 and an application thereof, and relates to the technical field of detection of hemorrhagic Escherichia coli. The test kit includes a first primer pair and a second primer pair, the first primer pair includes primers with sequences such as SEQ ID No: 3 and SEQ ID No: 4; the second primer pair includes primers with sequences such as SEQ ID No: Primers shown in ID No: 7 and SEQ ID No: 8. The present invention also provides the application of the nano-PCR kit in detecting enterohemorrhagic Escherichia coli O45 and O145 for the purpose of non-diagnosis. The nano-PCR kit of the invention can quickly and specifically detect two serotypes of EHEC in a sample at the same time, and has good application prospects.

The invention relates to a spice plant extract, and particularly relates to an ethanolic extract of cinnamons and an application thereof in preparing food preservatives; a natural food preservative is prepared by combining the ethanolic extract of cinnamons with a food additive; and the ethanolic extract of cinnamons provided by the invention has a good sterilization effect on various typical food-borne pathogenic bacteria, especially has a significant sterilization effect on the pathogenic bacteria O157: H7 of EHEC (enterohaemorrhagic escherichia coli, EHECO157: H7) food poisoning continuously occurring around the world in recent years, and has no side effects on food, therefore, the ethanolic extract of cinnamons is a natural food preservative which is safe and has no side effects; the ethanolic extract of cinnamons is widely applied to the decomposition prevention of products such as fresh vegetables and fruits and beef hamburgers and the like; and in the aspect of freshness retaining, the ethanolic extract of cinnamons not only can effectively prolong the shelf life of food, but also can effectively prevent the occurrence of bacterial food poisoning and effectively ensure the safety of food, therefore, the ethanolic extract of cinnamons plays an important role in the field of food health and safety.

The superiority prior antibiotic strain was isolated. Feeding the isolated strain to ruminants prevents colonization of the animals by the pathogen E. coli 0157:H7. The strain was reisolated from the contents of the gastrointestinal tract of vaccinated animals 28 days after inoculation. Animals previously infected with E. coli 0157:H7 can be treated to reduce or eliminate the pathogen by administering any of the advantageous prior antibiotic bacteria of the present invention.