42 results about "Enterohemorrhagic e coli" patented technology

The present invention relates to chemically modified hollow fiber materials for extracorporeally removing exotoxins produced by pathogenic Escherichia coli from protein containing liquids, particularly from blood or plasma, as well as the use in treating patients suffering from diseases which, for example, are caused by enterohemorrhagic Escherichia coli bacteria (EHEC infections) or other pathogenic Escherichia coli like enterotoxigenic Escherichia coli (ETEC) or enteroaggregative Escherichia coli (EAEC).

The invention discloses a enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 recombinant protein, including 1) enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 and expression vector fragment; or 2) enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunitactive fragment Stx2a1 protein carboxyl terminal acquires protein and expression vector fragment with similar function by losing and adding one or more amino terminals. The invention also discloses a preparation method of aforementioned enterohemorrhagic escherichia coli O157:H7 shiga toxin 2A1 subunit active fragment Stx2a1 recombinant protein, application thereof in preparation of a test kit and preparation of subunit vaccine for prevention and cure infection of EHEC O157:H7.

The present invention provides methods and compositions for production of gram-negative bacterial mutants that are defective in intestinal colonization capacity and sensitive to infection by bacteriophage P1. Thus the present invention provides immunogenic compositions for the prevention or attenuation of food- and water-borne illnesses associated with ingestion of bacteria such as enterohemorrhagic Escherichia coli.

The invention relates to a multiplex PCR rapid detection kit for common pathogenic bacteria in livestock and poultry meat and aquatic products, which comprises 10×PCR buffer, MgCl2, dNTP, primers and DNA polymerase. The detection method includes: extracting the template of the sample to be tested; adding 10×PCR buffer, MgCl2, dNTP, primers, DNA polymerase, template of the sample to be tested and ddH2O water into the PCR thin-walled tube, mixing it and placing it on the PCR instrument for amplification. increase; electrophoresis amplification products in the electrophoresis equipment, record the results; analyze and judge the results. The present invention has better specificity, and can complete the detection of enterohemorrhagic Escherichia coli, Listeria monocytogenes, Salmonella and Vibrio parahaemolyticus within 8 to 9 hours, which is better than conventional bacteriological diagnosis. Quick, easy and economical.

The invention provides a nano-PCR kit for simultaneously detecting enterohaemorrhagic Escherichia coli O45 and O145 and an application thereof, and relates to the technical field of detection of hemorrhagic Escherichia coli. The test kit includes a first primer pair and a second primer pair, the first primer pair includes primers with sequences such as SEQ ID No: 3 and SEQ ID No: 4; the second primer pair includes primers with sequences such as SEQ ID No: Primers shown in ID No: 7 and SEQ ID No: 8. The present invention also provides the application of the nano-PCR kit in detecting enterohemorrhagic Escherichia coli O45 and O145 for the purpose of non-diagnosis. The nano-PCR kit of the invention can quickly and specifically detect two serotypes of EHEC in a sample at the same time, and has good application prospects.

The invention discloses an engineering lambda bacteriophage capable of specifically killing enterohemorrhagic Escherichia coli and a construction method and application thereof, the engineering lambda bacteriophage is capable of specifically removing enterohemorrhagic Escherichia coli in a complex microbial environment, and a lyase gene of the engineering lambda bacteriophage is knocked out and is loaded with a CRISPR-Cas3 system. The CRISPR-Cas3 system plays a role in killing enterohemorrhagic Escherichia coli with high specificity by utilizing the soluble source characteristic of the engineering lambda bacteriophage, so that the defect of broad-spectrum sterilization of antibiotic and wild bacteriophage treatment is effectively overcome.

The invention relates to a spice plant extract, and particularly relates to an ethanolic extract of cinnamons and an application thereof in preparing food preservatives; a natural food preservative is prepared by combining the ethanolic extract of cinnamons with a food additive; and the ethanolic extract of cinnamons provided by the invention has a good sterilization effect on various typical food-borne pathogenic bacteria, especially has a significant sterilization effect on the pathogenic bacteria O157: H7 of EHEC (enterohaemorrhagic escherichia coli, EHECO157: H7) food poisoning continuously occurring around the world in recent years, and has no side effects on food, therefore, the ethanolic extract of cinnamons is a natural food preservative which is safe and has no side effects; the ethanolic extract of cinnamons is widely applied to the decomposition prevention of products such as fresh vegetables and fruits and beef hamburgers and the like; and in the aspect of freshness retaining, the ethanolic extract of cinnamons not only can effectively prolong the shelf life of food, but also can effectively prevent the occurrence of bacterial food poisoning and effectively ensure the safety of food, therefore, the ethanolic extract of cinnamons plays an important role in the field of food health and safety.

The superiority prior antibiotic strain was isolated. Feeding the isolated strain to ruminants prevents colonization of the animals by the pathogen E. coli 0157:H7. The strain was reisolated from the contents of the gastrointestinal tract of vaccinated animals 28 days after inoculation. Animals previously infected with E. coli 0157:H7 can be treated to reduce or eliminate the pathogen by administering any of the advantageous prior antibiotic bacteria of the present invention.