The invention provides a method for sterilizing and preserving fresh mulberry fruits, and belongs to the technical field of fruit preservation, the method comprises the following steps: 1) spreading the fresh mulberry fruits in a closed container in a single layer; and 2) introducing atmospheric plasma into the closed container, and sterilizing to obtain the sterilized fresh mulberry fruits. According to the method disclosed by the invention, bacteria, yeasts and fungi, such as botrytis cinerea, salmonella, escherichia coli, staphylococcus aureus and bacillus cereus, on the surfaces of the mulberries can be remarkably inactivated. The method has no significant influence on the quality of the mulberries, such as pH, TSS, hardness and color, and can significantly inhibit the rotting rate and mildew rate of the mulberries, and the rotting rate can be reduced by 30.00% and the mildew rate can be reduced by 25.14% at most.