Bacillus subtilis strain and method for preparing Pu'er tea capable of regulating intestinal functions from same

A technology of Bacillus subtilis and Pu-erh tea, applied in the field of microorganisms and food biology, can solve problems such as increasing the chance of food-borne pathogenic bacteria contamination, and achieve the effects of assisting in regulating intestinal function, promoting growth and genetic stability.

Pending Publication Date: 2018-12-14
CHINA TEA YUNAN CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the fermentation process of Pu-erh tea, some miscellaneous bacteria that are not conducive to Pu-erh fermentation may enter, and if it is stored in a high-humidity and high-temperature environment in the later stage, it will increase the chance of contamination by food-borne pathogens

Method used

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  • Bacillus subtilis strain and method for preparing Pu'er tea capable of regulating intestinal functions from same

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Embodiment 1

[0041] Embodiment 1 patent Pu'er tea

[0042] According to the method of the present invention, a single-strain fermentation process is adopted to prepare Pu'er ripe tea by using Bacillus subtilis (CGMCC No.15959). Proceed as follows:

[0043] 1. After blending and mixing different raw materials according to the requirements, it is required that the tea leaves have no flowers after mixing;

[0044] 2. Inoculate the prepared liquid seeds into the tea raw materials, the inoculum amount is 28%, the bacteria liquid is required to be sprayed evenly, the tea leaves are not agglomerated, and the density of the liquid seeds is 5*10 7 cfu / mL

[0045] 3. Stack the inoculated tea leaves into a small tea pile with a height of 0.5 meters and a length and width of 1*1 meter, and carry out pile fermentation;

[0046] 4. Turn the pile once every 5 days to allow the core tea leaves to mix evenly with the opposite pile surface tea leaves, and maintain the core temperature and moisture conten...

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Abstract

The invention specifically relates to a Bacillus subtilis strain and a method for preparing Pu'er tea capable of regulating intestinal functions from the same, belonging to the field of microbes and food biotechnology. The Bacillus subtilis strain has been preserved in China General Microbiological Culture Collection Center, with an accession number is CGMCC No. 15959. The Bacillus subtilis strainof the invention is applied to the processing technology for Pu'er tea, and can effectively inhibit the growth of other harmful microorganisms, including Staphylococcus aureus, Salmonella, Aspergillus flavus and other toxin-producing fungi. The Pu'er tea prepared from the Bacillus subtilis strain is capable of regulating intestinal functions.

Description

technical field [0001] The invention belongs to the field of microorganism and food biotechnology, and relates to a strain of Bacillus subtilis 0061-BS and its application in Pu'er tea processing technology, and the regulation of intestinal function of tea. Background technique [0002] Pu-erh tea is made from Yunnan big-leaf sun-dried green tea, and is considered to be the Chinese tea with the most variety and stories. Ripe Pu-erh tea is a post-fermented tea, which is made from sun-dried green tea after more than one month of heap fermentation. During the fermentation process of Pu-erh tea, some miscellaneous bacteria that are not conducive to Pu-erh fermentation may enter, and if it is stored in a high-humidity and high-temperature environment in the later stage, the chance of contamination by food-borne pathogenic bacteria will increase. [0003] Bacillus subtilis is a kind of aerobic bacillus, which exists in soil and nature, has no pathogenicity, and can secrete a vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A23F3/10C12R1/125
CPCA23F3/06A23F3/10A23F3/14C12N1/02C12N1/14C12N1/20C12N1/145C12N1/205C12R2001/125C12R2001/645C12R2001/66C12R2001/685
Inventor 魏珍珍邹广田盛玉泊官云平郝彬秀李颂孙婷婷田海霞周方勇顾红惠
Owner CHINA TEA YUNAN CO LTD
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