Method for extracting components having antibacterial activity from fruit of pedunculate herpetospermum

A kind of technology of antibacterial activity, wave ling melon, applied in the field of extracting antibacterial effective components

Inactive Publication Date: 2011-04-06
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the antibacterial activity of Boling melon at home and abroad, so the present invention stud

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take the watermelon seeds, dry and pulverize them, use 5 times the amount of 95% ethanol, and extract 3 times at room temperature, each soaking time is 12 hours; combine the filtrates, concentrate under reduced pressure to obtain an extract; suspend the extract in deionized water, Degrease with petroleum ether (petroleum ether: water, V / V=1:1) 5 times until the petroleum ether layer is light yellow; extract the degreased water layer with ethyl acetate (V / V=1:1), 3 times, the combined extracts were concentrated under reduced pressure to obtain extracts; the extracts were subjected to silica gel column chromatography, and the silica gel column chromatography was normal phase silica gel column chromatography; the normal phase of the mobile phase used was petroleum ether-ethyl acetate, and the ratio was 100 :0, 100:1, 50:1, 30:1, 20:1, 10:1, 8:1, 5:1, 2:1, 1:1, combine effluents according to eluent polarity, concentrate 10 components (Fr.1-Fr.10) were obtained, and the conc...

Embodiment 2

[0017] Take the watermelon seeds, dry and pulverize them, use 8 times the amount of 75% ethanol, extract 4 times at room temperature, and soak for 24 hours each time; combine the filtrates, concentrate under reduced pressure to obtain extracts; suspend the extracts in deionized water, and use Petroleum ether (petroleum ether: water, V / V=1:4) was degreased 6 times until the petroleum ether layer was light yellow-green; the water layer after degreasing was washed with ethyl acetate (ethyl acetate / water, V / V=1 / 2) Carry out extraction, 6 times, combine the extracts, concentrate under reduced pressure to obtain the extract; the extract is subjected to silica gel column chromatography, and the silica gel column chromatography is normal phase silica gel column chromatography; the normal phase of the mobile phase used is petroleum ether-ethyl acetate Esters, ratios 100:0, 100:1, 50:1, 30:1, 20:1, 10:1, 8:1, 5:1, 2:1, 1:1, according to eluent polarity The effluents were combined and c...

Embodiment 3

[0019] Take pollen melon seeds, dry and pulverize them, use 10 times the amount of 75% methanol, extract 5 times at room temperature, and soak for 24 hours each time; combine the filtrates, freeze-dry and concentrate them into extracts; suspend the extracts in deionized water, and Diethyl ether (ether / water, V / V=1:2) was degreased 5 times until the ether layer was light yellow-green; the degreased water layer was extracted with chloroform (chloroform / water, V / V=1:1) 5 times , combined extracts, concentrated under reduced pressure to be medicinal extracts; the medicinal extracts were subjected to silica gel column chromatography, which was normal phase silica gel column chromatography; the normal phase of the mobile phase used was petroleum ether-ethyl acetate, and the ratio was 100:0 , 100:1, 50:1, 30:1, 20:1, 10:1, 8:1, 5:1, 2:1, 1:1, according to the polarity of the eluent, the effluents were combined and concentrated to obtain 10 components (Fr.1-Fr.10), the concentrate is ...

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Abstract

The invention belongs to the technical field of research and development on medical products. The invention applies for the protection of a method for extracting components having antibacterial activity from fruit of pedunculate herpetospermum which is a Tibetan medicine. The method comprises: extracting coarse extract from the dried and crushed fruit of pedunculate herpetospermum by using a solvent and a solid-liquid extraction method, and concentrating the coarse extract to obtain extract; obtaining extract with obvious antibacterial effect by degreasing, concentrating and extracting; and further separating the extract by silica gel column chromatography and obtaining the effective extract components. The product of the invention has different inhibition effects on golden staphylococcus, tritirachium album, bacillus subtilis and salmonella, can be developed as a natural antibacterial to be used in medical industry and can be prepared into antibacterial tablets, granules, capsules, injection, skin external preparations and other formulations. Thus, a new field of the development of the pedunculate herpetospermum fruit Tibetan medicine is opened.

Description

technical field [0001] The invention relates to a method for extracting antibacterial effective components from pollen melon seeds by solvent extraction, extraction and silica gel column chromatography, and belongs to the technical field of traditional Chinese medicines. Background technique [0002] Boling melon is also known as Sejimedo and Seme Segu (Herpetosperm umpedunculosum Baill1), is an annual climbing vine of Cucurbitaceae Bolinggua. It is mainly distributed in the southeast of Tibet at an altitude of 2600-3000m, near forest edges and villages. Boling melon seeds are the dried seeds of Cucurbitaceae Boling melon. It tastes bitter and cold in nature. It has the effects of clearing heat and detoxifying, softening the liver and purging liver fire, and relieving gallbladder heat. It is used to treat red bar disease, liver disease, gallbladder disease and digestion bad. "Jingzhu Materia Medica" contains: "Boling melon seeds are cool and sharp in nature, and they can t...

Claims

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Application Information

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IPC IPC(8): A61K36/42A61P31/04A61K131/00
CPCY02A50/30
Inventor 陈敏廖志华兰小中徐冰
Owner SOUTHWEST UNIVERSITY
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