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Preparation method of acyclovir-chitosan nanoparticles

A technology of chitosan nanoparticles and chitosan, which is applied in the directions of inactive medical preparations, pharmaceutical formulations, inorganic inactive ingredients, etc., to enhance antiviral effect, slow down and stabilize the release rate, and improve bioavailability Effect

Inactive Publication Date: 2011-03-30
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports of nanoparticle sustained-release preparations loaded with acyclovir using chitosan and gelatin as carriers.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Dissolve chitosan with a relative molecular weight of 200,000 and a degree of deacetylation of 92% with 1% acetic acid to obtain a chitosan solution with a concentration of 2.5 mg / mL, and then dissolve the model drug acyclovir in the shell In the polysaccharide solution, the concentration of acyclovir was 2.5mg / mL; then 10mL of 1.5mg / mL sodium tripolyphosphate solution was added to 20mL of acyclovir-chitosan solution, at 25°C Stir at 800rpm for 30 minutes to make nanoparticle suspension. The suspension was centrifuged at 20,000 rpm for 30 minutes, and finally the precipitate was vacuum freeze-dried for 10 hours to obtain acyclovir-chitosan nanoparticles. Add the obtained nanoparticles to the gelatin aqueous solution with a concentration of 4mg / mL, stir at 800rpm for 30 minutes at 25°C, centrifuge the liquid at 20,000rpm for 30 minutes, and finally vacuum freeze-dry the precipitate for 10 hours , to obtain acyclovir chitosan-gelatin nanoparticles. The encapsulation eff...

Embodiment 2

[0017] Dissolve chitosan with a relative molecular weight of 200,000 and a degree of deacetylation of 92% with 1% acetic acid to obtain a chitosan solution with a concentration of 2.5 mg / mL, and then dissolve the model drug acyclovir in the shell In the polysaccharide solution, the concentration of acyclovir was 2.0mg / mL; then 10mL of 1.5mg / mL sodium tripolyphosphate solution was added to 20mL of acyclovir-chitosan solution, at 25°C Stir at 800rpm for 30 minutes to make nanoparticle suspension. The suspension was centrifuged at 20,000 rpm for 30 minutes, and finally the precipitate was vacuum freeze-dried for 10 hours to obtain acyclovir-chitosan nanoparticles. Then add the obtained nanoparticles to the gelatin aqueous solution with a concentration of 6 mg / mL, stir at 800 rpm for 30 minutes at 25 ° C, centrifuge the liquid at 20,000 rpm for 30 minutes, and finally vacuum freeze-dry the precipitate for 10 hours , to obtain acyclovir chitosan-gelatin nanoparticles. The encapsu...

Embodiment 3

[0019] Dissolve chitosan with a relative molecular weight of 200,000 and a degree of deacetylation of 92% with 1% acetic acid to obtain a chitosan solution with a concentration of 2.0 mg / mL, and then dissolve the model drug acyclovir in the shell In the polysaccharide solution, the concentration of acyclovir was 1.0mg / mL; then 10mL of 1.0mg / mL sodium tripolyphosphate solution was added to 20mL of acyclovir-chitosan solution, at 25°C Stir at 800rpm for 30 minutes to make nanoparticle suspension. The suspension was centrifuged at 20,000 rpm for 30 minutes, and finally the precipitate was vacuum freeze-dried for 10 hours to obtain acyclovir-chitosan nanoparticles. Add the obtained nanoparticles to the gelatin aqueous solution with a concentration of 8mg / mL, stir at 800rpm for 30 minutes at 25°C, centrifuge the liquid at 20,000rpm for 30 minutes, and finally vacuum freeze-dry the precipitate for 10 hours , to obtain acyclovir chitosan-gelatin nanoparticles. The encapsulation eff...

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Abstract

A preparation method of acyclovir-chitosan nanoparticles comprises the following steps: dissolving acyclovir in chitosan acetum, dropping aqueous solution of sodium tripolyphosphate as a crosslinking agent, obtaining drug-loading chitosan nanoparticles after centrifuging and freeze drying, dropping the chitosan nanoparticles in aqueous solution of gelation, and obtaining chitosan-gelation nanoparticles containing acyclovir after centrifuging and freezing drying. The preparation method has the advantage of improving the delayed release effect of medicine by embedding medicine in particles in embedding manner; and the preparation method has simple preparation technology and low cost, and is easy for large-scale production.

Description

technical field [0001] The invention belongs to a preparation method of acyclovir-chitosan nanoparticles. Background technique [0002] Compared with the drugs of ordinary preparations, nano-medicines have larger specific surface area, stronger chemical activity, and faster absorption speed. In many aspects, such as sex, nano-medicine has irreplaceable advantages of general medicine. [0003] Chitosan is the only polycationic natural polymer material in nature. It is a compound extracted from the shells of shrimps and crabs. It has good biocompatibility, biodegradability, good film-forming properties, and also has antibacterial properties. active. Gelatin is the collagen extracted from the bones and skins of cattle, pigs and other animals through a series of pretreatment and purification. It has the dual characteristics of protein and material, and it is a hemostatic agent in medicine, and it also has good biocompatibility and biodegradability. Chitosan and gelatin are b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K47/48A61K47/42A61K47/36A61K47/02A61K31/522A61P31/12
Inventor 李思东章超桦杨磊权维燕欧春艳董静静问莉莉廖双泉郑彦丽钟海涛
Owner GUANGDONG OCEAN UNIVERSITY
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