Development culture medium for separating and identifying pathogens in urogenital tract

Abstract

The invention discloses a development culture medium for separating and identifying pathogens in a urogenital tract, which at least comprises three development substrates, namely a hexosaminidase substrate, a beta-D-galactosidase substrate, a beta-D-glucuroide substrate. The development culture medium added with the three development substrates is prepared into a microbial culture medium agar plate, a sample or a bacterial colony subjected to separate culture is inoculated into the development plate and is incubated, and a result can be directly observed. In the development culture medium, various pathogens including bacteria and fungi in a genital tract can be simultaneously cultured and identified on the same development plate, namely bacterial pathogens such as Escherichia coli, Klebsiella pneumonia, enterococcus, pseudomonas aeruginosa, proteus mirabilis, staphylococcus aureus and the like and fungal pathogens such as candida albicans, candida tropicalis and the like can be simultaneously identified, and judgment can be performed through visual inspection; and the development culture medium is quick and convenient to use, and easy to operate.

Description

technical field [0001] The invention relates to a chromogenic medium for detecting pathogenic bacteria, in particular to a chromogenic medium for the isolation and identification of urogenital pathogenic bacteria, which can simultaneously identify various bacterial and fungal pathogenic bacteria. Background technique [0002] Urogenital tract infection refers to pathogens directly invading the urinary tract or reproductive tract, growing and multiplying in the urethral or vaginal environment, and invading mucous membranes or tissues to cause damage. [0003] The bacterial pathogens that cause urogenital tract infections are mainly Gram-negative bacilli, among which Escherichia coli is the main one, accounting for about 50-70%, and Klebsiella, Proteus, Pseudomonas In recent years, genitourinary tract infections caused by Gram-positive bacteria are on the rise, mainly Enterococcus and coagulase-positive Staphylococcus aureus, while the infection rates of coagulase-negative Sta...

AI Technical Summary

Problems solved by technology

[0009] ④. UTI is defined as a chromogenic medium: it can perform localization analysis on Escherichia coli, Klebsiella pneumoniae, Enterococcus, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, etc., but cannot separate and identify Candida, etc. ;

[0011] At present, the traditional method is the main method for culturing and identifying pathogenic bacteria on patient specimens in clinical practice. Multiple steps such as isolation and culture, microscopic observation, and biochemical identification are adopted. The detection cycle is long and the operation is cumbersome. The need for convenient detection, although there are currently several chromogenic media for the detection of urinary tract bacteria and Candida on the market, such as CHROMagar localization chromogenic medium, Mérieux localization chromogenic medium, CHROMagar Candida However, these chromogenic media can only be used to detect bacterial pathogens, or can only be used to detect Candida, which cannot meet the simultaneous isolation and identification of bacterial pathogens and Candida that cause reproductive tract infections, and there are loopholes. It is not conducive to the comprehensive clinical judgment of pathogenic bacteria, thus affecting the diagnosis and treatment of diseases

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