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383 results about "Enterobacterales" patented technology
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Probiotic recolonisation therapy
Owner:FINCH THERAPEUTICS HLDG LLC
Antibacterial fabric finishing agent containing chitose its production and usage
InactiveCN101074541AIncrease anchorageImprove antibacterial propertiesFibre treatmentAcetic acidEscherichia coli
One finishing solution of fabrics containing chitosan is composed of Chitosan (MW <= 5000, the degree of deacetylation of >= 90%, 1.00 - 5.00%), acetic acid (0.50 - 6.00%), epichlorohydrin (0.01 - 1.00%) and water. The process for the fabric finishing is: I,fabric Pretreatment: fabric by the alkali treatment (8 - 11% NaOH at room temperature), washed, acid and (2 - 4% acetic acid at room temperature), drying (85 - 100 deg.C). II,finishing: baptist, rolling (room temperature, 15 to 25 min, rolling rate of more than 80 - 90%), alkali (room temperature, 5 - 8% NaOH), acid and (room temperature, 1 to 3% acetic acid) and Drying (85 - 100 deg.C). The test results show that the inhibitory rate for staphylococcus aureus, Escherichia coli, Streptococcus white Nian is more than 99% which is the higher level compared to the state 3-A class standard. The fastness of fabrics reaches 4 after washing.
Owner:大连利健甲壳素股份有限公司
Oligonucleotide primer for detecting common pathogenic bacteria by adopting fluorescent quantitation PCR (Rich Client Platform) technology, method thereof for detecting common pathogenic bacteria and application thereof
InactiveCN101928773AEfficient and wideWide range of applicationsMicrobiological testing/measurementAgainst vector-borne diseasesBiotechnologyFood poisoning
The invention discloses an oligonucleotide primer for detecting common pathogenic bacteria by adopting a fluorescent quantitation PCR (Rich Client Platform) technology, a method thereof for detecting common pathogenic bacteria and the application thereof. The method comprises the following steps of: providing 10 pairs of specific oligonucleotide primer sequences at annealing temperature of 50-60 DEG C without differing 5 DEG C; and simultaneously, quickly, accurately and effectively identifying and quantificationally detecting various pathogenic bacteria at the same time. A detection range comprises bacillus cereus, enterobacter sakazakii, vibrio parahaemolyticus, enterohemorrhagic escherichia coli O157, salmonella, Listeria monocytogenes, Shigella, campylobacter jejuni, pseudomonas aeruginosa, klebsiella pneumoniae, and the like. The invention also can be used for the fields of disease diagnosis, environmental monitoring, water-quality and food supervision and detection, food poisoning pathogenicbacteria detection, bacteriological classification, epidemiological investigation, biological agent detection, and the like, is convenient, quick, accurate and effective and has wide application range.
Owner:INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
Niacinamide ribose phosphate transferase for preparing NMN, coding gene, recombinant vector and application
ActiveCN109666658ASimple processReduce investmentBacteriaMicroorganism based processesEscherichia coliNiacinamide
Owner:成都及禾生物科技有限公司
Ethane-1,2-diol producing microorganism and a method for producing ethane-1,2-diol from d-xylose using the same
Disclosed herein is a microorganism capable of producing ethane-1,2-diol from D-xylose, and a method for producing ethane-1,2-diol using the same. More specifically, the present invention relates to an engineered Escherichia coli (E. coli) prepared by knocking out a D-xylose isomerase gene and / or an aldehyde dehydrogenase gene within the genomic DNA of E. coli and transforming an expression vector including a D-xylose dehydrogenase gene into the E. coli, and an efficient method for producing ethane-1,2-diol from D-xylose using the engineered E. coli.
Owner:MYONGJI UNIV IND & ACAD COOPERATION FOUND
Pseudomonas aeruginosa bacteriophage endolysins, and coding gene and application thereof
InactiveCN108410840AGood water solubilityHigh activityAntibacterial agentsPeptide/protein ingredientsEscherichia coliProtein
The invention discloses pseudomonas aeruginosa bacteriophage endolysins, and a coding gene and application thereof. The invention provides a protein which is as shown in (a) or (b) as below: (a) the protein which is as shown in a sequence 1 in a sequence table and consists of an amino acid sequence; (b) the protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence which is as shown in the sequence 1 in the sequence table, has a function of cracking gram-negative bacteria and is deviated from the sequence 1 in the sequencetable. The protein provided by the invention can crack a plurality of gram-negative bacteria, such as Escherichia coli, Klebsiella pneumonia, pseudomonas aeruginosa, Burkholderia cepacia and Salmonella typhimurium. The protein and a surfactant EDTA (Ethylene Diamine Tetraacetic Acid) act synergistically to achieve a stronger inhibiting effect on the gram-negative bacteria. A foundation is laid for researching and developing a new antibacterial preparation.
Owner:DALIAN UNIV OF TECH
Chitosan quaternary ammonium salt derivatives with good water solubility and antibacterial activity and preparation method of chitosan quaternary ammonium salt derivatives
The invention relates to five chitosan quaternary ammonium salt derivatives with good water solubility and antibacterial activity and a preparation method of the chitosan quaternary ammonium salt derivatives, and belongs to the technical fields of chitosan antibacterial materials and preparation methods thereof. The preparation method comprises the following steps: reacting mono monosulfate ester of N-methyl-N-long-chain alkyl-N,N-2-hydroxyethyl ammonium halide with amino-protected N-benzylidene-chitosan, and carrying out amino deprotection reaction to obtain an O-quaternary ammonium salt-chitosan derivative. The five chitosan quaternary ammonium salt derivatives are simple in synthesis, high in substitution degree, low in cost, and stable in property, and has good water solubility and antibacterial activity and low cytotoxicity; and the purification method is simple and convenient; and the five chitosan quaternary ammonium salt derivatives provided by the invention have relatively good antibacterial capability on kinds of germs such as such as staphylococcus aureus and escherichia coli and various fungi such as aspergillus niger, are suitable for serving as antibacterial materials, medicine carriers and medical equipment materials, and have good application prospects.
Owner:BINZHOU MEDICAL COLLEGE
Hermetia illucens L antibacterial peptide, preparation method and application thereof
InactiveCN102816769AHas broad-spectrum antimicrobial activitySimple methodAntibacterial agentsBiocideHermetiaTotal rna
The present invention discloses a new Hermetia illucens L antibacterial peptide StomoxynZH1, a preparation method and an application thereof. Preparation steps of the antibacterial peptide comprise: extracting the total RNA of insect Hermetia illucens L as a template, performing RT-PCR to obtain a first strand of cDNA as a template, and performing PCR amplification to obtain a PCR product; carrying out digestion to obtain a PCR product and a eukaryotic expression vector to construct a recombinant expression vector; transforming the resulting recombinant expression vector into yeast host cellsto obtain a recombinant strain expressing the antibacterial peptide StomoxynZH1; and carrying out inducing culture on the resulting recombinant strain to obtain a culture, and carrying out centrifugal separation on the culture to obtain the expressed antibacterial peptide. The antibacterial peptide of the present invention has broad spectrum antimicrobial activities and provides good application prospects in medicine, agriculture disease prevention and control, feed additives and other fields, wherein the broad spectrum antimicrobial activities comprise gram-negative bacteria escherichia coliresistance, gram-positive bacteria staphylococcus aureus resistance, and fungi rice blast pathogenic bacteria resistance.
Owner:HUAZHONG AGRI UNIV
Multiplex PCR-based synchronous and rapid method for detecting 13 pathogenic microorganisms in water
InactiveCN102703588AStrong specificityHigh sensitivityMicrobiological testing/measurementAgainst vector-borne diseasesYersinia pestisEnterobacterales
The invention relates to a multiplex PCR-based synchronous and rapid method for detecting 13 pathogenic microorganisms in water, which comprises the steps of using multiplex PCR to simultaneously amplify gene-specific fragments of the 13 pathogenic microorganisms including escherichia coli, enterohaemorragic escherichia coli o157:h7, legionella pneumophila, salmonella enteritidis, shigella dysenteriae, staphyloccocus aureus, listeria monoeytogenes, helicobacter pylori, mycobacterium tuberculosis, klebsiella pneumonia, vibrio cholera, bacillus anthracis and yersinia pestis, and detecting PCR amplified products through agarose gel electrophoresis, thereby achieving synchronous and rapid detection for the 13 pathogenic microorganisms.
Owner:LOGISTICAL ENGINEERING UNIVERSITY OF PLA +1
N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound as well as synthetic method and application thereof
InactiveCN103951566AHigh antibacterial activityGood antitumor activityAntibacterial agentsAntimycoticsCandida tropicalisCancer cell proliferation
The invention discloses an N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound as well as a synthetic method and an application thereof. Longifolene serving as a heavy oil main component is relatively rich terpenoid in numerous natural extracts. The synthetic method comprises the following steps: obtaining isolongifolene through longifolene isomerization, obtaining isolongifolenone through allylic oxidation of the isolongifolene, carrying out dehydration condensation on the isolongifolenone and primary amine so as to obtain imine and obtaining the chiral N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound after reduction. The compound has vey good inhibition activity on candida albicans, aspergillus niger, candida tropicalis, escherichia coli, staphylococcus aureus, pseudomonas fluorescens, bacillus subtilis and the like, a part of compound has good cancer cell proliferation inhibition activity on human breast cancer cells MCF-7, human lung adenocarcinoma cells A549, hepatoma cells HepG2 and SMMC-7721, and the N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound is a potential antibacterial and bactericidal and anti-tumor compound.
Owner:NANJING FORESTRY UNIV
Antimicrobial peptide with drug-resistance bacteria resistance activity and synthesis and application thereof
The invention designs antimicrobial peptide with drug-resistance bacteria resistance activity and a novel structure. The antimicrobial peptide is obtained by modifying amino acid at N tail ends of natural antimicrobial peptide Anoplin and MPI respectively, then performing acetylization on the amino acid, and performing intermolecular connection on every two side chains of the peptide by replacing a -1H-1,2,3-triazole structure by one 1,4- under the 1,3-dipolar cycloaddition reaction of the click chemistry. The antimicrobial experiments and pyridinium iodide dyeing method experiments for staphylococcus aureus and escherichia coli of the conventional gram-positive bacterium and clinically separated drug-resistant staphylococcus aureus and drug-resistant escherichia coli show that the J-AM peptide and the J-AA peptide which are synthesized by the method are higher in antimicrobial activity, higher in drug-resistance bacteria resistance activity and higher in cytomembrane film penetration capacity and has an obvious drug-resistance advantage of avoiding a drug-resistance phenomenon caused by the general antibiotics; and therefore, the antimicrobial peptide has an extremely high application prospect in preparation of clinical treatment medicines.
Owner:LANZHOU UNIVERSITY
Loop-mediated isothermal amplification detection primer groups of Escherichia coli 0157, detection method and reagent kit
ActiveCN102943113AHigh sensitivityShort detection timeMicrobiological testing/measurementDNA/RNA fragmentationEscherichia coliFood borne
The invention discloses loop-mediated isothermal amplification detection primer groups of Escherichia coli 0157, a detection method and a reagent kit. A set of specific detection primer groups, the detection reagent kit containing detection primer groups, the method using the detection reagent kit for detection through loop-mediated isothermal amplification and the detection method for determining whether the Escherichia coli 0157 exists in a sample to be tested are designed and screened for rfbE genes of the Escherichia coli 0157. The detection reagent kit and the detection method have the advantages that the sensitivity is high, the specificity is high, the accuracy rate reaches 99% and the detection time is short, only 8-10 hours are spent from sample treatment to result reporting, no polymerase chain reaction instrument or electrophoresis apparatus is required, the operating process is simple, the problems that traditional detection methods are long in consumed time, traditional cultural methods are low in sensitivity, cross reaction exists during serum agglutination and the like are solved, the detection reagent kit and the detection method are significant to timely and effective handling of food-borne sudden public health events, and the detection reagent kit and the detection method are particularly applicable to self-checking of basic level detection mechanisms and food processing enterprises.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
Lactobacillus paracasei subsp. tolerans strain, screening method and use
The invention relates to a Kefir source Lactobacillus paracasei subsp. tolerans strain with antimicrobial properties and its bacteriostatic composition. The strain FX-6-1 provided by the invention is preserved in China Center for Type Culture Collection on Feb. 23, 2014 with a preservation number of CCTCC M 2014043. The bacteriostatic composition generated by fermentation of the strain can inhibit the growth of Escherichia coli, Staphylococcus aureus and other bacterial pathogenic microorganisms, and Aspergillus flavus, Rhizopus nigricans and other moulds, and has a wide antibacterial spectrum. The bacteriostatic composition generated by fermentation of the strain provided by the invention shows stable properties on heat treatment and enzymolysis treatment, can be used as a preservative in feed and food, and has the potential of development into a medical composition.
Owner:SOUTH CHINA AGRI UNIV
Method for producing type 2 capsid protein grain vaccine of porcine circovirus by pichia pastoris expression system
InactiveCN103169961AImprove solubilityHigh expressionViral antigen ingredientsVirus peptidesEscherichia coliPichia pastoris
The invention provides a method for producing type 2 capsid protein grain vaccine of porcine circovirus by a pichia pastoris expression system. The method comprises the following steps of: based on a PCV2-TJ strain gene sequence in GenBank as a template, obtaining a mature coding sequence of PCV-2 capsid protein Cap by amplification by a normal PCR method; connecting the gene with a capsid protein secreting type expression carrier pP-secSUMO3 to construct a pP-secSUMO3-Cap recombinant plasmid and amplifying by a heat stock method to convert escherichia coli JMS115; purifying the positive recombinant plasmid and electrically converting pichia pastoris strain GS115 by an electric converter of BioRad company; sieving high copy strains according to concentration gradient of antibiotic ZeocinTm; and respectively extracting genomic DNAs of the high copy strains as the template to perform PCR reaction to identify positive clone. The piglet challenge test verifies that the grain vaccine has good immunoprotection effect.
Owner:BEIJING DAWEIJIA BIOTECH SHARE CO LTD
Detection assembly for detecting bacteria
InactiveCN104297474AAvoid pairing problemsHigh sensitivityMaterial analysisBiotechnologySpecific antibody
The invention relates to a detection assembly for detecting bacteria based on a bacteria fluorescent staining technique. A single antibody is utilized for detecting thalli which are stained by fluorescence; an escherichia coli O157:H7 antibody, a listeria monocytogenes antibody and a salmonella typhimurium antibody are sprayed on a test strip, and the test strip acts an immunochromatography test strip with three detection lines (T1, T2 and T3 lines). The detection assembly for detecting bacteria has the advantages that fluorochrome is used for pre-staining thalli, and the respective specific antibody is utilized again and is sprayed onto the T lines of the test strip so as to capture target thalli in a mixed sample, so that the thalli in the sample are detected rapidly and specifically; the detection assembly for detecting bacteria can be used for simultaneously detecting the food-borne pathogenic bacteria including the escherichia coli O157:H7 antibody, the listeria monocytogenes antibody and the salmonella typhimurium.
Owner:NANCHANG UNIV
Edwardsiella tarda recombinant subunit vaccine and application thereof
InactiveCN101991844AHigh protection rateAntibacterial agentsBacterial antigen ingredientsEscherichia coliEdwardsiella tarda
The invention relates to the field of molecular biology and immunology and particularly discloses an Edwardsiella tarda recombinant subunit vaccine as well as a preparation method and application thereof. The vaccine has a base sequence in a sequence table SEQ ID No.1. The preparation method of the Edwardsiella tarda recombinant subunit vaccine comprises the following steps of constructing a vaccine antigen expression plasmid pETXV21, converting the vaccine antigen expression plasmid pETXV21 into escherichia coli BL21 (DE3), recovering a recombinant protein after induced expression, mixing the recombinant protein with a strain B187, and dissolving the mixture in PBS (Phosphate Buffer Saline) to obtain a vaccine mixed liquor which has the function of immune protection to Edwardsiella tarda. The vaccine mixed liquor is intraperitoneally injected to fishes to achieve the purpose of immune protection.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
PAMP37-PR39 fused antibacterial peptide and preparation method thereof
InactiveCN104017085AImprove disease resistanceImprove securityAntibacterial agentsPeptide/protein ingredientsChemistryDisease
The invention relates to a PAMP37-PR39 fused antibacterial peptide and a preparation method thereof. Pig antibacterial peptides PAMP37 and PR39 are in series connected to form a fused antibacterial peptide; by utilizing preference of an escherichia coli codon, a DNA (deoxyribonucleic acid) sequence of the fused antibacterial peptide is designed, and is constructed into an expression vector, and then expressed and purified; an antibacterial activity result shows that the antibacterial activity of the obtained fused antibacterial peptide is greatly improved. The antibacterial peptide provided by the invention can improve disease resistance of pigs very well, can effectively substitute or reduce antibiotics for being used as a feed additive, so that animal product safety is improved, and therefore, the PAMP37-PR39 fused antibacterial peptide has an important practical application value.
Owner:科美博瑞科技(北京)有限公司
Mycoplasma hyopneumoniae multi-epitope mucosal vaccine
The invention relates to preparation and application of a mycoplasma hyopneumoniae multi-epitope mucosal vaccine. A mycoplasma hyopneumoniae membrane protein, an adhesive protein P97, a lipoprotein P65, a specific membrane protein P46, a B cell epitope, a Th epitope, a CTL epitope and a cholera toxin subunit B are taken as a vaccine frame structure, a pRSETA carrier is cloned in through flexible linker connection, then Escherichia coli is transformed, and fermentation, purification and preparation technologies are carried out, so that the mycoplasma hyopneumoniae multi-epitope mucosal vaccine with ideal immunogenicity is obtained. A self-made mucosal adjuvant is used in a preparation process, so that production and using processes of the vaccine are simpler and more convenient. Animal experiments show that the mycoplasma hyopneumoniae multi-epitope mucosal vaccine not only has good safety but also can stimulate effective mucosal immunity, humoral immunity and cellular immune reactions.
Owner:QINGDAO MINGQIN BIOLOGICAL TECH CO LTD
Flavonoid glycoside and isoquinoline alkaloid complex for inhibiting multi-drug-resistant staphylococcus aureus and preparation of carrier-free nano-drug of flavonoid glycoside and isoquinoline alkaloid complex
InactiveCN112010849AHigh antibacterial activitySensitiveAntibacterial agentsOrganic active ingredientsQuinolineFlavonoid glycosides
The invention provides a novel flavonoid glycoside and isoquinoline alkaloid complex structure, preparation, antibacterial application and three complex carrier-free nano-drugs with self-assembly performance. The structural general formula of the complex is shown in the specification, the complex has excellent selective antibacterial ability, and the activity of endophyte escherichia coli and probiotics bacillus subtilis and enterococcus faecium is not influenced while pathogenic bacteria staphylococcus aureus is killed; meanwhile, the compound has a good inhibition effect on multiple drug-resistant staphylococcus aureus, and is superior to norfloxacin, oxacillin, tetracycline and ciprofloxacin. The nano-drug formed by self-assembling the three complexes is uniform in form and good in dispersity and stability, gel or fiber is formed under the condition that auxiliary materials are not needed, and support is provided for development of a carrier-free pure drug delivery preparation.
Owner:BEIJING UNIV OF CHINESE MEDICINE
Biosynthesis method of alpha-lipoic acid, engineered strain and preparation method thereof
ActiveCN104450762AIncrease productionNo pollution in the processBacteriaMicroorganism based processesAlpha-Lipoic AcidToxic material
The invention discloses an alpha lipoic acid biosynthesis method, an engineered strain and a preparation method thereof. The biosynthesis method is as below: cloning an Escherichia coli lipD gene, an lplA gene, a metK gene and a lipA lipoic acid synthase gene; constructing a lipoic acid structural domain protein overexpression vector; cloning lplA into the vector highly expressing structural domain protein in series way structure to ensure full caprylyl; constructing a lipA expression vector or expression vector in series connection with metK; transforming the 4 vectors into appropriate Escherichia coli strains, regulating and comparing the medium composition and culture conditions, and promoting the conversion of caprylyl structural domain protein to lipoyl. The preparation method of alpha-lipoic acid provided by the invention employs combined low value chemical raw materials and pure biological production method, and has the advantages of little toxic substances in the production process, less pollution to the environment, high safety of the synthesized lipoic acid and high yield; and the alpha-lipoic acid has high activity.
Owner:GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
Process for producing D-serine
InactiveUS7186532B2Increase productionIncrease productivitySugar derivativesBacteriaEscherichia coliMicroorganism
The present invention relates to: a process for producing D-serine wherein a microbial cell which is modified to have a higher L-serine deaminase activity than Escherichia coli DH5α strain, a culture of said cell, or a processed product thereof is brought into contact with DL-serine in a DL-serine-containing medium to decompose L-serine, and the remaining D-serine is recovered from the medium; and a microorganism used for this production process. D-serine is a useful compound as a synthetic intermediate for useful medicaments such as D-cycloserine.
Owner:KYOWA HAKKO BIO CO LTD
Kit for identifying bacteria by use of molecular beacon-melting curve technology and application of kit
ActiveCN106834520AHigh homologyAvoid contamination riskMicrobiological testing/measurementAgainst vector-borne diseasesBacteroidesListeria monocytogenes
The invention discloses a molecular beacon and a kit for quickly identifying various clinically common bacteria and belongs to the technical field of microbiological detection. The sequences SEQ ID of the molecular beacon are as shown in v6p1, v6p2, v1p1 and v1r. Through comparison between various clinically common bacteria 16s ribosome RNA sequences in an NCBI gene sequence database and design of the molecular beacon at a tag sequence, clinically common 18 bacteria, including baumanii, A.hydrophila, burkholderia cepacia, citrobacter freundii, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus mirabilis, pseudomonas aeruginosa, staphylococcus epidermidis, staphylococcus aureus, salmonella, serratia marcescens and stenotrophomonas maltophilia, can be quickly identified. The invention further discloses the kit containing the molecular beacon and used for detecting various bacteria, and the kit can quickly and accurately identify various clinically common bacteria.
Owner:HANGZHOU DIAN BIOTECH CO LTD
Method for producing polysialic acid by using fermentation process, and extraction and refining method for polysialic acid
PendingCN111733092AReduce manufacturing costImprove fermentation yieldBacteriaMicroorganism based processesBiotechnologyEscherichia coli
The invention discloses a method for producing polysialic acid by using a fermentation process, and an extraction and refining method for polysialic acid. The method comprises the following steps: inoculating an escherichia coli strain SA-8 (CGMCC No. 5585) into a basic culture medium; transferring seeds into final fermentation; after entering a logarithmic phase, introducing a mixture of pure oxygen and air for ventilation; and after fermentation for 10 hours, gradually adjusting and lowering a pH value and a temperature, adjusting the pH value to 8.0 or above with Ca(OH)2 in the later periodof fermentation, stopping stirring and cooling within 18-19 hours, only continuously introducing common air for 1.8-2.2 hours, and finally conducting fermenting to obtain the polysialic acid which can be purified with anion exchange resin. The method is simple in process, saved in cost and reduced in energy consumption; the obtained polysialic acid is high in purity, does not contain endotoxin, can meet the production requirement that the polysialic acid is further hydrolyzed into monomer sialic acid (N-acetylneuraminic acid), and is applicable as a raw material for producing food, cosmeticsand medicines.
Owner:HIERAND BIOTECH CO LTD
Emodin modified polyester filament yarn and preparation method thereof
InactiveCN111235681AEnhance natural antibacterial effectLong antibacterial timeMonocomponent polyesters artificial filamentArtifical filament manufacturePolymer scienceSpinning
The invention provides a preparation method of emodin modified polyester filament yarn. The polyester filament yarn contains 0.1-5% of emodin. The preparation method of the polyester filament yarn comprises the steps of preparation of a functional modifier containing emodin, preparation of an emodin composite modifier, mixing and high-speed spinning. According to the emodin modified polyester filament yarn prepared by adopting the method, the adhesion of bacteria can be reduced, and the bacteriostasis rate on staphylococcus aureus, escherichia coli, candida albicans and other bacteria which are easily infected by a human body reaches 95.0-98.5%; the breaking strength of the polyester filament yarn is 6.0-6.2 cN / dtex, the elongation at break is 38.5-40.0%, after the prepared polyester filament yarn fabric is washed for 50 times, the bacteriostasis rate is still 95% or above, the tensile strength retention rate is 98% or above, and the fiber is quite excellent in washing resistance.
Owner:BESTEE MATERIAL (TSINGTAO) CO LTD
Alginate lyase and gene and application thereof
InactiveCN109777817ANothing producedMild reaction conditionsBacteriaMicroorganism based processesEscherichia coliBiotechnology
The invention discloses an alginate lyase and a gene and application thereof. The nucleic acid sequence of the alginate lyase gene is shown as SEQ ID NO:1; the amino acid sequence of the alginate lyase Alg3 encoded according to a nucleotide sequence is shown as SEQ ID N0:2. The invention also discloses a technical method for transforming a gene engineering strain of the alginate lyase gene into Escherichia coli by means of genetic engineering. The alginate lyase is prepared by connecting the alginate lyase gene with a vector, constructing tecombinant expression plasmid and transforming the Escherichia coli. The alginate lyase can specifically degrade sodium alginate to produce alginate oligosaccharide and can be used together with cellulase for hydrolyzing kelp to produce a water-soluble oligosaccharide product. Therefore, the alginate lyase can be used for preparation of alginate oligosaccharide; moreover, reaction conditions are mild, consumption of energy is low, no pollutants are produced, and the alginate lyase has an important industrial application value.
Owner:青岛海大生物集团股份有限公司
Recombinant escherichia coli for producing succinic acid and application thereof
The invention relates to the field of producing succinate by E. coli fermentation. Specifically, the invention provides an engineered recombinant E. coli for producing succinate, wherein said E. coli contains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinant E. coli for producing succinate, and a method of using the engineered recombinant E. coli for producing succinate.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Medical PP inorganic antibacterial composite and preparation method thereof
The invention discloses a medical PP inorganic antibacterial composite and a preparation method thereof. The medical PP inorganic antibacterial composite is prepared by use of the steps of weighing PP, divinyl benzene, a nano-zirconium phosphate silver-loaded antibacterial agent, an AC foaming agent, calcium stearate, PE, an antioxidant, paraffin, dicumyl peroxide, titanium dioxide, a surface treating agent, thiodipropionic acid dilauryl ester, azodicarbonamide, sodium benzoate and a titanate coupling agent in parts by weight, mixing all the components evenly and then extruding and pelletizing. The relative density of the obtained composite is 0.05-0.45 and the tensile strength of the composite is 2.5-4.5MPa; besides, the composite has the antibacterial rate of 99.5-99.9% to escherichia coli, 99.5-99.9% to staphylococcus aureus and 99-99.5% to pseudomonas aeruginosa, the bending strength of 11-15MPa, the degree of crosslinking of 45-55%, the impact strength of 3-5kJ / m<2>, and the bacteriostasis rate of 92-96% to salmonella typhimurium and Klebsiella pneumonia.
Owner:SUZHOU BEC BIOLOGICAL TECH
Upconversion-immunochromatography test strip used for detecting Escherichia coli O157:H7, and detection method thereof
InactiveCN107632156AReduce sensitivityWide detection rangeMaterial analysisEscherichia coliUpconversion luminescence
The invention provides an upconversion-immunochromatography test strip for detecting Escherichia coli O157:H7, and a detection method thereof. The test strip comprises a sample pad, a conjugate pad, an analysis membrane and an absorbent pad, the sample pad and the conjugate pad are glass cellulose membranes, the analysis membrane is a nitrocellulose membrane, the absorbent pad is a cellulose membrane, the analysis membrane is provided with a detection line and a quality control line, the sample pad, the conjugate pad and the absorbent pad are sequentially pasted on a PVC plate, the conjugate pad is immobilized with an upconversion luminescence nanoparticle-Escherichia coli O157:H7 monoclonal antibody IgG conjugate, the detection line on the analysis membrane is immobilized with an Escherichia coli O157:H7 antigen, and the quality control line is immobilized with MAbF1. The above upconversion nanomaterial-based immunofluorescence test strip has the advantages of low sensitivity, wide detection range, stable signal, no photobleaching, low background, sensitive signal, and accurate and reliable result.
Owner:HUNAN UNIV
Superbacteria antibiotic disinfectant, preparation method and purpose thereof
ActiveCN106234439APlay a continuous antibacterial and antibacterial effectImprove the bactericidal effectBiocideDead animal preservationBacteroidesMonilinia laxa
The invention provides a superbacteria antibiotic disinfectant, a preparation method and a purpose thereof. The antibiotic disinfectant comprises the following components by content: 1-100 g / L of a composite ion antibacterial material, 10-15 g / L of nano ZnO-modified dihydroxy diphenyl silane, 1-100 g / L of a film forming agent, 1-100 g / L of a thickening agent, 1-10 g / L of a plant extraction solvent, 1-10 g / L of a solubiliser, 1-10 g / L of a refrigerant, and the balance of water. The superbacteria antibiotic disinfectant can be used for killing methicillin-resistant staphylococcus aureus (super bacterium), escherichia coli, legionella bacillus, pyogenic coccus, hepatitis virus, candida albicans and staphylococcus aureus in various epidemic infectious diseases and bacterial spores, and is used for article surface and environment disinfection in high-speed rail, airplane, steamship, subway, hospital, school, kindergarten, large-scale stadium, large-scale entertainment facility and other public places as well as used for epidemic area disinfection.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI
Method for high-yield production of L-cysteine by metabolic engineering modification of corynebacterium glutamicum
The invention provides a method for high-yield production of L-cysteine by metabolic engineering modification of corynebacterium glutamicum. The method comprises the following steps: knocking out an L-cysteine desulfhydrase gene aecD while overexpressing a serine acetyltransferase gene cysE to obtain an L-cysteine accumulation strain; respectively overexpressing the serine acetyltransferase genescysE of escherichia coli and arabidopsis thaliana, and obtaining EcysE(M201R) most beneficial to L-cysteine synthesis after activity comparison; respectively overexpressing L-cysteine transporter proteins from escherichia coli and pantoea ananatis, and comparing the influences of different transporter proteins on the yield of L-cysteine to obtain a transporter protein Bcr most beneficial to L-cysteine secretion; promoting the synthesis of L-cysteine by improving the level of a precursor L-serine; and finally fermenting for 3-4 days by using 50-60 g / L glucose as a substrate, wherein the contentof L-cysteine reaches 800-1000 mg / L.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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