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Upconversion-immunochromatography test strip used for detecting Escherichia coli O157:H7, and detection method thereof

An immunochromatographic test paper, O157 technology, applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of not satisfying quantification and high sensitivity, and achieve the effects of high specificity, accurate and reliable results, and wide detection range.

Inactive Publication Date: 2018-01-26
HUNAN UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, the detection method of Escherichia coli O157:H7 is mostly limited to the colloidal gold test strip method, which cannot meet the needs of quantitative and high-sensitivity detection

Method used

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  • Upconversion-immunochromatography test strip used for detecting Escherichia coli O157:H7, and detection method thereof
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  • Upconversion-immunochromatography test strip used for detecting Escherichia coli O157:H7, and detection method thereof

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Embodiment 1

[0039] An upconversion-immunochromatography test strip for detecting Escherichia coli O157:H7, said test strip comprising a sample pad, a binding pad, an analysis membrane, a water-absorbing pad, and said sample pad and binding pad are glass cellulose membranes, The analysis membrane is a nitrocellulose membrane, the water-absorbing pad is a cellulose membrane, a detection line and a quality control line are provided on the analysis membrane, and the sample pad, binding pad, and water-absorbing pad are pasted on the PVC board in sequence;

[0040] The binding pad is immobilized with upconversion luminescent nanoparticles-Escherichia coli O157:H7 monoclonal antibody IgG conjugate;

[0041] Escherichia coli O157:H7 antigen is immobilized on the detection line on the analysis membrane, and MAbF1 is immobilized on the quality control line.

[0042] Further, the sample pad, the binding pad, the analysis membrane, and the water-absorbent pad are alternately laminated and pasted on t...

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Abstract

The invention provides an upconversion-immunochromatography test strip for detecting Escherichia coli O157:H7, and a detection method thereof. The test strip comprises a sample pad, a conjugate pad, an analysis membrane and an absorbent pad, the sample pad and the conjugate pad are glass cellulose membranes, the analysis membrane is a nitrocellulose membrane, the absorbent pad is a cellulose membrane, the analysis membrane is provided with a detection line and a quality control line, the sample pad, the conjugate pad and the absorbent pad are sequentially pasted on a PVC plate, the conjugate pad is immobilized with an upconversion luminescence nanoparticle-Escherichia coli O157:H7 monoclonal antibody IgG conjugate, the detection line on the analysis membrane is immobilized with an Escherichia coli O157:H7 antigen, and the quality control line is immobilized with MAbF1. The above upconversion nanomaterial-based immunofluorescence test strip has the advantages of low sensitivity, wide detection range, stable signal, no photobleaching, low background, sensitive signal, and accurate and reliable result.

Description

technical field [0001] The invention belongs to the technical field of pathogenic microorganism detection, and in particular relates to an upconversion-immunochromatography test strip and a detection method for detecting Escherichia coli O157:H7. Background technique [0002] Escherichia coli O157:H7 is an important zoonotic pathogenic microorganism. Humans infected with Escherichia coli O157:H7 may suffer from severe complications such as diarrhea, hemorrhagic colitis, and uremia, and severe cases may lead to death. O157:H7 has become a global public health problem because of its outbreak tendency, strong pathogenicity and lethality, and antibiotic treatment may aggravate the disease. [0003] ICA was used to detect human chorionic gonadotropin (HCG) in 1980, showing the advantages of good specificity and high sensitivity. Since then, researchers have continued to explore ICA technology, and now ICA can be widely used in the detection of biomolecules, infection factors and...

Claims

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Application Information

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IPC IPC(8): G01N33/569
Inventor 蒋健晖唐丽娟
Owner HUNAN UNIV
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