399results about How to "Improve fermentation yield" patented technology

L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

Production of fermentation products, such as ethanol and lactic acid in biofilm reactors by microorganisms immobilised on sterilised granular sludge.

The invention discloses a sinorhizobium meliloti strain capable of being applied to fermentation to generate vitamin B12, wherein the preservation number of the sinorhizobium meliloti strain is CGMCC No.9638. The invention also discloses a production and preparation method of vitamin 12 by the sinorhizobium meliloti strain in a fermentation manner. The method comprises a fermentation culture condition of the strain and an extraction method of the vitamin 12 in fermentation liquor; the method comprises the following steps: inoculating the strain on a solid culture medium into a triangular flask as a seed solution; inoculating the seed solution into a fermentation culture medium at 30 DEG C with 10% of inoculation amount; fermenting and cultivating at 200rpm for 200 hours, and then collecting the fermentation liquor; and adding a transforming agent to transform the fermentation liquor into cyano cobalt amine. The vitamin 12 is widely applied to the industries such as feeds, additives, medicines and cosmetics as a special factor in an adjusting organism growth process; and the requirement of the vitamin 12 rises year by year. The strain fermentation process is easy to control, so that the strain fermentation process is not affected by the factors such as seasons; the requirements on raw materials are simple; and fermentation production is developed into a main production mode.

The invention provides a method for fed-batch fermentation of L-lysine, which comprises the following steps of: introducing engineering bacteria for expressing pyridine nucleotide transhydrogenase variants into a first fermentation tank for culturing, and inoculating the obtained culture solution to a second fermentation tank for culturing; then, continuously feeding sugar in batches to the second fermentation tank; and then, continuously feeding sugar and nitrogen sources in batches to the second fermentation tank.

This invention relates to one method of improving fermentation efficiency of long chain diacid. In this method, normal alkane is the material, and animalcule fermentation method is adopted to produce correspondent diacid. Its major character is to add catastaltica, which includes halogeno-aliphatic acid, methyl fat acid and one or two kinds of its salt or ester, to reduce beta oxidation ability of bacterial strain. The concentration of the catastaltica is 0.01-5mmol / L. the added catastaltica can efficiently reduce beta oxidation ability of bacterial strain, lowers the alkane consumption, and improves greatly the fermentation efficiency, the acid-producing rapidity and is particularly suitable for long chain diacid.

An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

The invention belongs to the field of biological engineering and is aimed for solving the problem of mass scale plant diseases caused by fungus. The preparing process comprises subjecting a strong of Bacillus firmus (CGMCC No.0924) to second level fermentation culture under suitable condition, then carrying out filtering, salting out and column chromatography treatment.

The invention provides a fermentation method for preparing L-lysine products, which comprises the steps: an L-lysine culture solution is produced through three-level culture fermentation, and fine particles are coated by using fractional palm fat after spray drying. In addition, the invention also provides products prepared by the method, and the retention rate of L-lysine is larger than 15% within 24 hours.

The invention provides a method for production of ethanol by continuous fermentation of immobilized yeast cells, and the method comprises the following steps: (1) a fiber material is attached to a support frame, and is filled into a bioreactor; (2) yeast cells are immobilized in the fiber material in the bioreactor; (3) a fermentation medium is added into the bioreactor to begin ethanol fermentation; (4) when glucose in the fermentation medium is consumed, a fermentation liquid flows out of the bioreactor, the ethanol is collected, the fermentation medium is added, and then the step (4) is repeated. The method uses the fiber material for immobilization of the yeast cells for continuous fermentation to produce the ethanol, the fiber material as an immobilization material is stably in property, non-toxic, and good in adsorption and mass transfer effects, can be repeatedly used, is easy to achieve continuous production of the ethanol, and in a continuous fermentation process can greatly improve the ethanol yield.

The invention discloses a method for producing Nemadectin through fermenting. In the Nemadectin production process through fermenting, carbohydrates are intermittently or continuously added to a fermentation liquid to maintain the total sugar concentration of the fermentation liquid at 10-40g / L in order to carry out fermenting production when the total sugar concentration of the fermentation liquid is less than 30g / L. The method enables the Nemadectin fermentation unit to be improved by above 50%, simultaneously has the advantages of simple production operation, low equipment requirements and low cost, and is a technology suitable for the industrialized production.

The invention discloses a method for producing lipopeptide biosurfactant through fermentation. Bacillus subtilis is immobilized on a solid material and a dual-phase pH-controlled continuous batch fermentation technology is employed, so surfactant production efficiency is effectively improved.

The present invention describes an L-glutamic acid-producing bacterium which belongs to the genus Pantoea, Enterobacter, Klebsiella or Erwinia, wherein the bacterium has been modified by gene recombination to inactivate the rpoS gene. A method is also described for culturing the bacterium in a medium to cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

The invention provides a method for producing long-chain dicarboxylic acid by regulating and controlling the fermentation process through redox potential.The method comprises the steps of strain activating, seed culture and fermentation acid production.A fermentation system is a four-phase fermentation system formed by thalluses, sterile air, alkane and water, an ORP electrode is introduced into the fermentation system, and two-stage OPR control fermentation is achieved to produce the long-chain dicarboxylic acid by regulating the ventilatory capacity and stirring rotating speed in combination with an oxidizing agent and a reducing agent.According to the method, the ORP electrode is introduced in the process of producing the long-chain dicarboxylic acid through microbial fermentation, so that a two-stage ORP control strategy is achieved; the fermentation process of the long-chain dicarboxylic acid is regulated, and therefore the yield of the long-chain dicarboxylic acid and the stability of the fermentation system are improved; the fermentation yield is remarkably improved compared with a method that ORP is not controlled, even the fermentation yield is 4.6 times that of the method that the ORP is not controlled, and the production intensity can reach 1.78 g.h-1.L-1.

The invention discloses a solid ocean red yeast preparation which is mainly prepared from a composite protective agent and ocean red yeast bacterial sludge according to a mass ratio of (1.5-2.2):1, wherein the cell density of ocean red yeast in the solid ocean red yeast preparation is 200-400 billion / gram, the cell activity is 70-80%, and the protective agent comprises skim milk powder and cane sugar. The invention further discloses a preparation method of the solid ocean red yeast preparation, The preparation method comprises the following steps: firstly, performing high-density cultivation on the ocean red yeast in a cultivation mode of continuous liquid flow layer ventilation, and further drying by using a vacuum freezing drying method. According to the solid ocean red yeast preparation, the survival rate of the ocean red yeast can be increased to be 70-80%, the cell density in the preparation can be 200-400 billion / gram, and the preparation can be also used in aquatic product feeds as an additive as a product of the preparation is in a solid manner, long in expiration date and convenient to transport.

The invention discloses a method for increasing yield of erythromycin by virtue of a saccharopolyspora erythraea SACE_3986 gene. The method for increasing the yield of erythromycin by virtue of the saccharopolyspora erythraea SACE_3986 gene is that a saccharopolyspora erythraea erythromycin high yield engineering strain is obtained by virtue of an SACE_3986 gene with saccharopolyspora erythraea deleted in a genetic engineering way, the obtained bacterial strain can increase the yield of erythromycin when being used for fermentation, an erythromycin biosynthesis negative regulator SACE_3986 is screened under study, and an erythromycin high yield bacterial strain can be obtained by copying the SACE_3986 gene on a chromosome with saccharopolyspora erythraea deleted in the genetic engineering way, so that technical support is provided for increasing fermentation yield of erythromycin in industrial production.

The invention provides a multifunctional rice wind fermentation tank and a process method thereof. Semi-solid rice wine is prepared from sticky rice as a primary raw material. A cone-shaped stirrer and a round partition plate are arranged in the tank body, the round partition plate is arranged below the cone-shaped stirrer, spiral stirring blades are mounted on the cone-shaped surface of the cone-shaped stirrer, a connecting rod is mounted on the cone-shaped stirrer, penetrates through the top of the tank body and is driven by a motor, the round partition plate is composed of two semi-circularpartition plates which can turn around, and any one of the semi-circular partition plates can go up and down along with a tensile rod thereon to be horizontal or vertical to the other semi-circular partition plate. The multifunctional fermentation tank which integrates sticky rice soaking, steaming and cooling and separating steps before rice wine fermentation can finish all the work in th same container by a single person, so that the labor cost is lowered, the resources are saved, the work efficiency is improved, and the product quality is ensured. Multiple tanks are connected in series, sothat hot air and waste water are fully utilized, and the energy consumption cost is lowered.

The invention discloses a method for improving the fermentation yield of hyaluronic acid (HA), and aims to solve the problems that the yield of the HA is restricted under the influence of the physiological metabolic properties of streptococcus zooepidemicus serving as production strains, while the yield of the HA produced by a microbial fermentation method is required to be improved and the production cost is required to be reduced imperatively at present. The method comprises the following steps: slant culture, seed culture, fermentation culture, first-stage culture and second-stage culture. By utilizing the method, the fermentation yield of the HA can be further improved and the fermentation comprehensive cost is reduced in the mode of combining fed-batch culture and intermittent pH stress, so that the application of the HA in the field of medicaments, makeup and food healthcare is promoted. By utilizing the method, the conventional fermentation culture mode is subverted; the problem that the yield cannot be further improved in the method for producing the HA is effectively solved; the method has important significance to improve the fermentation yield of the HA, reduce the production cost of enterprises and promote the application of the HA.

The invention discloses aureobasidium pullulans dicarboxylic acid transport protein and a recombinant vector and application thereof. Amino acid sequence of the aureobasidium pullulans dicarboxylic acid transport protein is shown as SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9 or SEQ ID NO.10 while nucleotide sequence of the same is shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5. Overexpression of dicarboxylic acid transport protein in aureobasidium pullulans can increase yield of polymalic acid, and no free malic acid is detected in fermentation liquor, so that dicarboxylic acid transport protein gene is related to synthetic transport of the polymalic acid, and a foundation is laid for increasing yield of the polymalic acid.

The invention discloses a method for increasing the erythromycin yield by transforming the saccharopolyspora erythraea SACE_3980 gene. In saccharopolyspora erythraea, the gene SACE_3980 is subjected to transcription regulation by genetic engineering and through TetR-deficient family to obtain an engineering strain with high erythromycin yield; and by adopting the obtained strain for fermentation production of erythromycin, the yield can be remarkably increased, and a new technical support is provided for increasing the erythromycin yield in industrial production.

The invention discloses a method for sectional regulation and control for continuous strain culture. By adopting the method, nutrient substances are continuously replenished into a fermentation liquidto maintain strain metabolism, and at the same time, the fermentation liquid is continuously led out and is fed into a serial fermentation tank for further fermentation; to increase the strain metabolism velocity, parameters such as dissolved oxygen, pH values and replenished material constitution are respectively regulated and controlled at different stages. By adopting the method, the times ofstrain culture and equipment cleaning are reduced, the production process can be simplified, and the fermentation cost can be reduced. In addition, due to sectional regulation and control on the fermentation process, the yield of rhamnolipid can be increased, the fermentation production efficiency can be improved, the product batch stability can be improved, and the method is easily applied to industrial production.

The invention screens out lactobacillus parabrevis HX12-19 for high-yield gamma-aminobutyric acid. The lactobacillus parabrevis HX12-19 is preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCCM2017307. The lactobacillus parabrevis HX12-19 is obtained through isolating and screening out foodstuff materials, has high safety and can be used for fields such as food; traditional fermentation and bioconversion subdivision control are adopted, bio-enzyme conversion is fully utilized to obtain a product gamma-aminobutyric acid, the production efficiency is greatly improved, the cost is reduced, the fermentation yield can be up to 235g / L, so that the gamma-aminobutyric acid has productive competitiveness; a crystalline method is adopted to purify products, the purity can be up to 98%, and the lactobacillus parabrevis HX12-19 is suitable for industrial production.

The invention discloses a method of increasing the yield of spinosad by improving the fermentation condition of saccharopolyspora spinosa based on metabonomics. The method comprises the following steps: (1) determining intracellular metabolite of the saccharopolyspora spinosa; (2) analyzing the capability of the saccharopolyspora spinosa of producing the spinosad; (3) carrying out PLS analysis; and (4) carrying out process analysis. According to the method disclosed by the invention, the metabolite change law in the process of producing the spinosad by virtue of fermentation of the saccharopolyspora spinosa is revealed by utilizing the metabonomics means and combining multivariate statistics, and a metabolic change mechanism related to production of the spinosad is further explained; by analyzing the spinosad production capability and the metabolic level of the saccharopolyspora spinosa in different growth environments, the metabolin related to the production of the spinosad is found, thus providing a direction for optimizing the culture process of the saccharopolyspora spinosa and increasing the fermentation yield of the spinosad. The invention also provides new thinking and method for study in other culture technological processes of producing macrolides microorganisms.

The invention discloses a lucid ganoderma-probiotic composite fermentation product and a preparation method and application thereof. The lucid ganoderma-probiotic composite fermentation product comprises a lucid ganoderma fermentation supernatant, a probiotic fermentation liquid and functional nutritional ingredients. The preparation method comprises the following steps: fermenting lucidum ganoderma mycelia, carrying out centrifuging, taking the supernatant, and mixing the supernatant with the functional nutritional ingredients; then adding the probiotic fermentation liquid into a mixture obtained in the previous step, and carrying out fermentation; and finally, subjecting a fermentation product to inactivation or centrifugation to obtain the composite fermentation product. The composite fermentation product contains rich nutritional ingredients such as polysaccharides, polypeptides and organic acids and is easily and fully absorbed by the skin, and the composite fermentation product contains ingredients such as microelements which can promote repair and recovery of injured skin, so the composite fermentation product has a good skin care effect. The composite fermentation product is beneficial for skin health and is not polluted by enzymes, chemicals and the like; the whole fermentation system is mild in conditions; and the preparation method is simple to operate and controllable in cost, and is beneficial for large-scale production.

The invention discloses a method for improving the Pristinamycin (also named Peristamycin)output, in which the Pristinamycin is the fermentate of Streptomyces pristinaespiralis. By directly adding the macroreticular resinous adsorbent into fermentation liquor, the in-situ linkage of microorganism fermentation and product segregation can be carried out, which alleviates the Pristinamycin relaxation for production bacterium at a large degree and simultaneously avoids the probability that the Pristinamycin can be easily degraded in the later fermentation. By selecting macroreticular resinous adsorbent species, adding quantity, adding time and adding mode, the fermentation level of the said Pristinamycin can be increased to 1.0-2.5g / L, which can effectively improve the Pristinamycin output, accordingly it can reduce the Pristinamycin cost of manufacture.

The invention relates to a Welan gum producing marine strain, which belongs to Sphingomonas sp.WG, and has a preservation number of CCTCC No: M2013161. According to the present invention, the strain can be cultured in a liquid culture medium so as to obtain the Welan gum, and the production efficiency is high; the requirements of the strain of the present invention on the carbon source and the nitrogen source are low; the fermentation process comprises: (1) seed liquid culture and (2) fermentation production of Welan gum; the extraction and purification steps comprise: (1) fermentation broth pretreatment, (2) crude product precipitation, (3) impurity removing, and (4) drying so as to obtain the Welan gum product; with application of the marine strain to produce the Welan gum in the fermentation manner, the yield can be up to 33 g / L, and the extraction and purification yield can be up to 95%; and with the strain and the method of the present invention, the low-cost, high-efficiency and high-quality Welan gum production can be achieved.