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High yield microbe of 1,3-propylene glycol

A technology for microorganisms and microbial strains, applied in the direction of microorganisms, microorganism-based methods, enzymology/microbiology devices, etc., which can solve the problems of insufficiency and low performance

Active Publication Date: 2014-04-23
GUANGDONG TSINGDA SMART BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, wild Klebsiella pneumoniae (Klebsiella pneumoniae) ferments glycerol to produce PDO with low performance, which cannot meet the needs of modern industrial production

Method used

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  • High yield microbe of 1,3-propylene glycol
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  • High yield microbe of 1,3-propylene glycol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: Isolation and screening of Klebsiella pneumoniae

[0058] 1. Materials: 30 samples of soil and riverside sludge were randomly collected in Beijing.

[0059] 2. Medium:

[0060] (1) Enrichment medium (g L -1 ):

[0061] Peptone 5.0, beef extract 2.0, yeast powder 3.0, NaCl3.0, K 2 HPO 4 2.0, glycerin 60.0, calcium lactate 15.0, 0.4% bromocresol purple 2mL, pH7.0;

[0062] (2) Separation medium (g L -1 ):

[0063] Peptone 5.0, Beef Extract 2.0, Yeast Powder 3.0, K 2 HPO 4 2.0, glycerin 60.0, calcium lactate 15.0, 1% bromocresol purple 2mL, agar powder 18, pH7.5;

[0064] (3) Seed medium and fermentation medium: See Table 1 for configuration methods;

[0065] (4) Incline medium (g L -1 ): yeast extract powder 5, peptone 10, NaCl5, agar 15, pH7.0.

[0066] 3. Separation method

[0067] Put 1 g of soil sample into a 250 mL Erlenmeyer flask filled with 50 mL of enriched medium, and seal the bottle with six layers of gauze. 37 degrees Celsius, 150rp...

Embodiment 2

[0074] Embodiment 2: transmission electron microscope experiment

[0075] The Klebsiella pneumoniae ACR30 isolated and screened in Example 1 was inoculated into LB solid medium, cultured at 37 degrees Celsius for 24 hours, and then the cultured colonies were placed under a transmission electron microscope (Hitachi, Japan, H-7650B) down for observation. Among them, the parameters of the transmission electron microscope are as follows: resolution: 0.2nm (lattice image), accelerating voltage: 40kV-120kV, continuous magnification mode: x200-x600,000, low-magnification mode: x50-x1,000. see results figure 1 .

[0076] figure 1 A transmission electron micrograph of Klebsiella pneumoniae of the present invention is shown. Depend on figure 1 It can be seen that the Klebsiella pneumoniae capsule of the present invention is thinner and has more translucent colonies, and further, it proves that the strain ACR30 of the present invention has good performance and high production intens...

Embodiment 3

[0077] Embodiment 3: fermented liquid viscosity experiment

[0078] According to the following steps, Klebsiella pneumoniae ACR30 of the present invention obtained in embodiment 1 and the fermentation broth viscosity of control bacteria AC521 are compared:

[0079] Klebsiella pneumoniae ACR30 obtained in Example 1 and control bacteria AC521 cultures are configured to OD with deionized water 600nm 10 sample solution. Using an Ubbelohde viscometer with an inner diameter of 1.2 mm, the viscosity and filtration speed of the two groups of sample liquids were measured in a water bath at a water temperature of 30 degrees Celsius.

[0080] The experimental results are shown in Table 3 below.

[0081] Viscosity and filtration speed of bacterial classification ACR30 of the present invention and contrast bacterium AC521 of table 3

[0082]

[0083] As can be seen from the results in Table 3, the viscosity of the bacterial strain ACR30 of the present invention is significan...

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Abstract

The invention discloses a high yield microbe of 1,3-propylene glycol, and concretely discloses a separated microbe, a method and a system for producing PDO, wherein the separated microbe is Klebsiella pneumonia, and is preserved at the common microorganisms center of the CCCCM on 11th, July, 2013, a preservation number is CGMCC NO.M 7824, and the preservation name is ACR30. The Klebsiella pneumonia has thin capsule, and the PDO produced by using glycerin fermentation has the advantages of good performance and high production strength.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a high-yielding 1,3-propanediol microorganism, and more specifically, the invention relates to an isolated microorganism, a method and a system for producing PDO. Background technique [0002] 1,3-Propanediol (PDO), as a typical diol compound, is widely used in lubricants, dyes, inks, antifreeze, solvents and pharmaceutical industries, and can also be used to synthesize polymers such as polyurethane. However, due to production cost issues, its use scale is still small. [0003] PDO production methods can be divided into chemical synthesis and microbial fermentation. The chemical method route is that chemical raw materials undergo a series of chemical catalytic reactions to finally synthesize PDO; the biological method refers to the synthesis of PDO by microorganisms using renewable raw materials glycerol or glucose. Compared with the chemical process route, the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/18C12M1/00C12M1/34C12R1/22
Inventor 刘德华欧先金孙燕
Owner GUANGDONG TSINGDA SMART BIOTECH CO LTD
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