The invention belongs to the technical field of
glycated protein detection, and particularly relates to a method for detecting glycated
serum albumin by using a
boric acid affinity principle. The method is characterized by comprising the following steps: taking a certain volume of
bromcresol purple solution in a
cuvette; adding a serum sample to be detected into the
bromcresol purple solution; obtaining the
total protein concentration CT by measuring the
absorbance of 585 nm; adding a
phenylboronic acid adsorbent into the
cuvette to react with the BCP-GA; waiting until the adsorbent is completely deposited at the bottom of the
cuvette; and measuring the
absorbance of 585 nm to obtain the concentration Cng of the non-glycated
serum albumin, and calculating the proportion of the glycated
serum albumin through a formula (CT-Cng) / CT * 100%. According to the method, the
bromcresol purple is used for testing the total
albumin content under the alkaline condition, the
absorbance is reduced after the
albumin and the bromcresol purple are combined, the
albumin content and the absorbance decline value are in a
linear relation, all tests are completed through the absorbance
spectrophotometry, reagents are stable, detection is rapid, simple and convenient, and the method is completely compatible with general equipment in hospitals.