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Preparing method for cow mammitis streptococcus culture fluid and its use

A cow mastitis and preparation method technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems that cannot truly reflect the sensitivity of antibiotics, the price is very expensive to accept, etc., to achieve cheap equipment, expansion fast speed effect

Inactive Publication Date: 2008-01-09
南京市天邦生物科技有限公司
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Problems solved by technology

These methods not only need at least 3-5 days to get the results, but also have the necessary equipment, reagents and technical conditions for bacteriological identification, and the results of drug susceptibility tests with single colonies sometimes cannot truly reflect the sensitivity of the bacterial "population" to antibiotics sex
As for the automated drug sensitivity instrument and Etest method, it is difficult to be accepted by domestic veterinary clinics and dairy farms due to their high price

Method used

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  • Preparing method for cow mammitis streptococcus culture fluid and its use
  • Preparing method for cow mammitis streptococcus culture fluid and its use

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Embodiment Construction

[0017] 1. Preparation of EN culture medium:

[0018] Use a balance to weigh 10g of peptone, 5g of yeast powder, 10g of sodium chloride, 5g of glucose, and 0.5g of sodium azide, add them into 1L of water, stir to dissolve each component in water, and then adjust the pH value to 7.2 with sodium hydroxide solution. Then add 1.3ml of filter-sterilized 0.1% crystal violet aqueous solution and 1.5ml of 1% bromocresol violet aqueous solution, and sterilize at 121°C for 15 minutes to prepare liquid EN culture medium, which is stored at 4°C for later use.

[0019] Add 15 grams of agar powder to the above-mentioned per liter of liquid EN culture solution to form an EN agar plate. Store at 4°C for later use.

[0020] 2. Rapid identification of streptococci:

[0021] According to the clinical examination or the results of the California Mastitis Test (CMT), it is preliminarily judged that the cows have clinical or latent mastitis. The first three handfuls of milk are squeezed out by con...

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Abstract

The preparation process of cow mammitis streptococcus culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, yeast powder 5 g, sodium chloride 10 g, glucose 5 g and sodium azide 0.5 g in water of 1 L; regulating pH to 7.2 with sodium hydroxide; adding bacteria-free 0.1% concentration crystal violet water solution in 1.3ml and 1% concentration bromocreasol purple water solution in 1.5ml; sterilizing at 121deg.c and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis streptococcus and antibiotic sensitivity test.

Description

technical field [0001] The invention belongs to the field of diagnosis and prevention of animal epidemics. technical background [0002] Dairy cow mastitis has a high incidence and great harm, and is one of the most important diseases affecting the development of the dairy industry in the world. According to the International Milk Federation, about 50% of cows suffer from mastitis, of which about 2% suffer from clinical mastitis and 48% suffer from recessive mastitis. The incidence rate of clinical mastitis in my country is 33.41% (9.7%-55.6%), and the detection rate of recessive mastitis is 50-80%. Clinical mastitis has obvious clinical symptoms such as decreased milk production, breast swelling, and milk deterioration, while subclinical mastitis has no visible changes in the appearance of breasts and milk, but often causes the quality of raw milk to decline, threatening people's health. healthy. It is estimated that the annual economic loss caused by mastitis in my coun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
Inventor 孙怀昌仇华磊钱科张鑫宇
Owner 南京市天邦生物科技有限公司
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