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Bis(4-pyridine)cresol purple sultones as well as preparation method and application method thereof

A technology of violet sultone and violet sulfonic acid, which is applied in the field of albumin detection, can solve the problems of high detection value of albumin, increased light absorption value, inconvenient use, etc., and achieves simple operation, accurate detection results, and increased detection sensitivity. Effect

Inactive Publication Date: 2018-11-02
SHANDONG ZHUSHI PHARMA GRP CO LTD
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Problems solved by technology

However, this has caused the increase in the absorbance value in the detection of clinical serum, and the detected value of albumin is relatively higher than the true value, which has brought inconvenience to people's use.

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  • Bis(4-pyridine)cresol purple sultones as well as preparation method and application method thereof

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Embodiment Construction

[0021] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0022] A kind of two (4-pyridine) cresol purple sultone, its structural formula is

[0023] The preparation method of described two (4-pyridine) cresol purple sultone, concrete steps are as follows:

[0024] Step 1, add 1080mg compound 1-bromocresol purple (1eq, 2mmol), 1.542g potassium phosphate (3.6eq, 7.2mmol), 327mg tetrakis (triphenylphosphine) palladium (0.14eq, 0.28 mmol) and 1.07mg 4-pyridine boronic acid alcohol ester (2.6eq, 5.2mmol), then add 20ml N,N-dimethylformamide and 4ml water, heat to 100 degrees Celsius under the protection of argon, and carry out TLC to monitor the reaction process And the volume ratio of petroleum ether and ethyl acetate in the developer is 5:1, and the reaction is stopped after 6 hours to obtain the first reaction solution;

[0025] Step 2: The first reaction solution was concentrated and evaporated to dry...

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Abstract

The invention discloses bis(4-pyridine)cresol purple sultones as well as a preparation method and an application method thereof. The structural formula is shown in the description. The structure of the compound is to replace the bromine with 4-pyridine group on the basis of a bromcresol purple nuclear parent, so that a detection function of bromcresol purple sultones can be kept. The 4-pyridine group is used for substituting the bromine atoms to design and synthesize bis(4-pyridine)cresol purple sultones, so that bis(4-pyridine)cresol purple sultones has the advantage in detecting the contentof albumin in serum as follows: firstly, the poor water solubility of the bromcresol purple compound is changed, the water solubility of the compound is improved, and the detection sensitivity is improved; and secondly, bis(4-pyridine)cresol purple sultones contains no bromine atoms, so that the non-specific hydrophobic combination between the bromine atoms and various proteins can be reduced, andbis(4-pyridine) cresol purple sultones has better specificity for combining the albumin than that of the dye bromcresol purple compound, and can be used for detecting the content of the albumin.

Description

technical field [0001] The invention relates to the field of albumin detection, in particular to bis(4-pyridine)cresol violin sultone. Background technique [0002] The bromocresol purple method (BCP method) is a method for determining the content of albumin, which was mainly established in the 1970s. The principle of the experiment is that in the presence of Brij-35 in the buffer solution of pH 5.2, bromocresol purple (BCP) can combine with albumin to form a green complex, and the absorbance at a wavelength of 603nm is proportional to the concentration of albumin. Proportional, compared with the same treated albumin standard, the albumin content can be obtained. [0003] The dye method is used to determine the content of albumin in serum, which is easy to operate and is currently used by most clinical laboratories. However, there are many kinds of proteins in human serum, and the composition is extremely complex. Bromocresol green and bromocresol violet, two similar dyes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D411/14G01N21/33
CPCC07D411/14G01N21/33
Inventor 朱坤福祝蕾薛飞
Owner SHANDONG ZHUSHI PHARMA GRP CO LTD
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