69 results about "Bromcresol Purple" patented technology

The invention discloses a kit test method of beta-lactam antibiotics residue in milk, comprising the following steps: performing once activation to bacillus stearothermophilus, then culturing the activated bacillus stearothermophilus in a production culture medium at 50-65 DEG C for 24-36h while stirring in a rotation speed of 100-150rpm, centrifugalizing to collect bacteria mire, adding agarose bromcresol purple solution, mixing evenly to ensure the number of spore in each kit reach 1.00*10<7>-2.50*10<8>cfu, subpackaging the spore in porous reaction vessels at 60-70 DEG C, covering aluminum-plastic film, placing upside down, sealing, placing the vessels in aluminum-plastic packages and storing in dark place while keeping from wet. The kit has the advantage of easy operation, high sensibility, good repeatability, low cost and high throughput, can be stored at 4-8 DEG C for 12-18 months and can be used to test beta-lactam antibiotics residue in milk.

The invention relates to a rice metabolism detecting reagent and method, concretely used in the condition of detecting rice metabolism. It mainly takes and dissolves nitrazine yellow/chlorophenol red, p-nitrophenol, bromocresol green/bromothymol blue/resorcinol blue, bromocresol purple, and hematoxylin/cresol red/bromophenol red/methyl red into alcohol, and mixing them uniformly to obtain finished product of raw reagent liquid. As detecting, taking a raw reagent liquid and adding distilled water to dilute the raw reagent liquid so as to form a liquid reagent, adding rice to the liquid reagent to react, observing the colors of rice and solution, judging the metalolism degree of the rice detected, the metabolism situation and color of the rice corresponds to green and passes through yellow and salmon pink into red region, where the green expresses new rice, yellow expresses old rice and salmon pink expresses deeply aged rice. The reagent of the invention is lower-cost and has simple manufacturing process; the operation is quick, accurate and convenient; the detecting method is simple, able to be used in both qualitative detection and quantitative detection.

The invention discloses an amniotic fluid detection indicator composition, preparation thereof and a corresponding amniotic fluid detection pad. The amniotic fluid detection indicator composition is used by taking one or two of water, ethyl alcohol, acetone and N,N-dimethylformamide as a solvent or solvents, taking one or more than one of pulullan, sodium alginate, ethyl cellulose and sodium carboxymethylcellulose as a film-forming agent or film-forming agents, taking dioctyl phthalate as a plasticizer, taking ethylene glycol, glycerol and glycol ethylene ether as wetting agents, taking bromothymol blue sodium salt, bromoxylenol blue, sodium alizarine sulfonate and bromcresol purple as indicators, preparing an amniotic fluid detection indicator at the normal temperature, then coating a medical sanitary pad with the amniotic fluid detection indicator, and drying the medical sanitary pad to prepare an amniotic fluid detection pad. The amniotic fluid detection indicator composition is convenient to use, not easy to decolor and more easily acceptable to patients, and is capable of distinguishing amniotic fluid and urine; the whole process is simple to operate; the large-scale production is easy to implement.

The invention discloses a quick detection method for antibiotic residue in raw milk and belongs to the technical field of biology. According to the quick detection method disclosed by the invention, frozen, dried and stored geobacillus stearothermophilus is added into nutrient gravy agar culture medium to be activated and cultured; the activated geobacillus stearothermophilus is inoculated into phosphate buffer solution to obtain suspension liquid; the geobacillus stearothermophilus suspension liquid is added into bromcresol purple peptone culture medium to obtain test culture medium; the test culture medium is evenly mixed with raw milk to be detected according to volume ratio of (1:2) to (1:3) to be cultured in water bath in 75 DEG C to 80 DEG C for 2.0h to 2.2h, and color change of culture medium is observed. According to the quick detection method disclosed by the invention, system color change reaction time is greatly shortened by adjusting water bath culture temperature to 75 DEG C and coordinating corresponding reasonable volume ratio of the test culture medium to the raw milk, the time is shortened by about 20%, and detecting efficiency is further improved.

The invention discloses a rapid diagnosis reagent for mastitis of cattle, sheep and pig, and the components and the weight per cent is: Sodium dodecyl benzene sulfonate 2.5%-thirty%, sodium hydroxide 1%-15.5%, alternate sodium nitride 0.05%-0.5%, bromocresol purple 1%-10%, and water 45%-95%. The invention also discloses the preparation method for the reagent that is making up water solution with sodium hydroxide, alternate sodium nitride and adding sodium dodecylsulfate, bromocresol purple according to the ratio, whisking and mixing to make it fully dissolving. The invention has fast speed, low cost, high accuracy, repetitiveness and good stability. It is easy to operate, and has broad application range.

The invention discloses an electrochemical sensor for simultaneous detection of DNA bases, relates to the electrochemical detection of DNA and solves the problems that an existing electrochemical sensor is difficult to realize simultaneous sensitivity analysis and stability detection of four DNA bases, the electrochemical sensor is easily polluted by a sample, complex and expensive, and complicated to operate in an existing method, a potential window is narrow, the response current to a pyrimidine base is low and the stability is poor. The bases are detected simultaneously through the following steps by the electrochemical sensor: I, preparing an oxidized graphene and bromcresol purple solution; II, preparing a graphene-modified electrode; III, preparing a bromcresol purple/graphene composite modified electrode; IV, detecting an electrochemical signal of a four-base mixed solution by using the composite modified electrode, and analyzing and determining a peak position where the electrochemical signal is located; V, carrying out electrochemical detection on the base mixed solution to obtain a differential pulse voltammetry diagram with the change of concentration, and establishing an electrochemical signal change law diagram of the four DNA bases. The invention is applied to the field of electrochemical sensors.

The invention belongs to the technical field of agricultural analysis, specifically relates to a special ammonia indicator for analyzing ammonia content by flow injection instruments. An import flow injection instrument is used for analyzing the ammonia contents of samples such as animal and plant life, food, water and soil, which has been normally-used instrument device and technology in domestic universities and colleges as well as scientific research, animal and plant life and food detection and check units. However, the most important reagent that is ammonia indicator for analyzing ammonia content was monopolized by foreign companies all the time, and the cost is expensive. The invention provides the proper mass ratio of prepared special ammonia indicators of flow injection instruments that are cresol red, bromcresol purple and bromothymol blue. The main technical targets of the special ammonia indicator in the invention all meet analysis requirements, and the cost of the reagent is 3.3% of that of contrast reagent.

The invention relates to a long-chain alkyltrimethyl ammonium sensitizing mixed reagent paper for measuring ammonia in air and preparation method thereof, belongs to the field of rapid environmental detection and industrial hygiene. The test paper is loaded with bromocresol purple sodium salt, bromothymol blue sodium salt, and surfactant long-chain alkyl trimethyl ammonium on the base paper according to the mass ratio, bromocresol purple sodium salt: bromo thymol blue sodium salt: surfactant long-chain alkyl trimethyl ammonium = (4~8): (6~10): (0.02~0.2). The preparation method comprises the following steps of: preparing the soaking solution according to the load reagent; soaking the base paper; drying and encapsulating. The test paper is made to change from the yellow to blue-violet basedon the reaction between ammonia gas in combination with bromocresol purple sodium salt and bromothymol blue sodium salt. The color change is obvious, the color of the test paper reaction is clear, itis easy to observe and measure, and the precision is high. The long-chain alkyltrimethyl ammonium makes the bromocresol purple sodium salt and the bromothymol blue sodium salt disperse better, and makes its uniformity on the filter paper better, thus the length of the test paper discoloration band is increased, and the sensitivity is increased.

The invention belongs to the field of microorganisms and in particular relates to a staphylococcus aureus chromogenic culture media. The culture media comprises the following components: a trisaccharide iron (TSI) agar culture media, 12 g/L casein peptone, 6 g/L mannitol, 2.0 g/L dipotassium hydrogen phosphate, 0.5 g/L potassium dihydrogen phosphate, 8 g/L glycine, 12 g/L sodium pyruvate and a 0.16 g/L color developing agent, wherein the pH value is 7.2-7.4. According to the staphylococcus aureus chromogenic culture media, 1 L of the TSI agar culture media contains 15.0 g of tryptone, 10.0 g of soybean peptone, 5.0 g of yeast powder, 4.0 g of sodium chloride, 10.0 g of sucrose, 0.2 g of ferrous sulfate, 0.3 g of sodium thiosulfate, 15.0 g of agar, 0.04 g of bromcresol purple and the balance of distilled water. The staphylococcus aureus chromogenic culture media is simple in component, convenient to prepare, low in cost and favorable for popularization and application; staphylococcus aurei are high in growth speed, low in aberration rate and convenient to observe and analyze.

The invention discloses a method for detecting mechanical injuries for fresh products, aiming at providing a method for detecting micro-mechanical injuries on an agricultural product surface. The method comprises the following steps of: adding gelatin to water so as to prepare gelatin coatings, and dropping bromcresol purple indicator or bromothymol blue indicator to the gelatin coatings; covering the gelatin coatings on the agricultural product surface to form a coating film for 24 hours, and then taking off the coating film; and using a microscope to observe color-changing condition of the coating film to find out the injuries where distinctive color change appears. The method, which uses transparent coatings containing acid-base indicator to perform film coating on leaf vegetables so as to detect the micro-injuries through post-film microscopic observation and photograph, belongs to pioneering researches, thus having important practical significance. By using the method for detecting the mechanical injuries for the fresh products, the degree of the fresh products and the approximate injury time can be fast detected, so that the products can be evaluated, and storage and sales of the fresh products are guided; meanwhile, the recovery of the fresh products can be guided, thereby reducing the mechanical injuries so as to reach the final purpose of reducing proportion of goods damaged.

The invention relates to stability augmentation test paper for determining ammonia in air by a drying method and preparation and detection methods thereof, and belongs to the fields of environmental monitoring technology and industrial hygiene. The stability augmentation test paper for determining ammonia in the air by the drying method is the test paper loaded with a bromocresol purple-bromothymol blue mixed indicator and a cationic surfactant on base paper, which has a mass ratio of bromocresol purple: bromothymol blue: cationic surfactant = (0.1-0.55): (0.025-0.2): (X-X'). An X-X' value varies depending on the surfactant. The preparation method comprises the following steps of: preparing a soaking liquid according to a reagent ratio; soaking the base paper; and drying and carrying out plastic packaging. The detection method comprises the following steps of: detecting the reaction of ammonia in the air and a mixed reagent of the test paper; turning from yellow to bluish violet; and obtaining the concentration of the ammonia in the air of a detection area according to the relationship between the length of a color changing belt of the test paper and the ammonia concentration. Thedetection method can be used to measure the ammonia concentration in the air of a workshop or a production site or the emergency monitoring of the ammonia leakage.

The invention discloses a pH value testing liquid with the testing range of 4.6-7.6 and a preparation method of the pH value testing liquid. The invention provides a testing liquid which is convenient in testing, rapid in developing, good in stability and hard to splash and leak, and a preparation method of the testing liquid. The testing liquid comprises the following components by weight percent: 50-60% of an organic solvent, 0.5-3% of a thickening agent, 0.5-1% of an antioxidant, 1-2% of ethoxychrysoidine, 2-3% of litmus, 0.5-1% of bromcresol purple, 0.5-1% of neutral red, 0.2-0.5% of methylene blue and the balance of distilled water. After the testing liquid is used, the gradient change of agent color can be displayed when the accuracy change of the pH value difference which can be observed visually is 0.2; the testing liquid is convenient in testing, rapid in developing, good in stability, hard to splash and leak and low in price, is suitable for the quick test in the pH value change range outside a laboratory, and can be used in the fields of food safety, medicine detection, environmental protection, water quality monitoring, industrial production, criminal assay and the like which need to rapidly and accurately testing the pH value of solution to be tested outside the laboratory.

The invention belongs to the microbial field, especially relates to a lactose bile salt fermentation culture medium, and is characterized in that the culture medium consists of an EC broth, 5.0 g/L of a bile salt from ox, 0.065 g/L of bromcresol purple, 1.0 g/L of ferric citrate, and 15.0 g/L of agar, wherein the pH value is 7.3-7.5; the EC broth contains 20.0 g of tryptone, 10.0 g of lactose, 5.0 g of sodium chloride, 4.0 g of dipotassium hydrogen phosphate, and the balance distilled water in each liter of the EC broth. The culture medium has the advantages of simple formula, convenient preparation operation, low cost, and favorable popularization and application; bacteria grow rapidly, and the mutation rate is low; and observation and analysis are convenient.

The invention belongs to the field of clinical medicine and environmental health monitoring and particularly relates to test paper for detecting carbon dioxide and a preparation method of the test paper. The test paper is prepared by using a following method comprising the steps: A, dissolving ethyecellulose in acetone and agitating; adding bromothymol blue, bromcresol purple, 2-ethyl hexyloxy propylamine, sodium sulfafe decahydrate, polyethylene glycol 600, distilled water, calcium carbonate and silicon dioxide; agitating and mixing uniformly so as to obtain a solution; putting the solution into a closed container and standing for a period of time at a certain temperature condition; B, namely preparing a sodium hydroxide solution and putting a piece of paper with the good water-absorbing quality into the solution to be immersed sufficiently; then drying the paper in a drying oven; C, adding a certain amount of the distilled water in the solution obtained in the step A until the solution becomes bluish violet; dripping the solution to the paper treated in the step B and putting the paper into the drying oven to the dried. The test paper is convenient to use, low in construction cost and sensitive in detection, and can be widely applied to the field of the clinical medicine and the environmental health monitoring.

The invention provides a rapid screening method for high-yield lipase bacteria, belonging to the field of biotechnology. The invention further provides a high throughput screening method by which a discoloration reaction is carried out on a bromcresol purple acid-base indicator by utilizing the pH change of a fatty acid and establishes bacterial strains for generating lipase in a 96-well plate. The method is characterized in that the operation is simple, fast, accurate, high-throughput, etc. The invention relates to a method for rapidly screening the lipase bacteria. The technological difficulties are that 1, the prior art needs a manual measurement method, and adopts a diameter ratio calculation to obtain target bacteria that we need, and 2, in the process of cultivating induced bacteria, observation needs to be conducted from time to time so as to avoid exceeding the time limit (in general, 6 days is needed). The solution is that 1, a 96-well plate screening method is adopted so long as a microplate reader is used for measurement at the maximum absorption wavelength position thereof, and the data are more accurate, and 2, when the high throughput screening method is adopted, only 35 hours are needed from the beginning to the end, the process is simple and convenient, and observing at all times is not needed.